We narrowed to 78,412 results for: rest
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Plasmid#192987PurposePlasmid to test the Hydra Wnt3 promoter activity (full deletion of the repressor element)DepositorInsertHydra Wnt3 promoter
ExpressionMammalianMutationRepressor element deletedAvailable SinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-Swa1::HA-RfA
Plasmid#186404PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter HA tag and a gene of interest under control of a reg. sequence in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-Swa1::RfA-ECFP
Plasmid#186402PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter eCFP under control of a reg. sequence in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-Swa1::venus-RfA
Plasmid#186398PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter Venus and a gene of interest under control of a reg. sequence in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-Swa1::RfA-HA
Plasmid#186405PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter HA tag under control of a reg. sequence in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX459-HypaCas9-CfANLN_sgRNA
Plasmid#183880PurposepX459V2.0-HypaCas9 plasmid with cfANLN sgRNA for N-terminal tagging of anillin in canine (Canis familiaris) cells.DepositorInsertCanis familiaris ANLN sgRNA spacer
UseCRISPRExpressionMammalianPromoterU6Available SinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTrichoGate-17
Plasmid#160717PurposeTrpC Terminator bordered by BsaI restriction sitesDepositorInserttryptophan synthase transcription terminator (TtrpC) bordered by BsaI restriction sites
UseSynthetic BiologyMutationIncorporation of BsaI restriction sitesAvailable SinceMarch 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
mTurquoise2-RnBarr2
Plasmid#137798PurposeVisualization of beta-arrestin2DepositorAvailable SinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Rem2 S69A/S241A/S308A/S334A
Plasmid#51594Purposeexpresses RNAiR myc-tagged Rem2 S-A mutantDepositorInsertRem2 (Rem2 Mouse)
TagsmycExpressionMammalianMutationSerines 69, 241, 308, 334 to Alanines; also RNAi …PromoterCMVAvailable SinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti-sgCTRL-1
Plasmid#83934PurposeLentiviral vector expressing a control sgRNA. NOTE: This plasmid does NOT contain Cas9.DepositorInsertsgCTRL-1
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO Dual-Luciferase-PDAP1-3'UTR
Plasmid#182251PurposeHuman PDAP1 3` UTR region containing 4x binding sites for miR-150 cloned downstream of luciferase reporter geneDepositorInsert3' UTR region of PDAP1 gene
UseLuciferaseExpressionMammalianAvailable SinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSGV-1D3-28Z All ITAMs intact
Plasmid#107226PurposeAnti-mouse CD19 CAR with CD3 ITAMs intactDepositorInsert1D3-28Z All ITAMs intact
UseRetroviralTagsNoneMutationNoneAvailable SinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFLAG-Tetherin
Plasmid#41070DepositorAvailable SinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
MSGV1-1D3-28Z.1-3 mut
Plasmid#107227PurposeAnti-mouse CD19 CAR with CD3 ITAMs mutatedDepositorInsert1D3-28Z. 1-3 mut
UseRetroviralTagsNoneMutationNoneAvailable SinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
1098 pcDNA GFP FKHR
Plasmid#9022DepositorAvailable SinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
mRFP-Rab7
Plasmid#14436PurposeMammalian expression of Rab7 fused to mRFP. Used for localization to the late endosome, and other early endosomal vacuoles.DepositorInsertRab 7 (RAB7A Canis familialis)
TagsmRFPExpressionMammalianMutation3 silent mutations near the C-terminusAvailable SinceMarch 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TetOne-Puro-LUC
Plasmid#218630PurposeThis plasmid contains luciferase under doxycycline inducible control.DepositorAvailable SinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
(133) pcDNA3.1-Nup62-Flag-EPEA
Plasmid#160744PurposeExpresses the nucleoporin Nup62 with C-terminal Flag and EPEA tags. Proteins of interest can be inserted by replacing Nup62 using Sgfl + Sgsl restriction enzymesDepositorInsertNup62-Flag-EPEA
TagsEPEA and FlagExpressionMammalianPromoterT7/CMVAvailable SinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hRREB1-RFP
Plasmid#22924DepositorInserthuman ras responsive element binding protein 1 driving DsRedExpress (RREB1 Human)
UseAAVExpressionMammalianAvailable SinceMay 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
PB-UniSAM
Plasmid#99866PurposeEncodes for Cas9-VP64, MS2-p65-HSF1, mCherry and for the gRNA 2.0DepositorInsertUniSAM-mCherry + U6-gRNA2.0
UseCRISPR and Synthetic Biology; Dcas9-sam activationExpressionMammalianMutationBbsI sites in CDS were ablated by consensus mutat…PromoterEF1aAvailable SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only