We narrowed to 5,620 results for: pcas

1. pSpCas9(BB)-2A-Puro-V2.0-sg2HsTNRC6A_AH

   Plasmid

   #148852

   Purpose

   Mammalian Expression of HsTNRC6A-sgRNA2

   Depositor

   Elisa Izaurralde

   Insert

   HsTNRC6A-sgRNA2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSpCas9(BB)-2A-Puro-V2.0-sg3HsTNRC6B_AH

   Plasmid

   #148853

   Purpose

   Mammalian Expression of HsTNRC6B-sgRNA3

   Depositor

   Elisa Izaurralde

   Insert

   HsTNRC6B-sgRNA3
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpCas9(BB)-2A-Puro-V2.0-sgHsAGO1_AH

   Plasmid

   #148854

   Purpose

   Mammalian Expression of HsAGO1-sgRNA

   Depositor

   Elisa Izaurralde

   Insert

   HsAGO1-sgRNA (AGO1 S.pyogenes)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSpCas9(BB)-2A-Puro-V2.0-sgHsNot1_AH

   Plasmid

   #148856

   Purpose

   Mammalian Expression of HsNot1-sgRNA

   Depositor

   Elisa Izaurralde

   Insert

   HsNot1-sgRNA (CNOT1 S.pyogenes)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpCas9(BB)-2A-Puro-V2.0-sgHsPatL_AH

   Plasmid

   #148857

   Purpose

   Mammalian Expression of HsPatL1-sgRNA

   Depositor

   Elisa Izaurralde

   Insert

   HsPatL1-sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpCas9(BB)-2A-Puro-V2.0-sgHsTNRC6C_AH

   Plasmid

   #148858

   Purpose

   Mammalian Expression of HsTNRc6C-sgRNA

   Depositor

   Elisa Izaurralde

   Insert

   HsTNRc6C-sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAS-mak10 gRNA-MAK10Δ

   Plasmid

   #160384

   Purpose

   For CRISPR-mediated deletin og MAK10 in yeast

   Depositor

   John McCusker

   Insert

   MAK10
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PGK1

   Available since

   Feb. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pXD70LacZ8-CadR-Pcadh-LacZ

   Plasmid

   #191623

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (KanR), Pcadh catechin dehydroxylase promoter-lacZ with transcriptional regulator CadR

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   lacZ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-Tesmin-BioID2-3xFLAG

   Plasmid

   #186815

   Purpose

   CAG promoter-driven expression of TESMIN-BioID2-3xFLAG

   Depositor

   Masahito Ikawa

   Article

   Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

   Insert

   Tesmin (Tesmin Mouse)
   

   Use

   Tags

   BioID2-3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-MBP-ATG9-Q835A

    Plasmid

    #188081

    Purpose

    Expresses human ATG9-Q835A mutant in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG9 (ATG9A Human)
    

    Use

    Tags

    MBP tag

    Expression

    Mammalian

    Mutation

    Q835A

    Promoter

    CMV

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-MBP-ATG9-K838A

    Plasmid

    #188078

    Purpose

    Expresses human ATG9-K838A mutant in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG9 (ATG9A Human)
    

    Use

    Tags

    MBP tag

    Expression

    Mammalian

    Mutation

    K838A

    Promoter

    CMV

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-MBP-ATG9-V839A

    Plasmid

    #188077

    Purpose

    Expresses human ATG9-V839A mutant in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG9 (ATG9A Human)
    

    Use

    Tags

    MBP tag

    Expression

    Mammalian

    Mutation

    V839A

    Promoter

    CMV

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCALNL-tdTomato-2A-mSyp::EGFP

    Plasmid

    #191213

    Purpose

    Expresses tdTomato protein and mouse Synaptophysin protein fused with EGFP in Cre expressing mammalian cells.

    Depositor

    Theofanis Karayannis

    Article

    Gesuita et al STAR Protoc. 2022 Nov 11;3(4):101837. do

    Insert

    tdTomato-T2A-mSyp::EGFP (Syp Mouse)
    

    Use

    Pcagen

    Tags

    mSyp::EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-WIPI2d (FRRG/AAAA)-TS

    Plasmid

    #190037

    Purpose

    Expression of wipi2d mutant

    Depositor

    James Hurley

    Article

    Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Insert

    WIPI2d (WIPI2 Human)
    

    Use

    Tags

    TEV-2xStrep tag

    Expression

    Mammalian

    Mutation

    223FRRG to AAAA

    Promoter

    CMV

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. 2xNLS-NLP-cMyc AspCas12a

    Plasmid

    #182120

    Purpose

    pET21a protein expression vector for 2xNLS-NLP-cMyc AspCas12a in bacteria

    Depositor

    Scot Wolfe

    Article

    Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

    Insert

    2xNLS-NLP-cMyc AspCas12a
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-MBP-ATG9-L832A

    Plasmid

    #188084

    Purpose

    Expresses human ATG9-L832A mutant in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG9 (ATG9A Human)
    

    Use

    Tags

    MBP tag

    Expression

    Mammalian

    Mutation

    L832A

    Promoter

    CMV

    Available since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-MBP-ATG9-V836A

    Plasmid

    #188080

    Purpose

    Expresses human ATG9-V836A mutant in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG9 (ATG9A Human)
    

    Use

    Tags

    MBP tag

    Expression

    Mammalian

    Mutation

    V836A

    Promoter

    CMV

    Available since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-MBP-ATG9-H837A

    Plasmid

    #188079

    Purpose

    Expresses human ATG9-H837A mutant in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG9 (ATG9A Human)
    

    Use

    Tags

    MBP tag

    Expression

    Mammalian

    Mutation

    H837A

    Promoter

    CMV

    Available since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-MBP-ATG9 (1-830)

    Plasmid

    #188086

    Purpose

    Expresses human ATG9 C-terminal truncation mutant in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG9 (ATG9A Human)
    

    Use

    Tags

    MBP tag

    Expression

    Mammalian

    Mutation

    ATG9 (1-830)

    Promoter

    CMV

    Available since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-MBP-ATG9-P833A

    Plasmid

    #188083

    Purpose

    Expresses human ATG9-P833A mutant in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG9 (ATG9A Human)
    

    Use

    Tags

    MBP tag

    Expression

    Mammalian

    Mutation

    P833A

    Promoter

    CMV

    Available since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCAG-GST-ATG101-Y45D

    Plasmid

    #188105

    Purpose

    Expresses human ATG101 HORMA Y45D mutant in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG101 (ATG101 Human)
    

    Use

    Tags

    GST tag

    Expression

    Mammalian

    Mutation

    codon-optimized ATG101-Y45D

    Promoter

    CMV

    Available since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCAG-OSF-ATG13 (2-197)

    Plasmid

    #188075

    Purpose

    To make ATG101/ATG13 HORMA complex for expression in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG13 (ATG13 Human)
    

    Use

    Tags

    strep-strep-Flag tag

    Expression

    Mammalian

    Mutation

    ATG13 (2-197)

    Promoter

    CMV

    Available since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCAGGS-eDHFR(69K6)-iRFP713

    Plasmid

    #178855

    Purpose

    Mammalian expression of eDHFR(69K6)-iRFP713

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    eDHFR(69K6)-iRFP713
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCasper-pNos-MCP-mCherry

    Plasmid

    #180275

    Purpose

    to epress MCP-mCherry fusion protein in the early fly embryos (driven by maternal nanos driver)

    Depositor

    Hernan Garcia

    Article

    Liu et al PLoS Comput Biol. 2021 May 18;17(5):e100

    Insert

    MCP-mCherry
    

    Use

    Tags

    mCherry

    Expression

    Insect

    Mutation

    Promoter

    nanos

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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25. pJCC_164 SpCas9 R1333A/R1335A

    Plasmid

    #179528

    Purpose

    For bacterial expression of SpCas9 R1333A/R1335A (PAM-binding arginines mutant) with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

    Insert

    SpCas9 R1333A/R1335A
    

    Use

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    changed arginines 1333 and 1335 to alanine

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCAG1.1-Armc12 delta N/FLAG

    Plasmid

    #180495

    Purpose

    Expression vector of N-terminal deleted mouse Armc12 tagged with FLAG at C-terminus. See Fig.4D of Shimada, K. et al. Proc. Natl. Acad. Sci. USA. 118 (6): e2018355118, 2021.

    Depositor

    Masahito Ikawa

    Article

    Shimada et al Proc Natl Acad Sci U S A. 2021 Feb 9;118

    Insert

    armadillo repeat containing 12 (Armc12 Mouse)
    

    Use

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    N-terminus of Armc12 is deleted

    Promoter

    CAG promoter

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCAG1.1-Armc12 delta C/FLAG

    Plasmid

    #180497

    Purpose

    Expression vector of C-terminal deleted mouse Armc12 tagged with FLAG at C-terminus. See Fig.4D of Shimada, K. et al. Proc. Natl. Acad. Sci. USA. 118 (6): e2018355118, 2021.

    Depositor

    Masahito Ikawa

    Article

    Shimada et al Proc Natl Acad Sci U S A. 2021 Feb 9;118

    Insert

    armadillo repeat containing 12 (Armc12 Mouse)
    

    Use

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    C-terminus of Armc12 is deleted

    Promoter

    CAG promoter

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCAG-hSPATA33-8xHIS-1D4

    Plasmid

    #179133

    Purpose

    Expression vector of human spermatogenesis associated 33 (SPATA33) tagged with 8XHIS and 1D4 at C-terminus, CAG promoter, rabbit globin poly(A) signal.

    Depositor

    Masahito Ikawa

    Article

    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

    Insert

    spermatogenesis associated 33 (SPATA33 Human)
    

    Use

    Tags

    1D4 and 8XHIS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCAG-WIPI2dR108E-cs-TEV-STREP

    Plasmid

    #171835

    Purpose

    Mammalian expression of human WIPI2d R108E with C-terminal Strep

    Depositor

    James Hurley

    Article

    Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

    Insert

    WIPI2d (WIPI2 Human)
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    R108E

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCAG-WIPI2dR125E-cs-TEV-STREP

    Plasmid

    #171836

    Purpose

    Mammalian expression of human WIPI2d R125E with C-terminal Strep

    Depositor

    James Hurley

    Article

    Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

    Insert

    WIPI2d (WIPI2 Human)
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    R125E

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge