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Showing: 2481 - 2520 of 5006 results

  2. pCEP4-SERTI425V-myc

    Plasmid
    #107464
    Purpose
    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation I425V conferring aproximately wildtype phenotype
    Depositor
    Erik Procko
    Article
    Young et al bioRxiv 2021.04.19.440442
    Insert
    Serotonin Transporter (SERT) (SLC6A4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    I425V
    Promoter
    CMV
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    107464-plasmid-map-sequence-id-205573

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  3. lentiCRISPR - C3orf17 sgRNA 1

    Plasmid
    #70652
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C3orf17 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    David Sabatini
    Article
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against C3orf17
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  4. lentiCRISPR - C9orf114 sgRNA 1

    Plasmid
    #70655
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C9orf114 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    David Sabatini
    Article
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against C9orf114
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  5. lentiCRISPR - C9orf114 sgRNA 2

    Plasmid
    #70654
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C9orf114 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    David Sabatini
    Article
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against C9orf114
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  10. pME_Cp_0_4_035_aphA6-Kan

    Plasmid
    #235904
    Purpose
    Aminoglycoside-3'-phosphotransferase selection gene, codon optimized for Chlamydomonas reinhardtii
    Depositor
    Tobias Erb
    Insert
    aphA6-Kann
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    235904-plasmid-map-sequence-id-471485

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  11. pME_Cp_0_4_008_tdTomato

    Plasmid
    #235895
    Purpose
    tdTomato reporter gene, codon optimized for Chlamydomonas reinhardtii
    Depositor
    Tobias Erb
    Insert
    tdTomato
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    235895-plasmid-map-sequence-id-471481

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  12. pME_Cp_0_4_024_CrCD-marker

    Plasmid
    #235900
    Purpose
    Cytosine deaminase selection gene, codon optimized for Chlamydomonas reinhardtii
    Depositor
    Tobias Erb
    Insert
    CrCD-marker
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    235900-plasmid-map-sequence-id-471483

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  13. pME_Cp_0_4_026_Arg9-marker

    Plasmid
    #235901
    Purpose
    N-acetyl ornithine aminotransferase selection gene, codon optimized for Chlamydomonas reinhardtii
    Depositor
    Tobias Erb
    Insert
    Arg9-marker
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    235901-plasmid-map-sequence-id-471484

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  14. pME_Cp_0_4_001_Nanoluc

    Plasmid
    #235893
    Purpose
    Nanoluc luciferase reporter gene, codon optimized for Chlamydomonas reinhardtii
    Depositor
    Tobias Erb
    Insert
    NanoLuc
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    235893-plasmid-map-sequence-id-471479

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  15. pME_Cp_0_4_007_mScarlet-I

    Plasmid
    #235894
    Purpose
    mScarlet-I reporter gene, codon optimized for Chlamydomonas reinhardtii
    Depositor
    Tobias Erb
    Insert
    mScarlet-I
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    235894-plasmid-map-sequence-id-471480

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  26. pET28-NFLtail

    Plasmid
    #232055
    Purpose
    Expresses construct for surface tethering: R. norvegicus NFL tail domain
    Depositor
    Sanjay Kumar
    Article
    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121
    Insert
    Neurofilament-Light tail domain
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    cDNA codon-optimized for E. coli; added N-termina…
    Promoter
    T7
    Available since
    March 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    232055-plasmid-map-sequence-id-464065

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  28. pCAG-memRFP-3xControlgRNA

    Plasmid
    #224567
    Purpose
    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter.
    Depositor
    Erica Hutchins
    Article
    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026
    Insert
    mRFP1
    Use
    CRISPR
    Tags
    Membrane localization signal
    Expression
    Mutation
    Codon optimized
    Promoter
    Available since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    224567-plasmid-map-sequence-id-451479

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  29. pCAG-3xGFP11-mem-3xControlgRNA

    Plasmid
    #224583
    Purpose
    Ubiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.
    Depositor
    Erica Hutchins
    Article
    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026
    Insert
    3xGFP11
    Use
    CRISPR
    Tags
    Membrane Localization Signal
    Expression
    Mutation
    Codon optimized
    Promoter
    Available since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    224583-plasmid-map-sequence-id-451495

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  30. GB3774

    Plasmid
    #215247
    Purpose
    CDS of CPH from N. nambi codon optimized for N.benthamiana. This gene is the final step and recycling of the bioluminiscente pathway
    Depositor
    Diego Orzaez
    Article
    Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47
    Insert
    CPH
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Oct. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    215247-plasmid-map-sequence-id-447291

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  31. pDONR221_zCas9

    Plasmid
    #213913
    Purpose
    Entry clone containing 3×FLAG-NLS-zCas9-NLS encoding sequence, Pisum sativum rbcS E9 terminator. For use in plants and compatible with the MultiSite Gateway system
    Depositor
    Sébastien Mongrand
    Article
    Li et al Bio Protoc. 2024 Jul 5;14(13):e5029. doi
    Insert
    zCas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    Available since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    213913-plasmid-map-sequence-id-437263

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  32. GB3474

    Plasmid
    #215370
    Purpose
    CDS of CPH from N. nambi codon optimized for N.benthamiana. This gene is the final step and recycling of the bioluminiscente pathway
    Depositor
    Diego Orzaez
    Article
    Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47
    Insert
    CPH
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    June 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    215370-plasmid-map-sequence-id-437491

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  33. GB3473

    Plasmid
    #215246
    Purpose
    CDS of Luz gene from N. nambi codon optimized for N.benthamiana. This gene generate the luminiscence of the bioluminiscente pathway
    Depositor
    Diego Orzaez
    Article
    Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47
    Insert
    Luz
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    June 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    215246-plasmid-map-sequence-id-436566

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  34. GB3472

    Plasmid
    #215245
    Purpose
    CDS of H3H from N. nambi codon optimized for N.benthamiana. This gene is the second step of the bioluminiscente pathway
    Depositor
    Diego Orzaez
    Article
    Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47
    Insert
    H3H
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    June 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    215245-plasmid-map-sequence-id-436564

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  38. NK809

    Plasmid
    #196406
    Purpose
    Strong expression of firefly and nanoluc luciferases codon-optimized for S. rosetta
    Depositor
    Nicole King
    Article
    Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092
    Insert
    Sro5EFL-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
    Use
    Luciferase
    Tags
    Expression
    Mutation
    N/A (synthetic)
    Promoter
    Available since
    Aug. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    196406-plasmid-map-sequence-id-385017

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Showing: 2481 - 2520 of 5006 results