We narrowed to 11,909 results for: pcDNA

1. pcDNA3.1+FLAG-KrasnatG12D

   Plasmid

   #206842

   Purpose

   To express mouse Kras encoded by native mouse codons and with a G12D mutation with an N-terminal FLAG tag.

   Depositor

   Christopher Counter

   Article

   Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755

   Insert

   Kras (Kras Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed Glycine 12 to Aspartic Acid

   Promoter

   Available since

   Oct. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCDNA4.TO-ORF52-2xCSTREP

   Plasmid

   #136212

   Purpose

   Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF52

   Depositor

   Britt Glaunsinger

   Article

   Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

   Insert

   ORF52
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-TORCAR-NLS

   Plasmid

   #64931

   Purpose

   Nuclear-targeted FRET biosensor for monitoring mTORC1 kinase activity.

   Depositor

   Jin Zhang

   Article

   Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

   Insert

   TORCAR-NLS (EIF4EBP1 Synthetic, Human)
   

   Use

   Tags

   Cerulean, Nuclear localization signal (NLS), and …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1 (-) hYAP 1-2alpha

   Plasmid

   #124143

   Purpose

   Protein expression for funtional studies of cell growth promotion

   Depositor

   Megan Finch-Edmondson, Marius Sudol

   Article

   Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   None (Wild Type)

   Promoter

   Available since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCDNA3.0_nucNanobody IRES EGFP

   Plasmid

   #136619

   Purpose

   Plasmid encoding nuclear localization of the Enhancer nanobody for GFP as a SNAP-Tag-fusion and EGFP

   Depositor

   Kai Johnsson

   Article

   Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

   Insert

   SNAP-Tag-EnhancerNb-NLS (IRES EGFP)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. 896 pcDNA3 T7 Akt1

   Plasmid

   #9003

   Depositor

   William Sellers

   Article

   Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

   Insert

   Akt1 (AKT1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3-CBX1mut-PA-mCherry

   Plasmid

   #138251

   Purpose

   Triple-mutant CBX1 chromodomain (residues 20-73) tagged with photoactivatable mCherry (PAmCherry). Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1.

   Depositor

   Jin Zhang

   Article

   Hard et al Sci Adv. 2018 Nov 7;4(11):eaau1447. doi:

   Insert

   CBX1mut-PAmCherry
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-pri-let-7a-1

   Plasmid

   #51377

   Depositor

   Narry Kim

   Article

   Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

   Insert

   pri-let-7a-1 (MIRLET7A1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA 3.3 EGFP

   Plasmid

   #115786

   Purpose

   Expresses EGFP in mammalian cells

   Depositor

   Harald Junge

   Article

   Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-ALK3 Q233D

    Plasmid

    #80874

    Purpose

    mammalian expression of ALK3 Q233D

    Depositor

    Aristidis Moustakas

    Article

    Raja et al Oncotarget. 2016 Jan 12;7(2):1120-43. do

    Insert

    ALK3 (BMPR1A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Q233D (constitutively active)

    Promoter

    CMV

    Available since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3-RIIα-Dronpa

    Plasmid

    #182331

    Purpose

    PKA RIIα subunit-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.

    Depositor

    Jin Zhang

    Article

    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

    Insert

    RIIalpha-Dronpa (PRKAR2A Human)
    

    Use

    Tags

    Full-length human PKA type 2alpha regulatory subu…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCDNA3 IRF9-STAT2C

    Plasmid

    #37544

    Depositor

    Curt Horvath

    Article

    Kraus et al J Biol Chem. 2003 Apr 11;278(15):13033-8

    Insert

    IRF-9 STAT2 C (IRF9, STAT2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1(-)-HA-SrtAstrep (pKS77)

    Plasmid

    #50030

    Purpose

    Expression of cytosolic S. pyogenes SrtA in mammalian cells

    Depositor

    Hidde Ploegh

    Article

    Strijbis et al Traffic. 2012 Jun;13(6):780-9. doi: 10.1

    Insert

    SrtA(strep)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    Dec. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-His-SUMO3

    Plasmid

    #159325

    Purpose

    Mammalian cell expression of mouse His-SUMO3

    Depositor

    Jianjun Zhao

    Insert

    His-SUMO3 (Sumo3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA-HIRA-polyA

    Plasmid

    #59781

    Purpose

    Express H3.3 chaperone HIRA in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    HIRA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA5/FRT/TO V5 HSPA14

    Plasmid

    #19515

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    heat shock 70kDa protein 14 (HSPA14 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1 Flag mHMGB2

    Plasmid

    #31610

    Depositor

    Yasuhiko Kawakami

    Article

    Taniguchi et al Proc Natl Acad Sci U S A. 2009 Sep 29. 1

    Insert

    HMGB2 (Hmgb2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA5FRT/TO-UBXD8-Strep-HA

    Plasmid

    #113477

    Purpose

    Expression of human UBXD8 with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    UBXD8 (FAF2 Human)
    

    Use

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HA-UBE2E1 C131A in pcDNA3.1

    Plasmid

    #118214

    Purpose

    Expresses HA-UBE2E1 C131A catalytic mutant in mammalian cells

    Depositor

    Cynthia Wolberger

    Article

    Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

    Insert

    UBE2E1 (UBE2E1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed cysteine 131 to alanine

    Promoter

    Available since

    Oct. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pY011 (pcDNA3.1-hLb2Cpf1)

    Plasmid

    #69983

    Purpose

    Expresses humanized Lb2Cpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hLb2Cpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCDNA3-hTLR3-VenusFlag

    Plasmid

    #197636

    Purpose

    Human CD283 protein expression in mammalian cells

    Depositor

    Haian Fu

    Insert

    TLR3 (TLR3 Human)
    

    Use

    Tags

    Venus-Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3-DAKAP-μDpTT

    Plasmid

    #182324

    Purpose

    Mitochondria-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

    Depositor

    Jin Zhang

    Article

    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

    Insert

    DAKAP-μDronpa-TagRFP-T
    

    Use

    Tags

    N-terminal 30 amino acids from DAKAP1

    Expression

    Mammalian

    Mutation

    Dronpa contains H193T mutation.

    Promoter

    CMV

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1-TIE-V5/HIS

    Plasmid

    #205612

    Purpose

    Expression of human TIE receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    TIE receptor tyrosine kinase (TIE1 Human)
    

    Use

    Tags

    V5-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA5/FRT/TO_Lifeact_HaloTag7

    Plasmid

    #175535

    Purpose

    Expression of HaloTag7 at the actin filaments of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Lifeact-HaloTag7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3puro-mGFP-TOP3B

    Plasmid

    #225470

    Purpose

    Express mEGFP-fusion protein of TOP3B

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    TOP3B (TOP3B Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3-delta133HtrA-EGFP

    Plasmid

    #14124

    Depositor

    L. Miguel Martins

    Article

    Martins et al J Biol Chem. 2002 Jan 4. 277(1):439-44.

    Insert

    HtrA2 (HTRA2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deletion of the first 133 amino-acids

    Promoter

    Available since

    March 7, 2007

    Availability

    Academic Institutions and Nonprofits only

27. pcDNA-FLAG-MYO1D

    Plasmid

    #125125

    Purpose

    Expresses human FLAG-MYO1D

    Depositor

    Bruce Beutler

    Article

    McAlpine et al Dis Model Mech. 2018 Sep 27;11(9). pii:

    Insert

    MYO1D (MYO1D Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3-myc-Ataxin3Q28

    Plasmid

    #22125

    Depositor

    Henry Paulson

    Insert

    Ataxin 3 (ATXN3 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

29. pcDNA3.1(+)-OTI-TCRB

    Plasmid

    #131036

    Purpose

    Expresses OT-I TCRB

    Depositor

    Jeff Hammerbacher

    Article

    Aksoy et al bioRxiv

    Insert

    TCRB (Tcrb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/T7

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA6 MCV sTco

    Plasmid

    #40201

    Depositor

    Patrick Moore

    Article

    Shuda et al J Clin Invest. 2011 Sep;121(9):3623-34.

    Insert

    MCPyV sT codon optimized
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3.1 3xF-NHP2

    Plasmid

    #126871

    Purpose

    Expresses 3xFlag NHP2

    Depositor

    Kathleen Collins

    Article

    Egan et al Mol Cell Biol. 2010 Jun;30(11):2775-86.

    Insert

    NHP2 (NHP2 Human)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3 Hexim2 (P#1897)

    Plasmid

    #14663

    Depositor

    Matija Peterlin

    Article

    Barboric et al EMBO J. 2005 Dec 21. 24(24):4291-303.

    Insert

    Hexim2 (HEXIM2 Human)
    

    Use

    Tags

    His and Xpress

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

33. pcDNA3.1-YFP-KRAS4B

    Plasmid

    #112718

    Purpose

    Expresses YFP-KRAS4B fusion protein used for FRET studies in eukaryotic cells.

    Depositor

    Kenneth Westover

    Article

    Ambrogio et al Cell. 2018 Feb 8;172(4):857-868.e15. doi

    Insert

    YFP-KRAS4B (KRAS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3.1-N-Myc_DW164AA USP7

    Plasmid

    #131252

    Purpose

    Mammalian expression of N-terminally Myc-tagged DW164AA USP7

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Contains a point mutation that converts USP7 aspa…

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA5-FRT-TO-FH-Nsp16

    Plasmid

    #157724

    Purpose

    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    FH-Nsp16 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Flag-His8

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. pcDNA3/Myc-DNMT3B3

    Plasmid

    #37546

    Depositor

    Arthur Riggs

    Article

    Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

    Insert

    DNMT3B3 (DNMT3B Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3-myc3-Tsc1

    Plasmid

    #19955

    Depositor

    Yue Xiong

    Article

    Shumway et al J Biol Chem. 2003 Jan 24. 278(4):2089-92

    Insert

    Tsc1 (Tuberous sclerosis 1) (TSC1 Human)
    

    Use

    Tags

    Myc3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2009

    Availability

    Academic Institutions and Nonprofits only

38. pcDNA5/FRT/TO HSPA1L

    Plasmid

    #19457

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    HSPA1L (HSPA1L Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3.1_C-6His-Stx17

    Plasmid

    #112259

    Purpose

    C-term His tagged pcDNA3.1 vector expressing mouse Stx17 (syntaxin 17)

    Depositor

    Madhulika Tripathi

    Insert

    syntaxin 17 (Stx17 Mouse)
    

    Use

    Tags

    6His Tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3 Flag Fli1

    Plasmid

    #66976

    Purpose

    Expression plasmid for mouse Fli1; adds Flag tag to the N-terminus of protein

    Depositor

    Christopher Vakoc

    Article

    Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

    Insert

    Fli1 (Fli1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only