We narrowed to 2,342 results for: clu

1. pCMV-pro-Il1b-C-Flag

   Plasmid

   #75131

   Purpose

   Expresses C-terminal flag-tagged pro-IL-1β in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   interleukin 1 beta (Il1b Mouse)
   

   Use

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   (please see depositor comments below)

   Promoter

   CMV

   Available since

   Sept. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmEos2-N1 PH-PLCd (wild-type) (PH-PLCd-mEos2)

   Plasmid

   #162877

   Purpose

   Expression in mammalian cells of Plekstrin-homology domain of Phospholipase C delta tagged with mEos2 to perform sptPALM

   Depositor

   Frederic Meunier

   Article

   Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

   Insert

   pleckstrin homology domain of PLCδ1 (phospholipase C-δ1) (PLCD1 Human)
   

   Use

   Tags

   mEos2

   Expression

   Mammalian

   Mutation

   Insert consists of AA1-170

   Promoter

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-α-cateninA-

   Plasmid

   #229698

   Purpose

   Transient expression of GFP-alpha-cateninA- in mammalian cells

   Depositor

   Alpha Yap

   Article

   Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

   Insert

   CTNNA1 (alpha-catenin) Human (CTNNA1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   L785A, I792A, V796A

   Promoter

   CMV promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. TRE-TDP-43ΔNLS-mClover3

   Plasmid

   #214916

   Purpose

   inducible expression of TDP-43 harboring mutant NLS and C-terminal mClover3. Expression yields cytosolic diffuse TDP-43

   Depositor

   Ophir Shalem

   Article

   Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

   Insert

   TDP-43ΔNLS (TARDBP Human)
   

   Use

   Lentiviral

   Tags

   mClover3

   Expression

   Mutation

   mutant NLS GCAGCAGCAATGGATGAGACAGATGCTTCATCAGCAGT…

   Promoter

   Available since

   May 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-C1 PA-PLA1 (EGFP-PA-PLA1)

   Plasmid

   #162880

   Purpose

   Expression in mammalian cells of Phosphatidic Acid preferring Phospholipase A1 tagged with EGFP

   Depositor

   Frederic Meunier

   Article

   Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

   Insert

   Phosphatidic acid-preferring phospholipase A1 (PA-PLA1) (DDHD1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-α-cateninΔβH

   Plasmid

   #229705

   Purpose

   Transient expression of GFP-alpha-catenin-delta-beta-H in mammalian cells

   Depositor

   Alpha Yap

   Article

   Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

   Insert

   CTNNA1 (alpha-catenin) Human (CTNNA1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. GFP-α-cateninA+

   Plasmid

   #229697

   Purpose

   Transient expression of GFP-alpha-cateninA+ in mammalian cells; enhanced actin binding

   Depositor

   Alpha Yap

   Article

   Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

   Insert

   CTNNA1 (alpha-catenin) Human (CTNNA1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   amino acids 670-673, RAIM--> GSGS

   Promoter

   CMV promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-α-cateninA+ΔβH

   Plasmid

   #229704

   Purpose

   Transient expression of GFP-alpha-cateninA+deltabetaH in mammalian cells

   Depositor

   Alpha Yap

   Article

   Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

   Insert

   CTNNA1 (alpha-catenin) Human (CTNNA1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   amino acids 670-673, RAIM--> GSGS

   Promoter

   CMV promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pK27Sumo_His-SUMO-nsp10-nsp14 fusion (SARS-CoV-2)

   Plasmid

   #169161

   Purpose

   To express nsp10-nsp14 fusion protein in E. coli

   Depositor

   John Diffley

   Article

   Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

   Insert

   14His-SUMO-nsp10-GGSGGS-nsp14 (ORF1ab Synthetic, SARS-CoV-2)
   

   Use

   Tags

   14His-SUMO and Fusion protein of SARS-CoV-2 nsp10…

   Expression

   Bacterial

   Mutation

   Codon optimised for E. coli

   Promoter

   T5

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. lucMAPT-30D

    Plasmid

    #214672

    Purpose

    MAPT Exon 10 reporter containing the MS2 hairpin 30 base pairs downstream of the 5′ splice site

    Depositor

    Eugene Yeo

    Article

    Schmok et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    MAPT (MAPT Human)
    

    Use

    Tags

    Firefly Luciferase, Renilla Luciferase, and V5

    Expression

    Mammalian

    Mutation

    Contains Exon 9, Exon 10 with a mutation to intro…

    Promoter

    CMV

    Available since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. lucMAPT-30U

    Plasmid

    #214673

    Purpose

    MAPT Exon 10 reporter containing the MS2 hairpin 30 base pairs upstream of the 3′ splice site

    Depositor

    Eugene Yeo

    Article

    Schmok et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    MAPT (MAPT Human)
    

    Use

    Tags

    Firefly Luciferase, Renilla Luciferase, and V5

    Expression

    Mammalian

    Mutation

    Contains Exon 9, Exon 10 with a mutation to intro…

    Promoter

    CMV

    Available since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLViN-iRFP670-α-cateninWT

    Plasmid

    #229702

    Purpose

    Lentiviral expression of iRFP670-alpha-cateninWT in mammalian cells

    Depositor

    Alpha Yap

    Article

    Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

    Insert

    CTNNA1 (alpha-catenin) Human (CTNNA1 Human)
    

    Use

    Lentiviral

    Tags

    iRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. dCas9-p300 HAT

    Plasmid

    #179544

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT domain (aa 1287-1664) driven by AF4 promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. pyogenes dCas9 with c-terminal fusion of human p300 HAT domain (aa 1287-1664) (EP300 Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRSFDuet-FBXL5-SKP1

    Plasmid

    #226620

    Purpose

    Co-expression of FBXL5-SKP1 complex in E.coli

    Depositor

    Ning Zheng

    Article

    Wang et al Mol Cell. 2020 Apr 2;78(1):31-41.e5. doi

    Inserts

    FBXL5 (FBXL5 Human)
    SKP1 (SKP1 Human)
    

    Use

    Tags

    GB1

    Expression

    Bacterial

    Mutation

    Amino acids 1-198 was deleted, and 420-596 was re…

    Promoter

    T7

    Available since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLViN-iRFP670-α-cateninA+

    Plasmid

    #229703

    Purpose

    Lentiviral expression of iRFP670-alpha-cateninA+ in mammalian cells

    Depositor

    Alpha Yap

    Article

    Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

    Insert

    CTNNA1 (alpha-catenin) Human (CTNNA1 Human)
    

    Use

    Lentiviral

    Tags

    iRFP670

    Expression

    Mammalian

    Mutation

    amino acids 670-673, RAIM--> GSGS

    Promoter

    CMV promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pK27Sumo_His-SUMO-nsp14-nsp10 fusion (SARS-CoV-2)

    Plasmid

    #169160

    Purpose

    To express nsp14-nsp10 fusion protein in E. coli

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    14His-SUMO-nsp14-GGSGGS-nsp10 (ORF1ab Synthetic, SARS-CoV-2)
    

    Use

    Tags

    14His-SUMO and Fusion protein of SARS-CoV-2 nsp14…

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDEST-Myc-FBW7 Delta F-Box

    Plasmid

    #197452

    Purpose

    The plasmid expresses F-box deleted version of Myc-tagged FBW7 human isoform 1. Used for mammalian overexpression of FBW7alpha delta-F-Box and detection by western blotting.

    Depositor

    Omar Khan

    Article

    Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:

    Insert

    FBW7 (FBXW7 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    FBW7 Fbox deleted.

    Promoter

    CMV

    Available since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. DNA-CARzeta-GFP

    Plasmid

    #89344

    Purpose

    2nd generation lentiviral vector which expresses DNA-CAR receptor fused to GFP

    Depositor

    Ron Vale

    Article

    Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do

    Insert

    DNA-CARzeta (CD247, CD86 Human)
    

    Use

    Lentiviral

    Tags

    SNAPf tag and mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEMS1951

    Plasmid

    #105868

    Purpose

    High copy number plasmid containing Hspa1a (synonym Hsp68) MiniPromoter insert

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

    Insert

    Hspa1a (synonym Hsp68) MiniPromoter (HSPA1A, HSPA1A Human)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Hsp68 MiniPromoter

    Available since

    Feb. 22, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pmEos2-N1 GBP (GBP-mEos2)

    Plasmid

    #162876

    Purpose

    Expression in mammalian cells of GFP binding protein (GBP) tagged with photoconvertable protein mEos2 to track GFP proteins of interest to perform Fluorescent intrabody Localization Microscopy

    Depositor

    Frederic Meunier

    Article

    Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

    Insert

    GFP Binding Protein tagged with mEos2
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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