59,428 results

1. AICSDP-41: ATP2A2-mEGFP

   Plasmid

   #114405

   Purpose

   Homology arms and mEGFP-linker sequence for N-terminus tagging of human ATP2A2

   Depositor

   Allen Institute for Cell Science

   Insert

   ATP2A2 Homology Arms with mEGFP-linker (ATP2A2 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   Sept. 4, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pEIGW Grx1-roGFP2

   Plasmid

   #64990

   Purpose

   Mammalian expression of cytosolic Grx1-roGFP2 (lentiviral vector)

   Depositor

   Tobias Dick

   Article

   Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

   Insert

   Glutaredoxin-1 (GLRX Human)
   

   Use

   Lentiviral

   Tags

   roGFP2

   Expression

   Mammalian

   Available Since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. R989-X08-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G12C

   Plasmid

   #159540

   Purpose

   E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G12C

   Depositor

   Dominic Esposito

   Article

   Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

   Insert

   His6-MBP-tev-G-Hs.KRAS4b(1-169) G12C (KRAS Human)
   

   Tags

   His6-MBP-tev

   Expression

   Bacterial

   Mutation

   G12C

   Available Since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDH-puro-EWS-FLI1

   Plasmid

   #102813

   Purpose

   Overexpress EWS-FLI1. 3rd generation lentiviral vector.

   Depositor

   Jialiang Wang

   Article

   Loganathan et al Oncotarget. 2016 Jul 12;7(28):43504-4351

   Insert

   EWS-FLI1 (EWSR1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. VPS13C^Halo-△(ATG2C-PH)

   Plasmid

   #232867

   Purpose

   Expresses VPS13C-△(ATG2C-PH) in mammalian cells

   Depositor

   Pietro De Camilli

   Article

   Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

   Insert

   VPS13C-△(ATG2C-PH) (VPS13C Human)
   

   Tags

   internal Halo

   Expression

   Mammalian

   Available Since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCherry-PH(VPS13C)

   Plasmid

   #232869

   Purpose

   Expresses the PH domain of VPS13C in mammalian cells

   Depositor

   Pietro De Camilli

   Article

   Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

   Insert

   the PH domain of VPS13C (VPS13C Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCherry-VAB(VPS13C)

   Plasmid

   #232865

   Purpose

   Expresses the VAB domain of VPS13C in mammalian cells

   Depositor

   Pietro De Camilli

   Article

   Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

   Insert

   the VAB domain of VPS13C (VPS13C Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR223_PAK1_WT

   Plasmid

   #82220

   Purpose

   Gateway Donor vector containing PAK1 , part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, William Hahn, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   PAK1 (PAK1 Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV4-ApoE2

   Plasmid

   #87085

   Purpose

   Expresses ApoE2 in Mammalian cells

   Depositor

   Bradley Hyman

   Article

   Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

   Insert

   Apolipoprotein E2 (APOE Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. PH-PLCd1-mNGx1

    Plasmid

    #202751

    Purpose

    expresses fluorescent protein-tagged lipid biosensor

    Depositor

    Gerry Hammond

    Article

    Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

    Insert

    PLCD1v2(1-170):mNG2 (PLCD1 Human)
    

    Expression

    Mammalian

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLQ-mgU2-47-Mango II

    Plasmid

    #127586

    Purpose

    Expresses mgU2-47-Mango II scaRNA from a mU6 promoter

    Depositor

    David Rueda

    Article

    Autour et al Nat Commun. 2018 Feb 13;9(1):656. doi: 1

    Insert

    mgU2-47 scaRNA with Mango II tag
    

    Expression

    Mammalian

    Promoter

    modified U6

    Available Since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-KIT-V5/HIS

    Plasmid

    #201986

    Purpose

    expression of human KIT receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    KIT receptor tyrosine kinase (KIT Human)
    

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. 3xFlag-tdMCP-Erk2 (pErk reporter)

    Plasmid

    #226167

    Purpose

    Used to transiently express the pErk reporter in LABEL-seq MAPK activity assays.

    Depositor

    Dustin Maly

    Article

    Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

    Insert

    3xFlag-tdMCP-Erk2 (MAPK1 Human)
    

    Tags

    3xFlag-tdMCP

    Expression

    Mammalian

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. HIS-HA-CMYC P53

    Plasmid

    #233738

    Purpose

    expresses HIS-HA-CMYC triple tagged P53 for pulldown assay

    Depositor

    Albert Keung

    Article

    Han et al Methods. 2025 Mar;235:92-99. doi: 10.101

    Insert

    HIS-HA-CMYC-p53 (TP53 Human)
    

    Tags

    HIS-HA-CMYC

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. RMCE ABCDE>ala DNA-PKcs

    Plasmid

    #233914

    Purpose

    Low copy number provides expression of human DNA-PKcs with the 6 ABCDE phosphorylation sites substituted to alanine.

    Depositor

    Katheryn Meek

    Article

    Buehl et al Mol Cell. 2023 Mar 2;83(5):698-714.e4. d

    Insert

    human DNA-PKcs ABCDE phosphorylation site mutant (PRKDC Human)
    

    Expression

    Mammalian

    Mutation

    phosphorylation sites 2609, 2612, 2620, 2624, 263…

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-CRX-T2A-NeuroD1

    Plasmid

    #194607

    Purpose

    Piggybac Tet-ON plasmid for inducing hiPSCs into photoreceptor-like cells via CRX and NeuroD1 expression.

    Depositor

    Haruhisa Inoue

    Article

    Otsuka et al iScience. 2022 Mar 8;25(4):103987. doi:

    Insert

    CRX-T2A-NeuroD1
    

    Use

    Piggybac transposon

    Expression

    Mammalian

    Promoter

    tetO

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET15b-His-3C-irisin

    Plasmid

    #122612

    Purpose

    Expresses in E. coli: human irisin with Nt histag and 3C protease site for cleavage

    Depositor

    Harold Erickson

    Article

    Albrecht et al Sci Rep. 2015 Mar 9;5:8889. doi: 10.1038

    Insert

    irisin (ectodomain of FNDC5) (Fndc5 Human, Mouse, Rat, Bovine)
    

    Tags

    Nt: histag-3C protease site

    Expression

    Bacterial

    Promoter

    T7 polymerase

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pD649-HAsp-KIR2DL5A-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156643

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    KIR2DL5A (KIR2DL5A Human)
    

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHLSec2-irisin-his

    Plasmid

    #122729

    Purpose

    Expresses in mammalian cells (HEK293):glycosylated and secreted irisin-his

    Depositor

    Harold Erickson

    Article

    Albrecht et al Sci Rep. 2015 Mar 9;5:8889. doi: 10.1038

    Insert

    irisin (ectodomain of FNDC5) (Fndc5 Human, Mouse, Rat, Bovine)
    

    Tags

    his and signal sequence for secretion

    Expression

    Mammalian

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. TFORF1932

    Plasmid

    #142292

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    JUN (JUN Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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