We narrowed to 13,711 results for: TIM

1. pFH70

   Plasmid

   #128190

   Purpose

   Level1 ZmUBIp::nSpCas9-PmCDA (Arabidopsis codon optimized):NOSt

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   ZmUBIp::nSpCas9-PmCDA (Arabidopsis codon optimized):NOSt
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. IgH intron enhancer

   Plasmid

   #127247

   Purpose

   To maximize gene expression in B cells

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   heavy chain enhancer
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. KA2963_eSpCas9-PGKHygdtkCh

   Plasmid

   #124205

   Purpose

   Expression of eSpCas9(1.1) and sgRNA

   Depositor

   Hans Schöler

   Article

   Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. KA1063_pPB-hCMV*1-cHA-IRESH2BmKO2

   Plasmid

   #124169

   Purpose

   piggyBac-based Dox-inducible expression

   Depositor

   Hans Schöler

   Article

   Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pYZ292

   Plasmid

   #102686

   Purpose

   S. pombe gRNA entry vector with Cas9 - ScLEU2 marker

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. KA2895_pPB-TREtight-TATAmin-cHA-IRESH2BmKO2

   Plasmid

   #124174

   Purpose

   piggyBac-based Dox-inducible expression (weak)

   Depositor

   Hans Schöler

   Article

   Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. KA1123_pPB-TREtight-cHA-IRESH2BmKO2

   Plasmid

   #124172

   Purpose

   piggyBac-based Dox-inducible expression

   Depositor

   Hans Schöler

   Article

   Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. KFP14

   Plasmid

   #119796

   Purpose

   RpoD6 epitope tagged construct for integration at neutral site 1

   Depositor

   Erin O'Shea

   Article

   Fleming et al Cell Rep. 2018 Dec 11;25(11):2937-2945.e

   Insert

   Synpcc7942_1557 (Synpcc7942_1557 )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. KFP13

   Plasmid

   #119795

   Purpose

   RpoD5 epitope tagged construct for integration at neutral site 1

   Depositor

   Erin O'Shea

   Article

   Fleming et al Cell Rep. 2018 Dec 11;25(11):2937-2945.e

   Insert

   Synpcc7942_1849 (Synpcc7942_1849 )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. KFP06

    Plasmid

    #119799

    Purpose

    RpoD6 epitope tagged construct for integration at neutral site 2.2

    Depositor

    Erin O'Shea

    Article

    Fleming et al Cell Rep. 2018 Dec 11;25(11):2937-2945.e

    Insert

    Synpcc7942_1557 (Synpcc7942_1557 )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. KFP05

    Plasmid

    #119800

    Purpose

    RpoD5 epitope tagged construct for integration at neutral site 2.2

    Depositor

    Erin O'Shea

    Article

    Fleming et al Cell Rep. 2018 Dec 11;25(11):2937-2945.e

    Insert

    Synpcc7942_1849 (Synpcc7942_1849 )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHES945

    Plasmid

    #112643

    Purpose

    blue light inducible cryptochrome based optogenetic expression system

    Depositor

    Hana El-Samad

    Article

    Harrigan et al Cell. 2018 Oct 18;175(3):877-886.e10. do

    Insert

    CRY2PHR-GAL4DBD
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ReNL-Actin_pcDNA3

    Plasmid

    #89531

    Purpose

    Red color variant of bright luminescent protein, fused to actin

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    ReNL
    

    Use

    Tags

    Actin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. tubulin-OeNL_pcDNA3

    Plasmid

    #89523

    Purpose

    Orange color variant of bright luminescent protein, fused to b-tubulin

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    OeNL
    

    Use

    Tags

    b-tubulin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pVHed07

    Plasmid

    #82350

    Purpose

    Bxb1/TP901 temporal logic gate with mKate2 and sfGFP in Phi80 chromosomal integration plasmid

    Depositor

    Richard Murray

    Article

    Hsiao et al Mol Syst Biol. 2016 May 17;12(5):869. do

    Inserts

    mKate2 RFP
    Superfolder GFP
    Temporal logic gate with TP901 and Bxb1 attachment sites
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRSETb-pcDronpa

    Plasmid

    #78183

    Purpose

    Expresses pcDronpa in E. coli strains

    Depositor

    Peter Dedecker

    Article

    Moeyaert et al ACS Nano. 2014 Feb 25;8(2):1664-73. doi:

    Insert

    pcDronpa
    

    Use

    Tags

    6xHis-tag; Xpress tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pNB762

    Plasmid

    #60150

    Purpose

    Firefly luciferase (Promega) driven by LEU1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    FLuc has A4V & S504G (no functional effect)

    Promoter

    LEU1pr

    Available since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNB767

    Plasmid

    #60159

    Purpose

    Firefly luciferase (Promega) driven by SIC1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    A4V & S504G (no functional effect)

    Promoter

    SIC1pr

    Available since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNB649

    Plasmid

    #60136

    Purpose

    Firefly luciferase (Promega) driven by MET17 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    A4V

    Promoter

    MET17pr

    Available since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAW8ENdeI-cyEGFP-6XDSR

    Plasmid

    #49865

    Purpose

    for Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with 6 copies of the core DSR motif into the S. pombe urg1 locus

    Depositor

    Tony Carr

    Article

    Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    yEGFP

    Expression

    Mutation

    Promoter

    Available since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAW8ENdeI-cyEGFP-3XDSR

    Plasmid

    #49863

    Purpose

    for Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with 3 copies of the core DSR motif into the S. pombe urg1 locus

    Depositor

    Tony Carr

    Article

    Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    yEGFP

    Expression

    Mutation

    Promoter

    Available since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAW8ENdeI-c3HA-spo5DSR

    Plasmid

    #49872

    Purpose

    for Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locus

    Depositor

    Tony Carr

    Article

    Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    3XHA

    Expression

    Mutation

    Promoter

    Available since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAW8ENdeI-c3HA-6XDSR

    Plasmid

    #49870

    Purpose

    for Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with 6 copies of the core DSR motif into the S. pombe urg1 locus

    Depositor

    Tony Carr

    Article

    Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    3XHA

    Expression

    Mutation

    Promoter

    Available since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAW8ENdeI-c3HA-3XDSR

    Plasmid

    #49868

    Purpose

    for Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with 3 copies of the core DSR motif into the S. pombe urg1 locus

    Depositor

    Tony Carr

    Article

    Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    3XHA

    Expression

    Mutation

    Promoter

    Available since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAW8ENdeI-cyEGFP-8XDSR

    Plasmid

    #49866

    Purpose

    for Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with 8 copies of the core DSR motif into the S. pombe urg1 locus

    Depositor

    Tony Carr

    Article

    Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    yEGFP

    Expression

    Mutation

    Promoter

    Available since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAW8ENdeI-cyEGFP-4XDSR

    Plasmid

    #49864

    Purpose

    for Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with 4 copies of the core DSR motif into the S. pombe urg1 locus

    Depositor

    Tony Carr

    Article

    Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    yEGFP

    Expression

    Mutation

    Promoter

    Available since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAW8ENdeI-c3HA-8XDSR

    Plasmid

    #49871

    Purpose

    for Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with 8 copies of the core DSR motif into the S. pombe urg1 locus

    Depositor

    Tony Carr

    Article

    Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    3XHA

    Expression

    Mutation

    Promoter

    Available since

    Dec. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAW8ENdeI-c3HA-4XDSR

    Plasmid

    #49869

    Purpose

    for Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with 4 copies of the core DSR motif into the S. pombe urg1 locus

    Depositor

    Tony Carr

    Article

    Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    3XHA

    Expression

    Mutation

    Promoter

    Available since

    Dec. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFA6a-link-yomEmerald-CaURA3

    Plasmid

    #44890

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mEmerald with CaUra3 selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomEmerald
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFA6a-link-yoMaxGFP-CaURA3

    Plasmid

    #44875

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized MaxGFP with CaUra3 selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoMaxGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pFA6a-link-yoMaxGFP-SpHis5

    Plasmid

    #44840

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized MaxGFP with SpHis5 selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoMaxGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. -24lacZ

    Plasmid

    #25422

    Depositor

    David J. Goldhamer

    Article

    Chen et al Dev Dyn. 2001 Jul . 221(3):274-88.

    Insert

    Myo D 5' flanking sequence (MYOD1 Human)
    

    Use

    Reporter construct

    Tags

    lacZ

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 29, 2010

    Availability

    Academic Institutions and Nonprofits only

33. pRmHa3-dKDM4A-H195A-HA2FL2

    Plasmid

    #20235

    Depositor

    Jerry Workman

    Article

    Lin et al Mol Cell. 2008 Dec 5. 32(5):696-706.

    Insert

    dKDM4A (Kdm4A Fly)
    

    Use

    Tags

    FLAG2 and HA2

    Expression

    Insect

    Mutation

    changed His 195 to Ala

    Promoter

    Available since

    March 19, 2009

    Availability

    Academic Institutions and Nonprofits only

34. pRSET FLII282Pglu-4m

    Plasmid

    #13564

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

    Insert

    FLIPglu F16A T282S-ECFP-N283R
    

    Use

    Tags

    Citrine

    Expression

    Bacterial

    Mutation

    F16A, ECFP insert in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pICH47742::p35S-ePEmax3::tEURb7

    Plasmid

    #227692

    Purpose

    Golden Gate level 1, position 2, ePEmax3 expression cassette.

    Depositor

    Jae-Yean Kim

    Article

    Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

    Insert

    ePEmax3 being driven by long CaMV 35S (p35S) and terminated by a dual terminator (EURb7).
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    Nov. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pXPR_502

    Plasmid

    #96923

    Purpose

    for CRISPRa, lentiviral expression of gRNA scaffold using activator P65 HSF

    Depositor

    John Doench, David Root

    Article

    Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pICH47742:: pRPS5a-PE2max-NC::tHSP

    Plasmid

    #227694

    Purpose

    Golden Gate level 1, position 2, PE2max-NC expression cassette for Arabidopsis.

    Depositor

    Jae-Yean Kim

    Article

    Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

    Insert

    PE2max-NC being driven by pRPS5a and terminated by tHSP
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pRPS5a

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pSIRV-NFAT-eGFP

    Plasmid

    #118031

    Purpose

    Fluorescent reporter for NFAT activation

    Depositor

    Peter Steinberger

    Article

    Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

    Insert

    eGFP
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    minimal promoter with NFAT binding site

    Available since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Halo-Cyto

    Plasmid

    #124314

    Purpose

    Expresses cytosolic specific HaloTag protein

    Depositor

    Jin Wang

    Article

    Jiang et al Antioxid Redox Signal. 2018 Oct 25. doi:

    Insert

    Halo tag
    

    Use

    Tags

    Cytoplasm specific seq

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. ERroGFP_iE_pCDNA3

    Plasmid

    #47954

    Purpose

    Mammalian expression vector encoding for the ER targeted and FLAGM1 tagged redox probe roGFPiE (roGFP C147, E147b, C204)

    Depositor

    David Ron

    Article

    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

    Insert

    ERroGFP
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    insertion E147B

    Promoter

    Available since

    Oct. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge