We narrowed to 6,165 results for: cas9 expression plasmid

1. pHOXB13.1.0-gDNA

   Plasmid

   #113782

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor HOXB13

   Depositor

   Kevin White

   Insert

   HOXB13 (HOXB13 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGLIS3.1.0-gDNA

   Plasmid

   #113780

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor GLIS3

   Depositor

   Kevin White

   Insert

   GLIS3 (GLIS3 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSRF.1.0-gDNA

   Plasmid

   #112458

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor SRF

   Depositor

   Kevin White

   Insert

   SRF (SRF Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEAF1.2.1-gDNA

   Plasmid

   #112449

   Purpose

   CRISPR plasmid for expression of Cas9 nickase (D10A) and gRNA targeting human transcription factor DEAF1

   Depositor

   Kevin White

   Insert

   DEAF1 (DEAF1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pE2F5.1.0-gDNA

   Plasmid

   #112447

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor E2F5

   Depositor

   Kevin White

   Insert

   E2F5 (E2F5 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDLX1.1.0-gDNA

   Plasmid

   #112439

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor DLX1

   Depositor

   Kevin White

   Insert

   DLX1 (DLX1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHBP1.1.0-gDNA

   Plasmid

   #112432

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor HBP1

   Depositor

   Kevin White

   Insert

   HBP1 (HBP1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pATF1.1.0-gDNA

   Plasmid

   #112424

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ATF1

   Depositor

   Kevin White

   Insert

   ATF1 (ATF1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSL1180-HR-Aag2-loxP-PUb-RFP-loxP-3xFLAG-AGO1

   Plasmid

   #162164

   Purpose

   Selectable homology donor template for Aag2 cell AGO1

   Depositor

   Charles Rice

   Article

   Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

   Inserts

   RFP
   3xFLAG
   

   Use

   CRISPR and Cre/Lox; Homology donor template

   Tags

   AGO1 homology and loxP

   Expression

   Insect

   Mutation

   Promoter

   Ae. aegypti PUb and endogenous AGO1

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPapi

    Plasmid

    #96921

    Purpose

    expression of S aureus SaCas9 (SauCas9) and two pol III promoters for an Sa gRNA and an Sp gRNA. Intended to be used in SpCas9-expressing cell lines for dual Cas9 "Big Papi" screens. aka pXPR207.

    Depositor

    John Doench, David Root

    Article

    Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-ABE-P48R

    Plasmid

    #161816

    Purpose

    Expresses ABE-P48R in mammalian cells

    Depositor

    Sangsu Bae

    Insert

    bpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAVS1-SAGFP

    Plasmid

    #127100

    Purpose

    SA-GFP substrate (Addgene #41594) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. SA-GFP cassette contains PGK-puromycin.

    Depositor

    Adam Karpf

    Insert

    SA-GFP reporter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGRB

    Plasmid

    #71539

    Purpose

    for expression of gRNA in E. coli

    Depositor

    Tao Chen

    Article

    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J231196

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLX-sgRNA

    Plasmid

    #50662

    Purpose

    Lentiviral expression of AAVS1-targeting sgRNA; insert can be replaced with custom sgRNA (see protocol)

    Depositor

    Eric Lander, David Sabatini

    Article

    Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

    Insert

    sgAAVS1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSQT817

    Plasmid

    #53373

    Purpose

    wildtype Cas9 expression plasmid

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGGDestTol2LC-5sgRNA

    Plasmid

    #64243

    Purpose

    destination vector for five U6x:sgRNA cassettes

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGGDestTol2LC-4sgRNA

    Plasmid

    #64242

    Purpose

    destination vector for four U6x:sgRNA cassettes

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGGDestTol2LC-2sgRNA

    Plasmid

    #64240

    Purpose

    destination vector for two U6x:sgRNA cassettes

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGGDestTol2LC-3sgRNA

    Plasmid

    #64241

    Purpose

    destination vector for three U6x:sgRNA cassettes

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGGDestTol2LC-1sgRNA

    Plasmid

    #64239

    Purpose

    destination vector for one U6x:sgRNA cassette

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. AP588-1

    Plasmid

    #71278

    Purpose

    Expresses myc::TEV::mTagGFP::TEV::3Xflag in bacteria

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

    Insert

    tagGFP
    

    Use

    Tags

    TEV::3xFlag and myc::TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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22. AP625-1

    Plasmid

    #70051

    Purpose

    Expresses myc::TEV::3Xflag::eGFP::3Xflag::TEV::myc in bacteria

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

    Insert

    eGFP
    

    Use

    Tags

    3Xflag::TEV::myc and myc::TEV::3Xflag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCB-CRISPRi

    Plasmid

    #221136

    Purpose

    Coxiella burnetii CRISPRi plasmid without sgRNA construct. Expresses 3xF-dCas9.

    Depositor

    Craig Roy

    Article

    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

    Insert

    3xF-dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    cbu1169 promoter

    Available since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. pCMV-T7-AcrIIA1-Leu-NLS(SV40) (KAC440)

    Plasmid

    #133799

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized Leu-AcrIIA1 with C-terminal NLS (SV40)

    Depositor

    Benjamin Kleinstiver, Joseph Bondy-Denomy

    Article

    Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

    Insert

    human codon optimized Leu-AcrIIA1
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CMV and T7

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMV-T7-AcrIIA1-Eriv-NLS(SV40) (KAC442)

    Plasmid

    #133800

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized Eriv-AcrIIA1 with C-terminal NLS (SV40)

    Depositor

    Benjamin Kleinstiver, Joseph Bondy-Denomy

    Article

    Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

    Insert

    human codon optimized Eriv-AcrIIA1
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CMV and T7

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV-T7-AcrIIA1(T16A)-NLS(SV40) (KAC511)

    Plasmid

    #133797

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1(T16A) with C-terminal NLS (SV40)

    Depositor

    Benjamin Kleinstiver, Joseph Bondy-Denomy

    Article

    Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

    Insert

    human codon optimized AcrIIA1(T16A)
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    T16A

    Promoter

    CMV and T7

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJEP10-AAV-U6/TO-gRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid

    #82706

    Purpose

    AAV backbone with a full length U6/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl sites. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.

    Depositor

    Jonathan Ploski

    Article

    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do

    Insert

    U6/TO Empty gRNA Expression Cassette
    

    Use

    AAV and CRISPR

    Tags

    CMV promoter driving expression of TetR-P2A-GFP-K…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJEP12-AAV-H1/TO-L-sgRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid

    #82704

    Purpose

    AAV backbone with a full length H1/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl sites. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.

    Depositor

    Jonathan Ploski

    Article

    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do

    Insert

    H1/TO-L Empty gRNA Expression Cassette
    

    Use

    AAV and CRISPR

    Tags

    CMV promoter driving expression of TetR-P2A-GFP-K…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJEP14-AAV-H1/TO-sgRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid

    #82702

    Purpose

    AAV backbone with a minimal H1/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.

    Depositor

    Jonathan Ploski

    Article

    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do

    Insert

    H1/TO Empty gRNA Expression Cassette
    

    Use

    AAV and CRISPR

    Tags

    CMV promoter driving expression of TetR-P2A-GFP-K…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. HF-PX459 (V2)

    Plasmid

    #118632

    Purpose

    Plasmid encoding SpCas9-HF1, a single guide RNA and puromycin resistance

    Depositor

    Mike McGrew

    Article

    Idoko-Akoh et al Sci Rep. 2018 Oct 11;8(1):15126. doi: 10

    Insert

    hSpCas9-HF1-2A-Puro V2.0
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Asparagine 497 to Alanine, Arginine 661 to Alanin…

    Promoter

    Cbh

    Available since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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