Addgene

We narrowed to 6,032 results for: WUS;

Showing: 1831 - 1860 of 6032 results

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pYPQ142-ZmUbi-tRNA

Plasmid

#158578

Purpose

Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 and pYPQ132-tRNA2.0; assembly of 2 gRNAs

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Maize ubiquitin 1

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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sgLenti

Plasmid

#105996

Purpose

single sgRNA expression vector

Depositor

Mike McManus

Article

Boettcher et al Nat Biotechnol. 2018 Jan 15. pii: nbt.40

Type

Empty backbone

Use

CRISPR and Lentiviral; S.pyogenes sgrna expressio…

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-mTurquoise2

Plasmid

#99125

Purpose

An AAV genome that expresses the fluorescent protein mTurquoise2 from the hSyn1 promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mTurquoise2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn1

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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Human IgM-Fc

Plasmid

#158214

Purpose

Express the Fc region of human IgM

Depositor

Junyu Xiao

Article

Li et al Science. 2020 Feb 28;367(6481):1014-1017

Insert

IGHM (IGHM Human)

Use

Tags

twin strep

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-mNeonGreen

Plasmid

#99135

Purpose

An AAV genome that ubiquitously expresses the fluorescent protein mNeonGreen from the hSyn1 promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mNeonGreen

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn1

Available since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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mEos3.2-N1

Plasmid

#54525

Purpose

Localization: N1 Cloning Vector, Excitation: 507 / 572, Emission: 516 / 580

Depositor

Tao Xu, Michael Davidson

Article

Zhang et al Nat Methods. 2012 May 13;9(7):727-9. doi

Type

Empty backbone

Use

Tags

mEos3.2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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Dnase1L3 pIRES-DsRed

Plasmid

#98845

Purpose

Expresses Human Dnase1L3 in mammalian cells along with DsRed

Depositor

Peter Keyel

Article

Shi et al Front Immunol. 2017 May 8;8:522. doi: 10

Insert

Dnase1L3 (DNASE1L3 Human)

Use

Tags

IRES DsRed

Expression

Mammalian

Mutation

long isoform, wild type

Promoter

CMV

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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mEos3.2-C1

Plasmid

#54550

Purpose

Localization: C1 Cloning Vector, Excitation: 507 / 572, Emission: 516 / 580

Depositor

Tao Xu, Michael Davidson

Article

Zhang et al Nat Methods. 2012 May 13;9(7):727-9. doi

Type

Empty backbone

Use

Tags

mEos3.2

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pCambia-PUP-IT

Plasmid

#186478

Purpose

Expresses POI-PUP-IT fusion in Plants

Depositor

Jen Sheen

Article

Ye et al Nature. 2022 Sep 14. pii: 10.1038/s41586

Insert

FLAG-Pup(E)

Use

Tags

Triple Flag Tag

Expression

Plant

Mutation

Promoter

35S

Available since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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UBQ10:sXVE:(MCS)-S11-DI-GFP1–9

Plasmid

#108260

Purpose

Binary vectors used for the inducible expression of dual intein-coupled tripartite split-sfGFP components in plants

Depositor

Tzu-Yin Liu

Article

Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

UBQ10:sXVE:

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTorPE-jREX-GECO1

Plasmid

#113941

Purpose

Bacterial expression of red fluorescent Ca2+ sensor jREX-GECO1

Depositor

Thomas Hughes, Robert Campbell

Article

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

jREX-GECO1

Use

Tags

6xHis and Modified TorA MNNNDLFQASRRRFLAQLG[G to …

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pDualIN-SMN2

Plasmid

#214430

Purpose

Dual-color reporter express eGFP constitutively; tdTomato expression is contingent on the inclusion of the frame-shifting cassette exon 7

Depositor

Chaolin Zhang

Article

Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

Insert

SMN2 EXON 7 (SMN2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-NLS-dPguCas13b-3xmRuby3-NLS

Plasmid

#132406

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPguCas13b

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

H151A; H1121A

Promoter

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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EF1a_SNAI2_P2A_Hygro_Barcode

Plasmid

#120478

Purpose

Barcoded lentiviral vector to express SNAI2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

SNAI2 (SNAI2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miRFP680-P2A-GFP

Plasmid

#197164

Purpose

Expresses the protein of miRFP680-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP680-P2A-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pLEX307 RFP

Plasmid

#163976

Purpose

Mammalian expression vector encoding RFP (for example, to normalize plasmid-based protein expression)

Depositor

Daniel Bachovchin

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

RFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-tTA

Plasmid

#99119

Purpose

An AAV genome that expresses tTA from the hSyn1 promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

tTA

Use

AAV

Tags

Expression

Mammalian

Mutation

*(see below)

Promoter

hSyn1

Available since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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LZF97_hSyn-spine-jRGECO1a

Plasmid

#119198

Purpose

Targets jRGECO1a to spines in living neurons.

Depositor

Don Arnold

Article

Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

Insert

jRGECO1a-TPR3-Utrophin(1-78)-TPR3-Zfn(CCR5-L) (Utrn Synthetic, Rat)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Human Synapsin 1

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pRSET YBX-1

Plasmid

#13035

Depositor

Peter Newburger

Article

Shen et al J Biol Chem. 1998 Mar 6. 273(10):5443-6.

Insert

YBX1 (YBX1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 5, 2006

Availability

Academic Institutions and Nonprofits only
epi-BE4max

Plasmid

#135975

Purpose

Expresses AncBE4max, EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin

Depositor

Yongming Wang

Article

Qi et al Front Cell Dev Biol. 2020 Sep 25;8:59058

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pLck-mVenus-C1

Plasmid

#84337

Purpose

Expresses a mVenus tagged with a membrane localization sequence

Depositor

Dorus Gadella

Article

van Unen et al BMC Cell Biol. 2018 Jun 7;19(1):6. doi:

Insert

Lck (MGCVCSSNPELPVAT)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 12, 2016

Availability

Academic Institutions and Nonprofits only

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Cytosolic Syk Biosensor

Plasmid

#125729

Purpose

FRET biosensor for studying SYK activities in cells

Depositor

Yingxiao Wang

Article

Xiang et al Cell Mol Bioeng. 2011 Dec;4(4):670-677.

Insert

SYK biosensor (ZAP70 Synthetic)

Use

Tags

6xHistine Tag at the N-terminal.

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-emiRFP670-P2A-GFP

Plasmid

#197160

Purpose

Expresses the protein of emiRFP670-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

emiRFP670-P2A-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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L4440_BioBrick-SCR-sgRNA-A

Plasmid

#177811

Purpose

Overexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)

Depositor

Michael Ristow

Article

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

Scrambled sgRNA targeting sequences

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-mRuby2

Plasmid

#99126

Purpose

An AAV genome that expresses the fluorescent protein mRuby2 from the hSyn1 promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mRuby2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn1

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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mCherry-Cytb5

Plasmid

#182579

Purpose

Expresses mCherry targeted to the ER via the CytB5RR tail anchor sequence

Depositor

Uri Manor

Article

Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

Insert

mCherry-Cytb5

Use

Tags

Cytb5

Expression

Mammalian

Mutation

Promoter

Available since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCW57-MCS1-P2A-MCS2-mDux

Plasmid

#138320

Purpose

Dox-inducible codon-optimized mouse Dux

Depositor

Yi Zhang

Article

Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

Insert

Dux (Dux Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pB-Neo-Mito-DsRed

Plasmid

#210328

Purpose

Overexpression of Mito-DsRed

Depositor

Li Yu

Article

Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

Insert

Mito-DsRed

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-mNeonGreen

Plasmid

#99131

Purpose

An AAV genome with tet-inducible expression of the fluorescent protein mNeonGreen

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mNeonGreen

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRIP3-mCherry

Plasmid

#61386

Purpose

Expresses RIP3-mCherry in mammalian cells

Depositor

Francis Chan

Article

Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

Insert

RIP3 (RIPK3 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only