-
Plasmid#222986PurposeTo express a putative signal peptide from Encephalitozoon intestinalis protein Eint_061510 fused to GFP in mammalian cellsDepositorInsertsfGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
V5-WT-short-UBASH3A
Plasmid#192088PurposeMammalian expression of human wild-type short-form UBASH3A with V5 tagDepositorInsertUBASH3A (UBASH3A Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV-10xQb oligo-library based
Plasmid#158201PurposeCassette of 10 different binding sites with high affinity to QB phage coat protein, as extracted from the oligo pool experiment.DepositorInsert10 binding sites of QB based on oligo library experiment
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC5Kan-P2A
Plasmid#51814PurposeP2A peptide from porcine teschovirus-1 flanked by restriction sites in shuttle vector for easy cloning of multiple separate proteins to be expressed from single messageDepositorInsertP2A peptide (PTV1gp1 Synthetic, porcine teschovirus-1)
UseShuttle vector for insect expression vectorsTagsExpressionMutationcodons altered for Drosophila and to eliminate re…PromoterAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
CMV-10xPP7 oligo-library based
Plasmid#158199PurposeCassette of 10 different binding sites with high affinity to PP7 phage coat protein, as extracted from the oligo pool experiment.DepositorInsert10 binding sites of PP7 from the oligo library
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-10xMS2 oligo-library based
Plasmid#158200PurposeCassette of 10 different binding sites with high affinity to MS2 phage coat protein, as extracted from the oligo pool experiment.DepositorInsert10 binding sites of MS2 from oligo library experiment
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-10xPP7-Qb model based
Plasmid#158203PurposeCassette of 10 different binding sites with high affinity to both PP7 and Qb phage coats protein, as computed by the ML model.DepositorInsert10 binding sites with high affinity to PP7 and QB coat proteins based on the machine-learning model
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-10xMS2 no wild type
Plasmid#158204PurposeCassette of 10 different binding sites with high affinity to MS2 phage coat protein, as extracted form the oligo pool experiment. It does not contain the MS2-wt sequence, therefore QCP cannot bind it.DepositorInsert10 binding sites of MS2 without the WT sequence from oligo library experiment
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-10xQb no wild type
Plasmid#158205PurposeCassette of 10 different binding sites with high affinity to Qb phage coat protein, as extracted form the oligo pool experiment. It does not contain the Qb-wt sequence, therefore MCP cannot bind it.DepositorInsert10 binding sites of QB coat protein without the WT sequence based on the oligo library experiment
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
phDLX2-N174
Plasmid#60860Purpose2nd generation lentiviral transfer plasmid. Expresses DLX2 under the EF1a promoterDepositorInsertDLX2 (DLX2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_SIK3_CR_T221E
Plasmid#108108PurposeLentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & TE mutation at LKB1 phosphorylation site) with neomycin resistance geneDepositorInsertSIK3 (SIK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationchange guanine 501 to cytosine (silent mutation),…PromoterEFS promoterAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
LV-hGFAP-DLX2-ires-GFP
Plasmid#213602PurposeExpression of human DLX2 and GFP under the human GFAP ABC1D promoterDepositorInsertDLX2 (DLX2 Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNT-1
Plasmid#154935Purpose1 designed non-target siteDepositorInsert1 designed non-target sites
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNJB91
Plasmid#51519PurposeGateway entry vector with attL1 and attR5 sites. Between the att sites are two FokI coding sequences transcriptionally linked by a T2A sequence. There is a nos-T after the last FokI.DepositorTypeEmpty backboneUseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceMarch 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
NK651
Plasmid#109107PurposeReporter gene expression in S. rosettaDepositorInsertPTSG_07215 deltaCC
UseChoanoflagellate expressionTagsExpressionMutationDeletes C-terminal coiled-coil from PTSG_07215PromoterAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS-TarHom-Tol2-TetR-cryaa-YFP
Plasmid#73202PurposeBAC targeting cassette for pTARBAC2 with tetR selection marker and lens-specific transgenesis marker CitrineDepositorInsertYFP
UseTagsExpressionMutationPromoterAvailable sinceMarch 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
PCT62
Plasmid#170391Purposeexpresses three sgRNA cassettesDepositorInsertPCT62-targetsite
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-TarHom-Tol2-kanaR-cryaa-CFP
Plasmid#74151PurposeBAC targeting cassette for pTARBAC2 with kanaR selection marker and lens-specific transgenesis marker CeruleanDepositorInsertTol2-kanaR-cryaa-CFP
UseTagsExpressionMutationPromoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS-TarHom-Tol2-kanaR-cryaa-dsRed
Plasmid#74152PurposeBAC targeting cassette for pTARBAC2 with kanaR selection marker and lens-specific transgenesis marker dsRedDepositorInsertTol2-kanaR-cryaa-dsRed
UseTagsExpressionMutationPromoterAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS-TarHom-Tol2-kanaR-cryaa-sfGFP
Plasmid#74153PurposeBAC targeting cassette for pTARBAC2 with kanaR selection marker and lens-specific transgenesis marker sfGFPDepositorInsertTol2-kanaR-cryaa-sfGFP
UseTagsExpressionMutationPromoterAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only