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We narrowed to 6,273 results for: nls

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Showing: 3001 - 3050 of 6273 results

  3. BPK3258 - human expression plasmid for eSpCas9(1.1)

    Plasmid
    #101176
    Purpose
    Human expression plasmid for SpCas9 eSpCas9(1.1) variant: CMV-T7-hSpCas9-eSpCas9(1.1)(K848A, K1003A, R1060A)-NLS(SV40)-3xFLAG
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-eSpCas9(1.1)(K848A/K1003A/R1060A)
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    K848A/K1003A/R1060A
    Promoter
    CMV
    Available Since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101176-plasmid-map-sequence-id-193999

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  4. VanGlow-GL-DSCP

    Plasmid
    #83338
    Purpose
    for Gateway cloning of enhancer elements upstream of the DSCP and GFP::Luciferase(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.
    Depositor
    Cheng-Yu Lee
    Article
    Janssens et al Dev Cell. 2017 Feb 27;40(4):367-380.e7.
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    GFP::Luciferase(nls)
    Expression
    Insect
    Promoter
    Drosophila Synthetic Core Promoter
    Available Since
    Dec. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    83338-plasmid-map-sequence-id-158645

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  12. Myopathy Loop Mutant Nuclear Myosin I YFP

    Plasmid
    #60243
    Purpose
    Expresses a myopathy loop mutant (6 mutations) of the nuclear isoform of myosin IC attached to YFP and an NLS, which has impaired actin binding activity
    Depositor
    Primal de Lanerolle
    Article
    Chuang et al Curr Biol. 2006 Apr 18;16(8):825-31.
    Insert
    Nuclear Myosin 1 (Myo1c Mouse)
    Tags
    EYFP and NLS
    Expression
    Mammalian
    Mutation
    Deleted I339, I340, G343, E344, E345,L346
    Promoter
    CMV
    Available Since
    Dec. 2, 2014
    Availability
    Academic Institutions and Nonprofits only

  15. pCMV dCas9-MC (eGFP)

    Plasmid
    #89931
    Purpose
    Expresses the dCas9-MC fragment only of the sMTase system for targeted DNA methylation in mammalian cells. Contains a eGFP marker expressed off separate promoter.
    Depositor
    Carl Novina
    Article
    Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.
    Insert
    dCas9-MC
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    deactivated Cas9, fragment of M.SssI (residues 27…
    Promoter
    CMV
    Available Since
    Sept. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    89931-plasmid-map-sequence-id-177995

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  19. VRER variant

    Plasmid
    #138561
    Purpose
    Expresses human codon-optimized SpCas9-VRER and blasticidin resistance: EFS promoter-VRER-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    VRER
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    D1135V, G1218R, R1335E, T1337R
    Promoter
    EFS
    Available Since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138561-plasmid-map-sequence-id-274783

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  34. iPEmax-WT

    Plasmid
    #239385
    Purpose
    For inverse prime editor using Cas9-R221K-N394K in HEK294T cells
    Depositor
    Caixia Gao
    Article
    Liang et al Nat Commun. 2025 May 31;16(1):5057. doi:
    Insert
    Cas9-R221K-N393K, M-MLV-RT
    Use
    CRISPR
    Tags
    BPNLS and SV40 NLS, c-myc NLS
    Expression
    Mammalian
    Mutation
    R221K, N394K in Cas9
    Promoter
    CMV
    Available Since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    239385-plasmid-map-sequence-id-481781

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  35. iPEmax-D10A

    Plasmid
    #239386
    Purpose
    For inverse prime editor using nCas9-D10A-R221K-N394K in HEK295T cells
    Depositor
    Caixia Gao
    Article
    Liang et al Nat Commun. 2025 May 31;16(1):5057. doi:
    Insert
    nCas9-D10A-R221K-N394K, M-MLV-RT
    Use
    CRISPR
    Tags
    BPNLS and SV40 NLS, c-myc NLS
    Expression
    Mammalian
    Mutation
    D10A, R221K, N394K in Cas9
    Promoter
    CMV
    Available Since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    239386-plasmid-map-sequence-id-481780

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  42. pPB9v2

    Plasmid
    #210029
    Purpose
    Contains cloning site for molecularly-tagged Cas9 gRNA constructs, inducible Cas9, rtTA-T2A-puro
    Depositor
    Michael Talkowski
    Article
    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi
    Inserts
    Cas9
    rtta-T2A-puro
    Use
    CRISPR
    Tags
    SV40 NLS and nucleoplasmin NLS
    Expression
    Mammalian
    Promoter
    EF-1α and TRE3G
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    210029-plasmid-map-sequence-id-414001

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  44. SpCas9-NRCH-CGBE1

    Plasmid
    #198557
    Purpose
    Expresses human codon-optimized SpCas9-NRCH-CGBE1 and blasticidin resistance: EFS promoter-SpCas9-NRCH-CGBE1-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NRCH-CGBE1
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198557-plasmid-map-sequence-id-388917

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Showing: 3001 - 3050 of 6273 results