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Plasmid#183554PurposeDesigned to test the efficacy of shRNA by assessing the ratio of GFP-fusion protein to RFP (shRNA ratio negative control (Luciferase), Fig S2)DepositorInsertLuciferase shRNA
UseTagsExpressionMammalianMutationPromoterCBhAvailable sinceJune 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
Rp51a_10bp 5'SS_No BP or pBP_pUC18
Plasmid#100989PurposeRp51a with a tGGTAtGTta->AGGTAAGTAT 5'SS mutant with potential to form 10bps with the U1 snRNA. Also contains TACTAAC->GTTAGTG BP mutataion and a TACAAAC->GTTTGTG pseudo BP mutationDepositorInsertRP51A (RPS17A Budding Yeast)
UseTagsExpressionBacterial and YeastMutationtGGTAtGTta->AGGTAAGTAT 5'SS mutant, TACTA…PromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Rp51a_SUS1 5'ss_No_BP_pBP-pUC18
Plasmid#100990PurposeContains model Rp51a with a GGTAtGT->tGTAtGa 5'SS mutant (SUS1 5'SS sequence). Also contains TACTAAC->GTTAGTG BP mutataion and a TACAAAC->GTTTGTG pseudo BP mutationDepositorInsertRP51A (RPS17A Budding Yeast)
UseTagsExpressionBacterial and YeastMutationGGTAtGT->tGTAtGa 5'SS mutant, TACTAAC->…PromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
TLCV2-sgHPDL #9
Plasmid#174166Purposeknock out HPDL in human cell linesDepositorInsertsgRNA against HPDL
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTX1-SMN2-exon7-ESE1
Plasmid#126042PurposeIn-vitro-transcription of SMN2-exon7-ESE1 RNA in NMR tube for "Systems NMR" analysisDepositorInsertSMN2 exon7 ESE1
UseTagsExpressionMutationPromoterAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
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MLSE-shTAF12.376
Plasmid#105578Purposeretrovirally express TAF12 shRNA with GFP markerDepositorInsertTAF12 shRNA #376
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterMSCV-LTRAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
LEP-shTAF4.2818
Plasmid#105563Purposeretrovirally express TAF4 shRNA with puro resistance and GFP markerDepositorInsertTAF4 shRNA
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterMSCV-LTRAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
LEP-shTAF2.4392
Plasmid#105562Purposeretrovirally express TAF2 shRNA with puro resistance and GFP markerDepositorInsertTAF2 shRNA
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterMSCV-LTRAvailable sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
LEP-shTAF12.376
Plasmid#105560Purposeretrovirally express TAF12 shRNA with puro resistance and GFP markerDepositorInsertTAF12 shRNA
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterMSCV-LTRAvailable sinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
PX458_ZNF511_1
Plasmid#104044PurposeEncodes gRNA for 3' target of human ZNF511 along with Cas9 with 2A GFPDepositorInsertZNF511 gRNA (ZNF511 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_ZNF511_2
Plasmid#104045PurposeEncodes gRNA for 3' target of human ZNF511 along with Cas9 with 2A GFPDepositorInsertZNF511 gRNA (ZNF511 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCm7RNA6A
Plasmid#84367PurposeCas9-gRNA plasmid for mouse Cbfa2t2 m7 mutant knockinDepositorInsertCbfa2t2 m7-gRNA6
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_ZNF644_2
Plasmid#86306PurposeEncodes gRNA for 3' target of human ZNF644DepositorInsertgRNA against ZNF644 (ZNF644 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
MXW-PGK-c-let-7-LP4B-IRES-EGFP
Plasmid#37647DepositorInsertcel-let-7
UseRetroviralTagsExpressionMammalianMutationLP4 - AATATTACCA=>AATAAAACCAPromotermPGKAvailable sinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
MXW-PGK-c-let-7-LP4A-IRES-EGFP
Plasmid#37646DepositorInsertcel-let-7
UseRetroviralTagsExpressionMammalianMutationLP4 - AATATTACCA=>AATAAAACCAPromotermPGKAvailable sinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
MXW-PGK-c-let-7-LP4-IRES-EGFP
Plasmid#37643DepositorInsertcel-let-7
UseRetroviralTagsExpressionMammalianMutationLP4 - AATATTACCA=>AATAAAACCAPromotermPGKAvailable sinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR v2 hBAX
Plasmid#129580PurposeCRISPR deletion of human BAXDepositorInsertgRNA vs human BAX (BAX Human)
UseLentiviralTagsExpressionMammalianMutationNonePromoterAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 puro_shDDX58_230212
Plasmid#167293PurposeLentiviral plKO.1 construct coding for a short-hairpin RNA targeting the human gene DDX58 (coding for RLR sensor RIG-I). Contains a puro resistance cassette.DepositorInsertshRNA hsDDX58 (RIGI Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only