We narrowed to 41,233 results for: ids
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Plasmid#180613PurposeBacterial expression for CALPAIN-7 MIT domains, residues 1-165. Has F98D mutation. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-27.DepositorInsertCALPAIN-7
TagsHIS-SUMOExpressionBacterialMutationResidues 1-165, F98D mutationPromoterT7Available SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pgRNA_CV2
Plasmid#167165PurposepgRNA_CV2 is derived from gRNA_cloning vector (Addgene plasmid ID: 41824) by adding about 80 bps from the sgRNA sequence as well as an AgeI site. In the literature, sgRNA_AL is used as an alias.DepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterU6 promoterAvailable SinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
Sema4f.a-Fc-His
Plasmid#72158PurposeExpresses the extracellular region of the Sema4F, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGRG36-Kn-PA1-GFP
Plasmid#79088PurposepGRG36-Kn-PA1-GFP is a Kanamycine resistance plasmid used to integrate a GFP expression cassette into the chromosome of E.coli, described in Yang et al. 2016 (Pending publication Pubmed ID)DepositorInsertGFP
UseBacterial chromosome integration and expression o…ExpressionBacterialPromoterPA1Available SinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBBR15.2-2H12.D11opt-scarRef
Plasmid#221150PurposecdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11241)DepositorInsertscdGreen2.1
mScarlet-I
ExpressionBacterialMutationN-terminus changed: starts with MSKKYGEAVIKE ...PromoterConstitutive and None (same as cdGreen2.1)Available SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSP6-RNAP
Plasmid#48143PurposeShuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage SP6 under control of the xylose inducible promoter PxylADepositorInsertrna polymerase SP6
UseSynthetic BiologyExpressionBacterialMutationV314A (numbering based on NCBI Sequence ID: NP_85…Available SinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTT3 Unc5C-FLAG
Plasmid#72196PurposeExpresses full-length Unc5c with a C-terminal FLAG tagDepositorAvailable SinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
GST-CHMP4A (1-204) (pGEX2T(TEV))
Plasmid#80632PurposeResidues 1-204 of CHMP4A; Bacterial Expression vector; TEV-cleavable GST tag; Sundquist Lab internal ID: WISP06-198DepositorAvailable SinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ntn1-Fc-His
Plasmid#72104PurposeExpresses the entire Netrin 1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEM-Cas9HF1-recA56
Plasmid#89962PurposeModified from pEM-Cas9HF1 (Addgene ID: 89961) to include constitutive recA56 to block recA-mediated double-strand break repair.DepositorInsertsCas9HF1
recA56
ExpressionBacterialMutationN497A/R661A/Q695A/Q926APromoterpTet and proDAvailable SinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
Plxna1-Fc-His
Plasmid#72122PurposeExpresses the extracellular region of the PlexinA1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceMarch 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
Plxna1-AP-His
Plasmid#71996PurposeExpresses the extracellular region of the PlexinA1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
Unc5b.z-AP-His
Plasmid#72053PurposeExpresses the extracellular region of the Unc5B, isoform z protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
GFP-BLM
Plasmid#80070PurposeExpresses GFP-tagged BLM helicase in mammalian cells (EGFP-C1 backbone)DepositorInsertBLM (BLM Human)
TagsGFPExpressionMammalianMutationIf correct ID, the cDNA is from HeLa and has char…Available SinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-OSF-KATNA1
Plasmid#160051PurposeMammalian expression vector for KATNA1 (KATANIN-P60) with N-terminal One-Strep-Flag tag. Internal ID: WISP20-18.DepositorAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTT3 Unc5B-FLAG
Plasmid#72195PurposeExpresses full-length Unc5b with a C-terminal FLAG tagDepositorAvailable SinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBtPIPLC His
Plasmid#173808PurposeExpression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cellsDepositorInsertBacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
Tags6XHisExpressionBacterialMutationnonePromoterT7Available SinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
Plxna4-Fc-His
Plasmid#72125PurposeExpresses the extracellular region of the PlexinA4 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceMarch 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEM-Cas9HF1-indRecA56
Plasmid#102294PurposeModified from pEM-Cas9HF1 (Addgene ID: 89961) to co-express inducible recA56 to block recA-mediated double-strand break repair.DepositorInsertsCas9HF1
recA56
UseCRISPRExpressionBacterialMutationN497A/R661A/Q695A/Q926APromoterpTetAvailable SinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTT3 Unc5D-FLAG
Plasmid#72197PurposeExpresses full-length Unc5d with a C-terminal FLAG tagDepositorAvailable SinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only