We narrowed to 3,053 results for: eed

1. pGL3-321/+120 Ebox mut2

   Plasmid

   #35545

   Depositor

   Greg Goodall

   Article

   Bracken et al Cancer Res. 2008 Oct 1;68(19):7846-54.

   Insert

   promoter region of miR200b-200a-429 genomic cluster (MIR200B, MIR429, MIR200A Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mutation

   T to G at position -52 in promoter

   Promoter

   none

   Available since

   June 25, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. psiCHECK2-AURKB 3'UTR

   Plasmid

   #29475

   Depositor

   Judy Lieberman

   Article

   Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

   Insert

   AURKB 3"UTR (AURKB Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 27, 2011

   Availability

   Academic Institutions and Nonprofits only

3. CMV-FFluc-GPD1L-3'UTR Full mut

   Plasmid

   #32489

   Depositor

   Pere Puigserver

   Article

   Kelly et al Mol Cell Biol. 2011 Jul;31(13):2696-706.

   Insert

   GPD1L 3'UTR (Gpd1l Mouse)
   

   Use

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Mutated in the entire miR-210 binding region

   Promoter

   CMV

   Available since

   Nov. 4, 2011

   Availability

   Academic Institutions and Nonprofits only

4. H2B-GFP-Aurora B KD

   Plasmid

   #184043

   Purpose

   Expresses H2B-GFP fused to Aurora B kinase dead

   Depositor

   Lienhard Schmitz

   Article

   Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

   Insert

   AURKB (AURKB Human)
   

   Use

   Tags

   EGFP and H2B

   Expression

   Mammalian

   Mutation

   Changed Lysine 65 to Arginine

   Promoter

   CMV

   Available since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-Repo-Man-1-890

   Plasmid

   #184272

   Purpose

   Expresses C-terminally truncated GFP-RepoMan

   Depositor

   Lienhard Schmitz

   Article

   Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

   Insert

   cell division cycle associated 2 (CDCA2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   truncated Repo-Man after aminoacid 893

   Promoter

   CMV

   Available since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-RepoMan-S893D

   Plasmid

   #204995

   Purpose

   Expresses shRNA resistant GFP-RepoMan S893D Mutant

   Depositor

   Lienhard Schmitz

   Article

   Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

   Insert

   cell division cycle associated 2 (CDCA2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   changed Serine 893 to Aspartic acid

   Promoter

   CMV

   Available since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. psiCHECK-VIM270-pCM

   Plasmid

   #215869

   Purpose

   This plasmid encodes luciferaase which has siRNA binding sites in 3'UTR for detecting the knockdown efficiency of siRNA.

   Depositor

   Kumiko Ui-Tei

   Article

   Iribe et al ACS Omega. 2017 May 31;2(5):2055-2064. d

   Insert

   target site which is completely complementary to passanger strand of siRNA for vimentin is inserted to 3'UTR of RLuc (VIM Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. FLAG-mFzd4-opt(40-537)-9xHis_pFastBac1

   Plasmid

   #216378

   Purpose

   Baculovirus transfer vector to express FLAG-tagged mouse Fzd4 (codon-optimized for insect cell expression)

   Depositor

   William Weis

   Article

   Mahoney et al Sci Signal. 2022 Aug 23;15(748):eabo2820

   Insert

   Frizzled-4 (Fzd4 Mouse)
   

   Use

   Baculovirus

   Tags

   HA signal sequence-FLAG

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV Flag-LRRK2 K17A/K18A/R1441G

   Plasmid

   #186013

   Purpose

   Flag-LRRK2 K17A/K18A/R1441G

   Depositor

   Suzanne Pfeffer

   Article

   Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

   Insert

   Flag-LRRK2 K17A/K18A/R1441G (LRRK2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   See depositor's comments below.

   Promoter

   Available since

   Sept. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a Cdk2ap1ΔN (MT2B2)-MutTER

    Plasmid

    #178035

    Purpose

    Expression vector for the purpose of protein purification.

    Depositor

    Lin He

    Article

    Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

    Insert

    Cdk2ap1 (Cdk2ap1 Mouse)
    

    Use

    Nonviral

    Tags

    6xHIS - Thrombin - MBP- TEV-TRS

    Expression

    Bacterial

    Mutation

    First N-Terminal 27aa are deleted and Changed T(8…

    Promoter

    T7LacI

    Available since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a Cdk2ap1CAN-MutTER

    Plasmid

    #178033

    Purpose

    Expression vector for the purpose of protein purification.

    Depositor

    Lin He

    Article

    Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

    Insert

    Cdk2ap1 (Cdk2ap1 Mouse)
    

    Use

    Nonviral

    Tags

    6xHIS - Thrombin - MBP- TEV-TRS

    Expression

    Bacterial

    Mutation

    Changed T(108)A, E(109)A, R(110)A

    Promoter

    T7LacI

    Available since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a Cdk2ap1ΔN (MT2B2)

    Plasmid

    #178034

    Purpose

    Expression vector for the purpose of protein purification.

    Depositor

    Lin He

    Article

    Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

    Insert

    Cdk2ap1 (Cdk2ap1 Mouse)
    

    Use

    Nonviral

    Tags

    6xHIS - Thrombin - MBP- TEV-TRS

    Expression

    Bacterial

    Mutation

    First N-Terminal 27aa are deleted

    Promoter

    T7LacI

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. psiCheck Cdk2ap1ΔN (MT2B2)

    Plasmid

    #178029

    Purpose

    expression vector to test translation efficiency by luciferase reporter.

    Depositor

    Lin He

    Article

    Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

    Insert

    Cdk2ap1 (Cdk2ap1 Mouse)
    

    Use

    Luciferase; Nonviral

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Cloned in

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Psicheck Cdk2ap1Exon1

    Plasmid

    #178028

    Purpose

    expression vector to test translation efficiency by luciferase reporter.

    Depositor

    Lin He

    Article

    Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

    Insert

    Cdk2ap1 (Cdk2ap1 Mouse)
    

    Use

    Luciferase; Nonviral

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Cloned in

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEN_TT 3xFLAG-MAVS-Ala271

    Plasmid

    #158631

    Purpose

    Gateway entry vector for an inducible 3xFLAG-tagged MAVS mutant

    Depositor

    Kevin Janes

    Article

    Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

    Insert

    MAVS (MAVS Human)
    

    Use

    Gateway entry vector

    Tags

    3XFLAG

    Expression

    Mutation

    Q271A please see depositor comments below

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSLIK 3xFLAG-MAVS-Ala271 hygro

    Plasmid

    #158637

    Purpose

    Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant construct

    Depositor

    Kevin Janes

    Article

    Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

    Insert

    MAVS (MAVS Human)
    

    Use

    Lentiviral

    Tags

    3XFLAG

    Expression

    Mutation

    Q271A

    Promoter

    Available since

    Nov. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSLIK 3xFLAG-MAVS-Gln93,Ala271 hygro

    Plasmid

    #158638

    Purpose

    Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant construct

    Depositor

    Kevin Janes

    Article

    Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

    Insert

    MAVS (MAVS Human)
    

    Use

    Lentiviral

    Tags

    3XFLAG

    Expression

    Mutation

    E93Q/Q271A

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEN_TT 3xFLAG-MAVS-Gln93,Ala271

    Plasmid

    #158632

    Purpose

    Gateway entry vector for an inducible 3xFLAG-tagged MAVS mutant

    Depositor

    Kevin Janes

    Article

    Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

    Insert

    MAVS (MAVS Human)
    

    Use

    Gateway entry vector

    Tags

    3XFLAG

    Expression

    Mutation

    E93Q/Q271A

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEN_TT 3xFLAG-MAVS-Ala148,Ala271

    Plasmid

    #158633

    Purpose

    Gateway entry vector for an inducible 3xFLAG-tagged MAVS mutant

    Depositor

    Kevin Janes

    Article

    Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

    Insert

    MAVS (MAVS Human)
    

    Use

    Gateway entry vector

    Tags

    3XFLAG

    Expression

    Mutation

    Q148A/Q271A

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSLIK 3xFLAG-MAVS-Ala148,Ala271 hygro

    Plasmid

    #158639

    Purpose

    Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant construct

    Depositor

    Kevin Janes

    Article

    Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

    Insert

    MAVS (MAVS Human)
    

    Use

    Lentiviral

    Tags

    3XFLAG

    Expression

    Mutation

    Q148A/Q271A

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge