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AAV-EF1a-FLEx-ASAP4b-Kv-WPRE

Plasmid

#201032

Purpose

Expresses somatically enriched ASAP4b from the EF-1alpha promoter in a cre-dependent manner

Depositor

Michael Lin

Article

Evans et al Nat Methods. 2023 Jul;20(7):1104-1113. d

Insert

ASAP4e-Kv

Use

AAV

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pFastBac_Dual-GST-TEV-SINTBAD-EGFP (codon optimized Sf9)

Plasmid

#198035

Purpose

Used for the expression and purification of EGFP labelled SINTBAD (SMC Internal No. 1613)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

SINTBAD (TBKBP1 Human)

Tags

EGFP and GST-TEV

Expression

Insect

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO {mCAR}off-{GCaMP6f}on-WPRE

Plasmid

#111393

Purpose

AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection) and Cre-ON GCaMP6f (ultrasensitive calcium sensor)

Depositor

Adam Kepecs

Article

Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

Inserts

mCAR (Cxadr Mouse)
GCaMP6f

Use

AAV

Tags

6xHis, Myc, T7, and Xpress

Expression

Mammalian

Promoter

hSynapsin

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pETDuet-1_6xHis-TEV-mCh-NDP52

Plasmid

#187829

Purpose

Plasmid for the expression and purification of 6xHis-TEV-mCh-NDP52 in E. coli Rosetta pLysS. Internal Reference: SMC1148

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

NDP52 (CALCOCO2 Human)

Tags

6xHisTag, TEV, and mCherry

Expression

Bacterial, Insect, and Mamm…

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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FLAG-β-arrestin2 RRK/Q-5-kinase domain fusion protein (RRK5K)

Plasmid

#38262

Depositor

Robert Lefkowitz

Article

Nelson et al J Biol Chem. 2008 Jul 25;283(30):21093-1

Insert

β-arrestin2 (Arrb2 Rat)

Tags

Flag and PIP5K Iα core kinase domain (residues 18…

Expression

Mammalian

Mutation

Arg 233, Arg237, and Lys251 converted to Glutamin…

Promoter

CMV

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pMMK ENTR 4 CMV GST mTagBFP

Plasmid

#206259

Purpose

ENTR Vector 4 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mTagBFP localised to Golgi organelles under the control of CMV promoter.

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

GTS mTagBFP

Use

Multimate/gateway entr 4

Tags

BFGT1 (Golgi localisation peptide)

Expression

Mammalian

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mVenus1-155-I152L-GGGS

Plasmid

#162613

Purpose

Allows for transcription of improved control mVenus I152L fluorophore half for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

mVenus

Expression

Mammalian

Mutation

1-155-I152L

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO {mCAR}off-{ChETA}on-WPRE

Plasmid

#111387

Purpose

AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection) and Cre-ON ChETA (for optogenetic activation)

Depositor

Adam Kepecs

Article

Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

Inserts

mCAR (Cxadr Mouse)
ChETA

Use

AAV

Tags

HA and Myc

Expression

Mammalian

Promoter

hSynapsin

Available Since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-BRAF-WT-IRES-mCherry

Plasmid

#221027

Purpose

Fluorescent reporter for expressing a segment of wild type BRAF

Depositor

Mohamed Fareh

Article

Shembrey et al Sci Adv. 2024 Dec 20;10(51):eadl0731. do

Insert

BRAF wildtype (BRAF Human)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GABARAP(G116A)-mTagBFP2- GABARAP

Plasmid

#212106

Purpose

Expression of GABARAP split tandem protein with mTagBFP2

Depositor

Dieter Willbold

Article

Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

Insert

GABARAP(G116A)-mTagBFP2-GABARAP (GABARAP Human, Entacmaea quadricolor)

Tags

mTagBFP2

Expression

Mammalian

Mutation

G116A (lipidation deficiency) of upstream GABARAP

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GABARAP(G116A)-mTagBFP2-GABARAP(G116A)

Plasmid

#212108

Purpose

Expression of lipidation deficient GABARAP split tandem protein with mTagBFP2

Depositor

Dieter Willbold

Article

Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

Insert

GABARAP(G116A)-mTagBFP2-GABARAP(G116A) (GABARAP Human, Entacmaea quadricolor)

Tags

mTagBFP2

Expression

Mammalian

Mutation

G116A (lipidation deficiency) of both GABARAPs

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pT3TS-zSpG

Plasmid

#179316

Purpose

In vitro transcription of SpG-Cas9 mRNA zebrafish codon-optimized from T3 promoter

Depositor

Miguel Angel Moreno-Mateos

Article

Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

Insert

Zebrafish codon-optimized Cas9 variant SpG

Use

CRISPR; In vitro transcription by t3 promoter

Tags

SV40-NLS

Mutation

SpG=D1135L/S1136W/G1218K/E1219Q/ R1335Q/T1337R

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pT3TS-zSpRY

Plasmid

#179317

Purpose

In vitro transcription of SpRY-Cas9 mRNA zebrafish codon-optimized from T3 promoter

Depositor

Miguel Angel Moreno-Mateos

Article

Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

Insert

Zebrafish codon-optimized Cas9 variant SpRY

Use

CRISPR; In vitro transcription by t3 promoter

Tags

SV40-NLS

Mutation

SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/ N13…

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-endo-GsCT

Plasmid

#205516

Purpose

Endosome-targeted C-terminal fragment of human Gαs protein (Gαs inhibitory peptide).

Depositor

Jin Zhang

Article

Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

Insert

endo-GsCT (GNAS Human, Synthetic)

Tags

2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pK029.CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE (Supernova)

Plasmid

#69138

Purpose

For the Supernova, a series of vector systems for single-cell labeling and gene function sudies in vivo

Depositor

Takuji Iwasato

Article

Mizuno et al Neuron. 2014 Apr 16;82(2):365-79. doi: 1

Insert

CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE

Tags

none

Expression

Mammalian

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-nuc-GsCT

Plasmid

#205517

Purpose

Nuclear-targeted C-terminal fragment of human Gαs protein (Gαs inhibitory peptide).

Depositor

Jin Zhang

Article

Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

Insert

nuc-GsCT (GNAS Human, Synthetic)

Tags

Histone H2A and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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AID-BE3

Plasmid

#100803

Purpose

Expresses AID-BE3 in mammalian cells

Depositor

David Liu

Article

Komor et al Sci Adv. 2017 Aug 30;3(8):eaao4774. doi:

Insert

activation induced cytidine deaminase (AICDA Human, S. pyogenes)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pET28b-SpG-His

Plasmid

#179318

Purpose

Plasmid for bacterial expression and purification of SpG-Cas9

Depositor

Julián Cerón, Miguel Angel Moreno-Mateos

Article

Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

Insert

Human codon-optimized Cas9 variant SpG

Use

CRISPR

Tags

6xHis and SV40-NLS

Expression

Bacterial

Mutation

SpG=D1135L/S1136W/G1218K/E1219Q/ R1335Q/T1337R

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO {mCAR}off-{DTR-GFP}on-WPRE

Plasmid

#111395

Purpose

AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection), Cre-ON DTR-EGFP (diphtheria toxin receptor for cell ablation)

Depositor

Adam Kepecs

Article

Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

Inserts

mCAR (Cxadr Mouse)
DTR-EGFP

Use

AAV

Tags

EGFP and Myc

Expression

Mammalian

Promoter

hSynapsin

Available Since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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MIG-eIF4A1 P159E

Plasmid

#70042

Purpose

Expression of eIF4A1 P159E mutant construct in mammalian cells.

Depositor

Hans-Guido Wendel

Article

Wolfe et al Nature. 2014 Sep 4;513(7516):65-70. doi:

Insert

eIF4A1 (Eif4a1 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

P159E

Promoter

CMV

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only