We narrowed to 12,292 results for: BASE

1. Dimer 42 AA

   Plasmid

   #198198

   Purpose

   FRET calibration: Dimer construct consisting of mTurquoise2 and mVenus with a 42AA linker

   Depositor

   Christian Wahl-Schott

   Article

   Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

   Insert

   mVenus-42AA-mTurquoise2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Dimer 3 AA

   Plasmid

   #198197

   Purpose

   FRET calibration: Dimer construct consisting of mTurquoise2 and mVenus with a 3AA linker

   Depositor

   Christian Wahl-Schott

   Article

   Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

   Insert

   mVenus-3AA-mTurquoise2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. SpCas9-CBE6c-IVT

   Plasmid

   #215835

   Purpose

   Template for in vitro transcription of CBE6c (with SpCas9)

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6c-SpCas9-2xUGI
   

   Use

   In vitro transcription template

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAS9-mCherry-Frame +2

   Plasmid

   #66941

   Purpose

   CRISPaint frame selector +2

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Insert

   gRNA frame +2
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCRISPR-dCas9

   Plasmid

   #125687

   Purpose

   Gene knockdown for actinomycetes

   Depositor

   Tilmann Weber

   Article

   Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

   Insert

   streptomyces codon optimized spdCas9, sgRNA casette
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   ermE*/tipA

   Available since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pnsgRNA

   Plasmid

   #172717

   Purpose

   Delivery of guide RNA for prime editing

   Depositor

   Tilmann Weber

   Article

   Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

   Insert

   J23119-gRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHR SFFVp Phy-mCherry-CAAX

   Plasmid

   #50839

   Purpose

   Optogenetic PI3K activation system; membrane component

   Depositor

   Wendell Lim

   Article

   Toettcher et al Nat Methods. 2011 Sep 11;8(10):837-9. do

   Insert

   PhyB 1-908
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Feb. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Green Glifon4000

   Plasmid

   #126208

   Purpose

   green fluorescent glucose sensor for mammalian cells

   Depositor

   Tetsuya Kitaguchi

   Article

   Mita et al Anal Chem. 2019 Apr 2;91(7):4821-4830. d

   Insert

   Green Glifon4000
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFREE_Cm

   Plasmid

   #92052

   Purpose

   Allows inducible expression of gRNAs and Cas9 for plasmid curing.

   Depositor

   Morten Norholm

   Article

   Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

   Inserts

   Cas9
   gRNA array
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   pRhamBAD and ptet

   Available since

   Aug. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pmMaple3-CAM

    Plasmid

    #101148

    Purpose

    Fluorescent protein for superresolution imaging

    Depositor

    Xiaowei Zhuang

    Article

    Wang et al Proc Natl Acad Sci U S A. 2014 Jun 10;11

    Insert

    E. coli codon optimized mMaple3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-Ptet-RFP-shR-rtTA

    Plasmid

    #35625

    Purpose

    Tet-inducible AAV shRNA vector to test efficacy of shRNAs. Used in combination with pGFPns-reporter (Addgene plasmid #35626).

    Depositor

    Howard Gu

    Article

    Naughton et al Anal Biochem. 2011 Oct 1;417(1):162-4. E

    Type

    Empty backbone

    Use

    AAV and RNAi; Tet inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ptet

    Available since

    April 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDV05.0_pEF1a-iRFP720

    Plasmid

    #200407

    Purpose

    Expresses iRFP720 constitutively in mammalian cells

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    iRFP720
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLVX-EF1a-PTS1-IRES-Puromycin

    Plasmid

    #134665

    Purpose

    Expresses EGFP-tagged peroxisome marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    tripeptide SKL (Peroxisome targeting signal 1)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHSV-TK

    Plasmid

    #165801

    Purpose

    Minimal viral terminator derived from Herpes Simplex 1 thymidine kinase gene

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    HSV-TK
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET24b-BG1.3

    Plasmid

    #199437

    Purpose

    expresses BAmGreen1.3 in bacterial cells

    Depositor

    Andrew Woolley

    Article

    Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

    Insert

    BAMGreen1.3
    

    Use

    Tags

    his tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CellTagA

    Plasmid

    #124591

    Purpose

    Expressed barcode for sample multiplexing in single-cell analysis

    Depositor

    Samantha Morris

    Article

    Guo et al Genome Biol. 2019 May 9;20(1):90. doi: 1

    Insert

    CellTagA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GAL4UAS-Luciferase reporter

    Plasmid

    #64125

    Purpose

    Photoactivatable transcription system. Luciferase reporter under the control of the upstream activator sequence (UAS) of Gal4.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    GAL4UAS
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    GAL4UAS

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p667-UBC-CDK12-V5_IDG-K

    Plasmid

    #135245

    Purpose

    Gateway destination clone of CDK12 (human) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    CDK12-V5 (CDK12 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFA6a-3HA-TRP1

    Plasmid

    #41599

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    TRP1 (TRP1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    3xHA and yeast ADH1 terminator

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMU-ERr

    Plasmid

    #61170

    Purpose

    Fluorescent reporter for endoplasmic reticulum AtWAK2sp-mCherry-HDEL expressed under AtUBQ10 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    AtWAK2-mCherry-HDEL
    

    Use

    Tags

    mCherry

    Expression

    Plant

    Mutation

    Promoter

    AtUBQ10

    Available since

    April 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSFVCs-lacZ

    Plasmid

    #92076

    Purpose

    This is a high level expression plasmid for production of recombinant genomic SFV RNA encoding a C-beta galactosidase fusion protein.

    Depositor

    Henrik Garoff

    Article

    Sjoberg et al Biotechnology (N Y). 1994 Nov;12(11):112

    Insert

    C-lacZ
    

    Use

    C-lacz expression plasmid, for run-off transcript…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET24b-BR1.4

    Plasmid

    #199436

    Purpose

    expresses BAmRed1.4 in bacterial cells

    Depositor

    Andrew Woolley

    Article

    Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

    Insert

    BAMRed1.4
    

    Use

    Tags

    his tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDisplay-R-iGluSnFR1

    Plasmid

    #107335

    Purpose

    Red fluorescent glutamate sensor based on cpmApple. Membrane-displayed

    Depositor

    Robert Campbell

    Article

    Wu et al ACS Chem Biol. 2018 Jan 22. doi: 10.1021

    Insert

    R-iGluSnFR1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Green Glifon50

    Plasmid

    #126206

    Purpose

    green fluorescent glucose sensor for mammalian cells

    Depositor

    Tetsuya Kitaguchi

    Article

    Mita et al Anal Chem. 2019 Apr 2;91(7):4821-4830. d

    Insert

    Green Glifon50
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCN38-yfp

    Plasmid

    #227637

    Purpose

    Negative control plasmid encoding yfp downstream of an attenuated ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.

    Depositor

    Angelika Grundling

    Article

    Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

    Insert

    yfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMU-TPr

    Plasmid

    #61189

    Purpose

    Fluorescent reporter for tonoplast AtTIP1-1-mCherry expressed under AtUBQ10 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    AtTIP1-1
    

    Use

    Tags

    mCherry

    Expression

    Plant

    Mutation

    Promoter

    AtUBQ10

    Available since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pESC-URA (V5 and HA)

    Plasmid

    #222485

    Purpose

    Co-expression of two genes in yeast with V5 and HA tags and URA3 selection

    Depositor

    Kiyofumi Yamada, Tetsuo Matsuzaki

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    HA and V5

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pYSEAP

    Plasmid

    #37326

    Depositor

    John Schiller

    Article

    Pastrana et al Virology. 2004 Apr 10;321(2):205-16.

    Insert

    SEAP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLN-AIDA-Tyr1

    Plasmid

    #192831

    Purpose

    IPTG inducible AIDA-Tyrosinase expression

    Depositor

    André Studart

    Article

    Binelli et al Adv Mater. 2022 Nov 29:e2207483. doi: 10

    Insert

    AIDA-Tyr1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pL-lac

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBI-MCS-EYFP

    Plasmid

    #58855

    Purpose

    The tet-responsive reporter pBI-MCS-EGFP (Addgene plasmid 16542) was modified to express YFP instead of GFP

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    enhanced yellow fluorescent protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. H3K9me3Probe_FullLength

    Plasmid

    #186463

    Purpose

    Expresses GFP tagged H3K9me3 recognition motif in mammalian cells.

    Depositor

    Chongli Yuan

    Article

    Mendonca et al Cytometry A. 2022 Apr;101(4):339-350. do

    Insert

    dimeric CDY1 (CDY1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pFA6a-13Myc-TRP1

    Plasmid

    #41601

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    TRP1 (TRP1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    13Myc and yeast ADH1 terminator

    Expression

    Mutation

    Promoter

    Available since

    Dec. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. MCP-APEX2

    Plasmid

    #154936

    Purpose

    NLS-V5-MCP-APEX2

    Depositor

    Alice Ting

    Article

    Han et al Proc Natl Acad Sci U S A. 2020 Aug 24. p

    Insert

    NLS-V5-MCP-APEX2
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. p663-UBC-miniTurbo-V5-CDK14_IDG-K

    Plasmid

    #158172

    Purpose

    Gateway destination clone of CDK14 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-CDK14 (CDK14 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pRPLC

    Plasmid

    #122261

    Purpose

    pBRBAD-rbsD-RFP-B0015- B0015-LasR-rbs34-J23108-pBRlasI-rbsD-RFP- B0015

    Depositor

    Chueh Loo Poh

    Article

    Jayaraman et al ACS Synth Biol. 2018 Nov 16;7(11):2627-2

    Insert

    pBRBAD, pBRlasI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pESC-ADE (V5 and HA)

    Plasmid

    #222489

    Purpose

    Co-expression of two genes in yeast with V5 and HA tags and ADE2 selection

    Depositor

    Kiyofumi Yamada, Tetsuo Matsuzaki

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    HA and V5

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3-ClopHensor

    Plasmid

    #25938

    Depositor

    Daniele Arosio

    Article

    Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

    Insert

    ClopHensor
    

    Use

    Tags

    Strep-II

    Expression

    Mammalian

    Mutation

    E2GFP is EGFP-T203Y

    Promoter

    Available since

    Sept. 13, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pU6-GG-gRNA

    Plasmid

    #226860

    Purpose

    Human gRNA expression vector backbone with BsmBI Golden Gate site

    Depositor

    Alexis Komor

    Article

    Osgood et al Methods in Enzymology, February 5, 2025

    Insert

    Sp-gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. VKG1-gRNA-pX330

    Plasmid

    #108671

    Purpose

    Cleavage of VKG1 sequence by CRISPR/Cas9

    Depositor

    Shigeo Sugano

    Article

    Sawatsubashi et al Sci Rep. 2018 Jan 12;8(1):593. doi: 10.1

    Insert

    gRNA for VKG1 sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. MSCV-BCR-ABL1-p190-IRES-EGFP

    Plasmid

    #219964

    Purpose

    Express human BCR-ABL1-P190 in mammalian cells

    Depositor

    Mohamed Fareh

    Insert

    BCR-ABL1-P190 (ABL1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge