We narrowed to 17,993 results for: puro

Showing: 1261 - 1280 of 17993 results

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pCW-Cdt1-mCherry-Puro

Plasmid

#193763

Purpose

Doxycyline-inducible (TetOn) human Cdt1 (C-terminal mCherry tag), expressed from all-in-one pCW vector (TetOn + rtTA)

Depositor

Tobias Meyer

Article

Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

Insert

Cdt1-mCherry (CDT1 Human)

Use

Lentiviral

Tags

WT

Mutation

human Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…

Promoter

TRE

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-BE4GamRA-P2A-Puro

Plasmid

#112673

Purpose

Lentivirus for constitutive expression of BE4GamRA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE4GamRA

Use

Lentiviral

Tags

FLAG

Mutation

NLS sequence at the N-terminus and D10A

Promoter

EF1s

Available Since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Puro-GFPg1 (BB09)

Plasmid

#139461

Purpose

Lentiviral vector with gRNA targeting GFP; includes puromycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

GFP-targeting sgRNA inserted; resistance gene: puroR

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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Tet-pLKO-puro-shSF3B1

Plasmid

#248800

Purpose

Knockdown of endogenous SF3B1 with shRNA targeting 3'UTR

Depositor

Maayan Salton

Article

Baker et al RNA. 2025 Dec 24:rna.080661.125. doi: 10

Insert

shRNA targeting SF3B1 3'UTR

Use

Lentiviral

Promoter

hPGK

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCDH-3xFLAG-GFP-puroR

Plasmid

#167463

Purpose

3rd-gen lentiviral expression of FLAG-GFP in mammalian cells

Depositor

Steven Artandi

Article

Chen et al Cell. 2018 Jun 28;174(1):218-230.e13. do

Insert

GFP

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP

Plasmid

#102796

Purpose

Retrovirus for delivery of one sgRNA (empty, bbsI)

Depositor

Sarah Teichmann

Article

Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

Insert

U6 sgRNA

Use

CRISPR and Retroviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-Puro (PX462) V2.0

Plasmid

#62987

Purpose

Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9n-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Cas9 D10A nickase mutant

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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LentiGuide-puro-NLS-mCherry

Plasmid

#185474

Purpose

Mammalian Expression, Lentiviral vector expressing mCherry for two-colour competitive growth assay. Clone guide oligos into BsmBI site.

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Type

Empty backbone

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(BbsI)-PGKpuro2AmCherry-W

Plasmid

#67977

Purpose

CRISPR gRNA expression vector with an improved scaffold and puro/mCherry markers

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKpuro2AmCherry cassette, WPRE

Use

Lentiviral

Mutation

Deleted BbsI site within WPRE

Promoter

human U6 promoter, mouse PGK promoter

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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Tet-pLKO-puro-Scrambled

Plasmid

#47541

Purpose

Tet inducible scrambled shRNA.

Depositor

Charles Rudin

Article

Rudin et al Nat Genet. 2012 Oct;44(10):1111-6. doi:

Insert

Scrambled shRNA

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase

Plasmid

#119816

Purpose

3rd generation lentiviral plasmid for expression of EGFP Firefly Luciferase fusion protein in mammalian cells

Depositor

Ethan Abel

Article

Lorenzatti Hiles et al Nat Protoc. 2019 Mar;14(3):738-755. doi:

Inserts

Luciferase
Puromycin

Use

Lentiviral and Luciferase

Tags

EGFP and V5

Expression

Mammalian

Mutation

Stop codon removed in the frame of the V5 frame v…

Promoter

CMV/TO and Human Phosphoglycerate kinase

Available Since

Jan. 2, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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EF1a-ABCD3-RFP-Puro

Plasmid

#170168

Purpose

Mammalian Expression of Ef1a-ABCD3-RFP-Puro

Depositor

Christopher Moxham

Insert

ABCD3-RFP-Puro

Expression

Mammalian

Mutation

n/a

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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LentiGuide-puro-NLS-GFP

Plasmid

#185473

Purpose

Mammalian Expression, Lentiviral vector expressing GFP for two-colour competitive growth assay. Clone guide oligos into BsmBI site.

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Type

Empty backbone

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pMXs-Puro-CLDN18-ARHGAP26

Plasmid

#69465

Purpose

Expresses Cldn18-Arhgap26 fusion gene.

Depositor

Axel Hillmer

Article

Yao et al Cell Rep. 2015 Jul 14;12(2):272-85. doi:

Insert

CLDN18-ARHGAP26 (ARHGAP26, CLDN18 Human)

Use

Retroviral

Tags

HA TAG

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pCW-myc-YAP5SA-puro

Plasmid

#249725

Purpose

Doxycycline-inducible expression of myc epitope-tagged YAP5SA with puro selection

Depositor

Tobias Meyer

Article

Ferrick et al bioRxiv [Preprint]. 2024 Nov 4:2024.10.1

Insert

YAP5SA (YAP1 Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Mutation

S61A, S109A, S127A, S164A, S381A (phosphorylation…

Promoter

Tet ON

Available Since

Feb. 9, 2026

Availability

Academic Institutions and Nonprofits only

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pLD-puro-MTS-EGFP

Plasmid

#190270

Purpose

Lentiviral conditional expression of mitochondrial-targeted eGFP in mammalian cells

Depositor

Christian Münch

Article

Michaelis et al Bio Protoc. 2022 Dec 20;12(24):e4578. do

Insert

MTS-EGFP

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

Tight TRE promoter

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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Tet-pLKO-puro-SOX2

Plasmid

#47540

Purpose

Tet inducible knockdown of SOX2.

Depositor

Charles Rudin

Article

Rudin et al Nat Genet. 2012 Oct;44(10):1111-6. doi:

Insert

SOX2 shRNA

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-mNeon-PuroR [M1G]

Plasmid

#171800

Purpose

Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and puromycin resistance cassette [p.M1G]

Depositor

Michael Hoelzel

Article

Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

Insert

mNeonGreen-T2A-PuroR

Use

Gene tagging

Mutation

PuroR: Changed Methionin 1 to Glycine.

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH-CMV-CD19 puro

Plasmid

#196634

Purpose

Express CD19

Depositor

Nicolas Manel

Article

Jeremiah et al J Exp Med. 2023 May 1;220(5):e20220666.

Insert

CD19 (CD19 Human)

Use

Lentiviral

Mutation

WT

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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P3-dCas9-Tet1-GFP-Puro

Plasmid

#190728

Purpose

Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)

Depositor

Maja Jagodic

Article

Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:

Insert

dCas9-tet1

Use

CRISPR

Tags

3XFLAG and SV40 NLS

Expression

Mammalian

Mutation

Changed Pro434 codon from CCG to CCC and Gly468 f…

Promoter

pCAG

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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