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Plasmid#204995PurposeExpresses shRNA resistant GFP-RepoMan S893D MutantDepositorInsertcell division cycle associated 2 (CDCA2 Human)
UseTagsGFPExpressionMammalianMutationchanged Serine 893 to Aspartic acidPromoterCMVAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-VIM270-pCM
Plasmid#215869PurposeThis plasmid encodes luciferaase which has siRNA binding sites in 3'UTR for detecting the knockdown efficiency of siRNA.DepositorInserttarget site which is completely complementary to passanger strand of siRNA for vimentin is inserted to 3'UTR of RLuc (VIM Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
FLAG-mFzd4-opt(40-537)-9xHis_pFastBac1
Plasmid#216378PurposeBaculovirus transfer vector to express FLAG-tagged mouse Fzd4 (codon-optimized for insect cell expression)DepositorInsertFrizzled-4 (Fzd4 Mouse)
UseBaculovirusTagsHA signal sequence-FLAGExpressionInsectMutationPromoterPolyhedrinAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV Flag-LRRK2 K17A/K18A/R1441G
Plasmid#186013PurposeFlag-LRRK2 K17A/K18A/R1441GDepositorInsertFlag-LRRK2 K17A/K18A/R1441G (LRRK2 Human)
UseTagsExpressionMammalianMutationSee depositor's comments below.PromoterAvailable sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a Cdk2ap1ΔN (MT2B2)-MutTER
Plasmid#178035PurposeExpression vector for the purpose of protein purification.DepositorInsertCdk2ap1 (Cdk2ap1 Mouse)
UseNonviralTags6xHIS - Thrombin - MBP- TEV-TRSExpressionBacterialMutationFirst N-Terminal 27aa are deleted and Changed T(8…PromoterT7LacIAvailable sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a Cdk2ap1CAN-MutTER
Plasmid#178033PurposeExpression vector for the purpose of protein purification.DepositorInsertCdk2ap1 (Cdk2ap1 Mouse)
UseNonviralTags6xHIS - Thrombin - MBP- TEV-TRSExpressionBacterialMutationChanged T(108)A, E(109)A, R(110)APromoterT7LacIAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a Cdk2ap1ΔN (MT2B2)
Plasmid#178034PurposeExpression vector for the purpose of protein purification.DepositorInsertCdk2ap1 (Cdk2ap1 Mouse)
UseNonviralTags6xHIS - Thrombin - MBP- TEV-TRSExpressionBacterialMutationFirst N-Terminal 27aa are deletedPromoterT7LacIAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
psiCheck Cdk2ap1ΔN (MT2B2)
Plasmid#178029Purposeexpression vector to test translation efficiency by luciferase reporter.DepositorInsertCdk2ap1 (Cdk2ap1 Mouse)
UseLuciferase; NonviralTagsLuciferaseExpressionMammalianMutationPromoterCloned inAvailable sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
Psicheck Cdk2ap1Exon1
Plasmid#178028Purposeexpression vector to test translation efficiency by luciferase reporter.DepositorInsertCdk2ap1 (Cdk2ap1 Mouse)
UseLuciferase; NonviralTagsLuciferaseExpressionMammalianMutationPromoterCloned inAvailable sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG-MAVS-Ala271
Plasmid#158631PurposeGateway entry vector for an inducible 3xFLAG-tagged MAVS mutantDepositorInsertMAVS (MAVS Human)
UseGateway entry vectorTags3XFLAGExpressionMutationQ271A please see depositor comments belowPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3xFLAG-MAVS-Ala271 hygro
Plasmid#158637PurposeLentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant constructDepositorInsertMAVS (MAVS Human)
UseLentiviralTags3XFLAGExpressionMutationQ271APromoterAvailable sinceNov. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3xFLAG-MAVS-Gln93,Ala271 hygro
Plasmid#158638PurposeLentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant constructDepositorInsertMAVS (MAVS Human)
UseLentiviralTags3XFLAGExpressionMutationE93Q/Q271APromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG-MAVS-Gln93,Ala271
Plasmid#158632PurposeGateway entry vector for an inducible 3xFLAG-tagged MAVS mutantDepositorInsertMAVS (MAVS Human)
UseGateway entry vectorTags3XFLAGExpressionMutationE93Q/Q271APromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG-MAVS-Ala148,Ala271
Plasmid#158633PurposeGateway entry vector for an inducible 3xFLAG-tagged MAVS mutantDepositorInsertMAVS (MAVS Human)
UseGateway entry vectorTags3XFLAGExpressionMutationQ148A/Q271APromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3xFLAG-MAVS-Ala148,Ala271 hygro
Plasmid#158639PurposeLentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant constructDepositorInsertMAVS (MAVS Human)
UseLentiviralTags3XFLAGExpressionMutationQ148A/Q271APromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3xFLAG-MAVS-Ala148 hygro
Plasmid#158636PurposeLentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant constructDepositorInsertMAVS (MAVS Human)
UseLentiviralTags3XFLAGExpressionMutationQ148APromoterAvailable sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3xFLAG-MAVS-Gln93 hygro
Plasmid#158635PurposeLentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant constructDepositorInsertMAVS (MAVS Human)
UseLentiviralTags3XFLAGExpressionMutationE93QPromoterAvailable sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR_P4-P1R:PER36modp
Plasmid#128431PurposeGateway (Invitrogen) promoter clone (pDONR_P4-P1R) with premature stop codons introduced within the Xero1 and Xero2 genes in the PER36 promoter entry clone (Kunieda et al. 2013).DepositorInsertPEROXISADE36 (AT3G50990 Mustard Weed)
UseGateway promoter entry cloneTagsExpressionMutationPremature stop codons introduced into the Xero1 (…PromoterPEROXISADE36Available sinceOct. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSB61 - pL0_V2 (CDS1)
Plasmid#123183PurposeGolden Gate (MoClo; CDS1) compatible Tomato yellow leaf curl virus (TYLCV) V2 silencing suppressor based on NCBI Reference Sequence NC_004005DepositorInsertTomato yellow leaf curl virus (TYLCV) V2 (v2 Synthetic)
UsePart for plant expressionTagsExpressionPlantMutationNo BpiI and BsaI sitesPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pABD823
Plasmid#67911PurposeProtein interaction with yeast two hybrid assay of CTG10 in yeast, Saccharomyces cerevisiaeDepositorInsertCold temperature germinating 10 (CTG10)
UseTagsGAL4 DNA binding domain (148 amino acids)ExpressionYeastMutationPromoterpADH1 (alcohol dehydrogenase 1 promoter)Available sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only