20,039 results

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pCold-I-Dsup

Plasmid

#90021

Purpose

To express His-tagged Dsup in bacteria

Depositor

Takekazu Kunieda

Article

Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

Insert

Dsup

Tags

His

Expression

Bacterial

Promoter

cspA

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pMBIS

Plasmid

#17817

Depositor

Jay Keasling

Article

Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

Insert

mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase, FPP synthase

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 27, 2008

Availability

Academic Institutions and Nonprofits only

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pLL3.7m-Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)

Plasmid

#83841

Purpose

Fluorescent probe for G1/S transition and M/G1 transition, one of two plasmids for the FUCCI4 cell cycle reporter

Depositor

Michael Lin

Article

Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120) (GMNN, CDT1 Human, Synthetic)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pVSV eGFP dG

Plasmid

#31842

Depositor

Connie Cepko

Article

Beier et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

Insert

VSV genome

Expression

Bacterial

Available Since

Oct. 1, 2012

Availability

Academic Institutions and Nonprofits only

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dCas9-KRAB-MeCP2

Plasmid

#110821

Purpose

Improved dCas9 repressor-dCas9-KRAB-MeCP2

Depositor

Alejandro Chavez, George Church

Article

Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

Insert

dCas9-KRAB-MeCP2

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Promoter

CMV

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pUC18R6K-mini-Tn7T-Gm

Plasmid

#65022

Purpose

GmR mini-Tn7 vector, R6K replicon

Depositor

Herbert Schweizer

Article

Choi et al Nat Protoc. 2006;1(1):153-61.

Type

Empty backbone

Available Since

Aug. 17, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-GFP11-Clathrin light chain

Plasmid

#70217

Purpose

Expresses GFP11-clathrin light chain in mammalian cells

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

GFP11-Clathrin light chain (CLTA Human)

Tags

GFP11

Expression

Mammalian

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pRK793

Plasmid

#8827

Depositor

David Waugh

Article

Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

Insert

TEV protease, S219V mutant

Tags

His, MBP (with TEV), and polyarginine

Expression

Bacterial

Mutation

S219V mutation (improves stability)

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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pLX_311-KRAB-dCas9

Plasmid

#96918

Purpose

for CRISPRi, lentiviral expression of KRAB-dCas9 and BlastR. Also called pXPR_121

Depositor

John Doench, William Hahn, David Root

Article

Rosenbluh et al Nat Commun. 2017 May 23;8:15403. doi: 10

Insert

dCas9

Use

Lentiviral

Tags

KRAB

Expression

Mammalian

Mutation

dCas9

Promoter

EF1a

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCBh-NLS_hfCas13d(RfxCas13d_N2V8)_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

Plasmid

#190034

Purpose

vector for encoding a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP and mCherry driven by SV40 promoters

Depositor

Huawei Tong

Article

Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

Insert

humanized hfCas13d

Expression

Mammalian

Mutation

A134V,A140V,A141V,A143V

Promoter

CBh, SV40, hU6

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pSFFV_mNG3A(1-10)

Plasmid

#157992

Purpose

Lentiviral vector of constitutive expression of mNeonGreen3A(1-10)

Depositor

Bo Huang

Article

Zhou et al PLoS One. 2020 Nov 23;15(11):e0242592. d

Insert

pSFFV_mNG3K(1-10)

Use

Lentiviral

Expression

Mammalian

Mutation

W148L, M150V, K153M, N194K, F204L

Promoter

SFFV

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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hTLR4

Plasmid

#13086

Depositor

Ruslan Medzhitov

Insert

TLR4 (TLR4 Human)

Expression

Mammalian

Available Since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only

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pAAV.GfaABC1D.PI.Lck-GFP.SV40

Plasmid

#105598

Purpose

AAV expression of membrane bound EGFP from GfaABC1D promoter

Depositor

Baljit Khakh

Article

Shigetomi et al J Gen Physiol. 2013 Apr 15.

Has Service

AAV5

Insert

Lck-EGFP

Use

AAV

Expression

Mammalian

Promoter

GfaABC1D

Available Since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST-hMeCP2-GFP

Plasmid

#48078

Purpose

Mammalian expression of GFP tagged MeCP2-WT

Depositor

Huda Zoghbi

Article

Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

Insert

Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)

Tags

GFP

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

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pLS-SceI

Plasmid

#137725

Purpose

Lentivirus reporter assay plasmid that contains a I-SceI site and a EGFP reporter gene.

Depositor

Nadav Ahituv

Article

Gordon et al Nat Protoc. 2020 Aug;15(8):2387-2412. do

Type

Empty backbone

Use

Lentiviral

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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lenti EF1a-FLAG-dCas9-VPR

Plasmid

#114195

Purpose

Lentivirus compatible dCas9-VPR construct driven by the EF1a promoter

Depositor

Jeremy Day

Article

Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

Insert

dCas9-VPR

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti Lifeact-mTagBFP2 PuroR

Plasmid

#101893

Purpose

Lentiviral expression of mTagBFP2-tagged Lifeact with Puromycin selection in cells including neurons

Depositor

Ghassan Mouneimne

Insert

Lifeact

Use

Lentiviral

Tags

mTagBFP2

Promoter

CMV immediate early

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-UFM1

Plasmid

#134639

Purpose

expression of HA-tagged human UFM1 wildtype

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

UFM1 (UFM1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pRRL-MHCK7-GCaMP6

Plasmid

#65042

Purpose

Lentiviral vector expressing the calcium indicator GCaMP6 driven by the muscle-specific promoter MHCK7

Depositor

Nenad Bursac

Article

Madden et al eLife. 2015 Jan 9;4:e04885. doi: 10.7554

Insert

GCaMP6

Use

Lentiviral

Tags

6xHis, T7 tag, and Xpress epitope

Expression

Mammalian

Promoter

MHCK7

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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pTag-BFP-C-h-Rab7L1-c-Myc

Plasmid

#79803

Purpose

Express TagBFP labeled human Rab7L1.

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

Rab7L1 (RAB29 Human)

Tags

TagBFP and myc

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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