We narrowed to 3,069 results for: FRI

1. pAAV-hSynapsin1-GCaMP6s-P2A-mKate2

   Plasmid

   #112006

   Purpose

   For co-expression of GCaMP6s with mKate2

   Depositor

   Lin Tian

   Article

   Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

   Has Service

   AAV9

   Insert

   GCaMP6s-P2A-mKate2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin1

   Available since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRC1765

   Plasmid

   #141346

   Purpose

   Mini plasmid containing minimal sequences for amplification in bacteria and two BbvCI nicking sites for introduction of modified DNA through annealing and ligation (e.g. mismatch, DNA lesions, flap).

   Depositor

   Rafael Fernandez-Leiro

   Article

   Fernandez-Leiro et al Nat Struct Mol Biol. 2021 Apr;28(4):373-

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. RS18960_pMQ30_KO_Kan

   Plasmid

   #185393

   Purpose

   Non-replicating vector used to create markerless deletion of RR42_RS18960 in C. basilensis 4G11

   Depositor

   Adam Arkin

   Article

   Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

   Inserts

   RR42_RS18960 upstream flanking homology region
   RR42_RS18960 downstream flanking homology region
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. CMVp-ECFP-Triplex-28-8xmiRNA-BS-28-pA (Construct 22)

   Plasmid

   #55199

   Purpose

   CFP reporter along with FF4 miRNA binding sites and 28 nt sequences

   Depositor

   Timothy Lu

   Article

   Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

   Insert

   ECFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. OVA-miSFIT-8-G

   Plasmid

   #124675

   Purpose

   Tuning expression of cytosolic Ovalbumin coupled to EGFP

   Depositor

   Tudor Fulga

   Article

   Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

   Insert

   Cyto-Ovalbumin
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Truncated OVA for cytoplasmic expression

   Promoter

   minimal CMV

   Available since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. PliaI

   Plasmid

   #55177

   Purpose

   Bacitracin-inducible promoter

   Depositor

   Thorsten Mascher

   Article

   Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

   Insert

   liaI promoter
   

   Use

   Synthetic Biology; Bacillus biobrick box

   Tags

   Expression

   Mutation

   Promoter

   liaI

   Available since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBR322-Pdp1_NTD-ZFN_L-HiBiT

   Plasmid

   #198319

   Purpose

   Loads HiBiT-tagged L fragment of a zinc finger nuclease specific for human AAVS1 locus using Pdp1 packaging domain

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   Pdp1_NTD-ZFN_L-HiBiT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-PIWI

   Plasmid

   #11591

   Depositor

   Edward Chan

   Article

   Jakymiw et al Nat Cell Biol. 2005 Dec . 7(12):1267-74.

   Insert

   Ago2 (AGO2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contain only aa482-869

   Promoter

   Available since

   June 30, 2006

   Availability

   Academic Institutions and Nonprofits only

9. RS17060_pMQ30_KO_Kan

   Plasmid

   #185396

   Purpose

   Non-replicating vector used to create markerless deletion of RR42_RS17060 in C. basilensis 4G11

   Depositor

   Adam Arkin

   Article

   Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

   Inserts

   RR42_RS17060 upstream flanking homology region
   RR42_RS17060 downstream flanking homology region
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. RS17055_pMQ30_KO_Kan

    Plasmid

    #185398

    Purpose

    Non-replicating vector used to create markerless deletion of RR42_RS17055 in C. basilensis 4G11

    Depositor

    Adam Arkin

    Article

    Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

    Inserts

    RR42_RS17055 upstream flanking homology region
    RR42_RS17055 downstream flanking homology region
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAP118

    Plasmid

    #105495

    Purpose

    pRPF185 derived plasmid encoding an anhydrotetracyclin inducible HupA-SmBiT and HupA-LgBiT fusions

    Depositor

    Wiep Klaas Smits

    Article

    Oliveira Paiva et al J Mol Biol. 2019 Jan 8. pii: S0022-2836(

    Insert

    HupA-SmBiT/HupA-LgBiT
    

    Use

    Atc-dependent expression in c. difficile

    Tags

    Expression

    Mutation

    Promoter

    Ptet

    Available since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. PliaG

    Plasmid

    #55174

    Purpose

    Constitutive promoter

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Insert

    LiaG promoter
    

    Use

    Synthetic Biology; Bacillus biobrick box

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. gCOTS-pyl

    Plasmid

    #92048

    Purpose

    This is a S. elongatus (PCC7942) shuttle vector used for recombination of the pylRS orthogonal translation system to genomic neutral site 2.

    Depositor

    Lital Alfonta

    Article

    Chemla et al Biochemistry. 2017 Apr 12. doi: 10.1021/

    Inserts

    Pyrrolysyl tRNA(cua)
    Pyrrolysyl tRNA synthetase (methanosarcina mazei)
    

    Use

    Cyanobacteria s. elongatus genome recombination i…

    Tags

    Expression

    Mutation

    Promoter

    LeuP (native S. elongatus promoter) and PrcbL

    Available since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAVsc CB6PI Gpluc7XmiR-122T

    Plasmid

    #35647

    Depositor

    Phillip Zamore

    Article

    Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

    Insert

    7 bulged miR-122 target sites
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CB

    Available since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. NHA-Ago1

    Plasmid

    #21535

    Depositor

    Edward Chan

    Article

    Lian et al RNA. 2009 May . 15(5):804-13.

    Insert

    Ago1 (AGO1 Human)
    

    Use

    Tags

    NHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 13, 2009

    Availability

    Academic Institutions and Nonprofits only

16. MC177VSG221RNAi

    Plasmid

    #59730

    Purpose

    For use in trypanosome cell lines already expressing T7 polymerase and Tet-repressor. Tetracycline induction results in the production of dsRNA targeting VSG221.

    Depositor

    Gloria Rudenko

    Article

    Sheader et al Proc Natl Acad Sci U S A. 2005 Jun 14;10

    Insert

    VSG221 RNAi fragment
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    opposing T7 promoters

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. J23101

    Plasmid

    #55176

    Purpose

    Very weak constitutive promoter

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Insert

    J23101 promoter
    

    Use

    Synthetic Biology; Bacillus biobrick box

    Tags

    Expression

    Mutation

    Promoter

    J23101

    Available since

    July 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV TBG FFluc miR122sponge

    Plasmid

    #35657

    Depositor

    Phillip Zamore

    Article

    Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

    Insert

    miR-122 sponge
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    TBG

    Available since

    July 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28-sfGFP-SpyTag003 Loop A

    Plasmid

    #171776

    Purpose

    Expresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Val22 and Asn23

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    sfGFP-SpyTag003 Loop A
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    SpyTag003 and linkers inserted between residues V…

    Promoter

    T7

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. Cdc42 T17N sp64T

    Plasmid

    #16672

    Depositor

    De-Li Shi

    Article

    Djiane et al Development. 2000 Jul . 127(14):3091-100

    Insert

    Cdc42 T17N (CDC42 Human)
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    T17N. Dominant negative.

    Promoter

    Available since

    April 28, 2008

    Availability

    Academic Institutions and Nonprofits only