8,807 results

1. Slug_pGL2

   Plasmid

   #31695

   Depositor

   Paul Wade

   Article

   Fujita et al Cell. 2003 Apr 18. 113(2):207-19.

   Insert

   Slug (SNAI2 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-EF1a-FLEX-GTB

   Plasmid

   #26197

   Depositor

   Edward Callaway

   Article

   Haubensak et al Nature. 2010 Nov 11. 468(7321):270-6.

   Insert

   Enhanced green fluorescent protein, TVA, rabies B19 glycoprotein
   

   Use

   AAV and Cre/Lox; Adeno-associated virus

   Mutation

   TVA, rabies glycoprotein are mammalian codon opti…

   Available Since

   Nov. 11, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. mito-BFP

   Plasmid

   #49151

   Purpose

   visualization of mitochondria

   Depositor

   Gia Voeltz

   Article

   Friedman et al Science. 2011 Oct 21;334(6054):358-62. d

   Insert

   mitochondrial targeting signal (COX4 Budding Yeast)
   

   Tags

   BFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. LSL-Cas9-Rosa26TV

   Plasmid

   #61408

   Purpose

   Cre-dependent Cas9 expression plasmid and targeting vector for the mouse Rosa26 locus. Cas9 expression is Cre-dependent and the targeting vector has positive (Neo) and negative (DTA) selection.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   Cas9
   EGFP
   

   Use

   CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   3xFLAG and P2A

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   CAG

   Available Since

   Jan. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. 1036 pcDNA3 Myr HA Akt1

   Plasmid

   #9008

   Purpose

   Mammalian expression of human Akt1 with myr, HA tags

   Depositor

   William Sellers

   Article

   Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

   Insert

   Akt1 (AKT1 Human)
   

   Tags

   HA and myr

   Expression

   Mammalian

   Available Since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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6. Str-Ii_VSVG-SBP-mCherry

   Plasmid

   #65301

   Purpose

   synchronize trafficking of VSVG from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   VSV-G
   

   Tags

   Streptavidin Binding Protein (SBP) and mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHCMV-EcoEnv

   Plasmid

   #15802

   Depositor

   Miguel Sena-Esteves

   Article

   Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

   Insert

   MLV env (ecotropic)
   

   Expression

   Mammalian

   Available Since

   March 21, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBMN(CMV-copGFP-Puro-SMAR)

   Plasmid

   #80391

   Purpose

   expression copGFP and Puromycine, with an S/MAR element for episomal maintaince

   Depositor

   Magnus Essand

   Article

   Jin et al EMBO Mol Med. 2016 May 6. pii: e20150586

   Insert

   copGFP-T2A-Puromycine
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. 3XFlagNICD2

   Plasmid

   #20184

   Depositor

   Raphael Kopan

   Article

   Ong et al J Biol Chem. 2006 Feb 24. 281(8):5106-19

   Insert

   Notch 2 intracellular domain (Notch2 Mouse)
   

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   Contains murine Notch 2 Intracellular Domain (Met…

   Promoter

   CMV

   Available Since

   Jan. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pZR112_Lenti-SFFV-mCherry-2A-dCas9-VP64

    Plasmid

    #180263

    Purpose

    Lentiviral expression vector for dCas9-VP64 in mammalian cells

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    Nuclease dead Cas9

    Promoter

    SFFV

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA 3.1(Hygro) myc-hPolQ-Flag

    Plasmid

    #73132

    Purpose

    To express in mammalian cells human PolQ

    Depositor

    Agnel Sfeir

    Article

    Mateos-Gomez et al Nature. 2015 Feb 12;518(7538):254-7. doi

    Insert

    Polymerase theta (POLQ Human)
    

    Tags

    Flag and myc

    Expression

    Mammalian

    Promoter

    cmv promoter

    Available Since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. lenti-sgRNA blast

    Plasmid

    #104993

    Purpose

    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and blasticidin S resistance from an EF-1a promoter.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. EGFP-alphasynuclein-A53T

    Plasmid

    #40823

    Depositor

    David Rubinsztein

    Article

    Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

    Insert

    SNCA (SNCA Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    A53T (aggregates more rapidly than the wild-type …

    Promoter

    CMV

    Available Since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. ER-GCaMP6f

    Plasmid

    #182548

    Purpose

    Expresses GCaMP6f on the cytosolic side of the ER (P-450site)

    Depositor

    Chris Richards

    Article

    Aryal et al Anal Chem. 2022 Jan 21. doi: 10.1021/acs

    Insert

    ER localized P450
    

    Expression

    Bacterial and Mammalian

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Flag-hPIASx beta

    Plasmid

    #15210

    Depositor

    Ke Shuai

    Article

    Arora et al J Biol Chem. 2003 Jun 13. 278(24):21327-

    Insert

    PIASx beta (PIAS2 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-GFP

    Plasmid

    #11150

    Purpose

    Mammalian expression vector for expression of GFP (CAG promoter)

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

    Has Service

    Cloning Grade DNA

    Insert

    EGFP from Aequorea victoria
    

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBAD33.1

    Plasmid

    #36267

    Depositor

    Christian Raetz

    Article

    Chung et al Biochemistry. 2010 May 18;49(19):4126-37

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    PBAD

    Available Since

    Oct. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. Flag-hPIASy

    Plasmid

    #15208

    Depositor

    Ke Shuai

    Article

    Liu et al Proc Natl Acad Sci U S A. 2001 Mar 13. 9

    Insert

    PIASy (PIAS4 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP

    Plasmid

    #60903

    Purpose

    Expressed a nuclease dead Cas9 tagged with 10 copies of the GCN4 peptide v4 and BFP. This plasmid is part of the SunTag system for gene activation

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    Cas9 dead
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-ChrimsonR-tdT

    Plasmid

    #59169

    Purpose

    Expression vector for ChrimsonR-tdTomato

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR-tdTomato
    

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    K176R

    Promoter

    CAG

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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