We narrowed to 2,682 results for: Kl;

1. pLKO-puro human lipin 1-1 shRNA

   Plasmid

   #32019

   Depositor

   David Sabatini

   Article

   Peterson et al Cell. 2011 Aug 5;146(3):408-20.

   Insert

   lipin1 shRNA
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

2. SpyCas9-AIDx

   Plasmid

   #135343

   Purpose

   Expression of wildtype nSpyCas9 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSpyCas9-hAIDx
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCSDest2-NmeCas9-NLS-3XHA-NLS

   Plasmid

   #87448

   Purpose

   Mammalian expression of wild type Nme Cas9

   Depositor

   Erik Sontheimer

   Article

   Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

   Insert

   Nme Cas9
   

   Use

   CRISPR

   Tags

   3XHA, SV40 NLS, and cMyc-like NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCherry-H2B-6

   Plasmid

   #55056

   Purpose

   Localization: Nucleus/Histones, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Article

   Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

   Insert

   H2B (H2BC11 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   D26G and V119I in H2B

   Promoter

   CMV

   Available since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pCRISPR_DMS3g24

   Plasmid

   #89244

   Purpose

   Plasmid encodes a synthetic CRISPR containing 7 copies of a single spacer, which targets a region (gene 24) in the genome of bacteriophage DMS3

   Depositor

   Blake Wiedenheft

   Article

   Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:

   Insert

   CRISPR
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-mCherry-TSC2(AA)

   Plasmid

   #192442

   Purpose

   Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation.

   Depositor

   Jin Zhang

   Article

   Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

   Insert

   mCherry-TSC2(S939A,T1439A) (TSC2 Human)
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

   Promoter

   CMV

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. ABEMax nSaCas9

   Plasmid

   #135364

   Purpose

   Expresion of SaCas9 nickase (D10A) with ABEMax at the N terminus

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   ABEMax nSaCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   (V82G)

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3 IKKe T7

   Plasmid

   #27238

   Depositor

   Tom Maniatis

   Article

   Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

   Insert

   I-kappa-B kinase epsilon (IKBKE Human)
   

   Use

   Tags

   T7

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

9. mOrange2-Clathrin-15

   Plasmid

   #57947

   Purpose

   Localization: Clathrin Vesicles, Excitation: 549, Emission: 565

   Depositor

   Michael Davidson

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Insert

   Clathrin (CLTB Human)
   

   Use

   Tags

   mOrange2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pMRX-IPU-FLAG-NEK9T210A

    Plasmid

    #168270

    Purpose

    Expresses kinase-dead NEK9 mutant in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi:

    Insert

    NEK9 (NEK9 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed threonine 210 to alanine

    Promoter

    Available since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRL K-ras 3'UTR mutant

    Plasmid

    #14805

    Depositor

    Tyler Jacks

    Article

    Kumar et al Nat Genet. 2007 Apr 1. ():.

    Insert

    K-ras 3'UTR (Kras Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    let-7 miRNA binding site mutations

    Promoter

    Available since

    April 6, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pKM294

    Plasmid

    #134179

    Purpose

    Mammalian expression of CENP-C and KNL2

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

    Insert

    mcherry-CENP-C 510-end-KNL2 (CENPC Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEJS482-pCDest2-AcrIIC3Nme-mTagBFP2-IRES

    Plasmid

    #85750

    Purpose

    Expresses Type II-C anti-CRISPR protein, AcrIIC3Nme from Neisseria meningitidis, and mTagBFP2

    Depositor

    Erik Sontheimer

    Article

    Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

    Inserts

    Type II-C anti-CRISPR AcrIIC3Nme
    mTagBFP2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUASP dsRed Rab11 WT

    Plasmid

    #44829

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab11 (Rab11 Fly)
    

    Use

    Drosophila

    Tags

    dsRed

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 18, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pCRII-Topo Cav1 in situ probe

    Plasmid

    #45624

    Depositor

    Jeffrey Macklis

    Article

    Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

    Insert

    Cav1 in situ probe (Cav1 Mouse)
    

    Use

    In situ

    Tags

    Expression

    Mutation

    fragment contains bp# 1472-2124 of AB029929.1

    Promoter

    Available since

    July 9, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pEJS481-pCDest2-AcrE2-mTagBFP2-IRES

    Plasmid

    #85749

    Purpose

    Expresses anti-CRISPR protein from Type I-E and mTagBFP2

    Depositor

    Erik Sontheimer

    Article

    Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

    Inserts

    Type I-E anti-CRISPR
    mTagBFP2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBEG R2-iCreT2ALuc-L3 #BGV208

    Plasmid

    #48992

    Purpose

    Supplies Cre recombinase and Luciferase and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

    Depositor

    David Dankort

    Article

    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

    Inserts

    cre recombinase
    Luciferase
    

    Use

    Luciferase; Gateway cloning vector

    Tags

    IRES (weak)

    Expression

    Mutation

    Promoter

    Unknown

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pBEG R2-iPuro-L3 #BGV205

    Plasmid

    #48987

    Purpose

    Supplies the selection marker (puromycin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

    Depositor

    David Dankort

    Article

    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

    Insert

    puromycin resistance
    

    Use

    Gateway cloning vector

    Tags

    IRES (weak)

    Expression

    Mutation

    Promoter

    NA

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pENTR-OsMIR390-B/c

    Plasmid

    #61468

    Purpose

    Gateway-compatible entry vector for direct cloning of artificial microRNAs into rice MIR390 precursor.

    Depositor

    James Carrington

    Article

    Carbonell et al Plant J. 2015 Mar 24. doi: 10.1111/tpj.1

    Insert

    OsMIR390-B/c
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    O. sativa MIR390 precursor sequence including a c…

    Promoter

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX 4T1 ERbeta(144-530)

    Plasmid

    #35566

    Depositor

    Harish Srinivas

    Article

    Zhang et al Mol Endocrinol. 2010 Sep;24(9):1737-47.

    Insert

    Estrogen Receptor beta (ESR2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    residues 144-530

    Promoter

    Available since

    March 26, 2012

    Availability

    Academic Institutions and Nonprofits only