We narrowed to 5,097 results for: puc
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Plasmid#112359PurposeCRISPR donor plasmid to tag human transcription factor MAZ with GFPDepositorInsertMAZ homology arms flanking EGFP-IRES-Neo cassette (MAZ Human)
UseCRISPRMutationhg38:16:29809765:G>AAvailable SinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
LI A-B EF1 c2
Plasmid#54435DepositorInsertpromoter region of A. thaliana EF1alpha1 /AT1G07940.1
UseCloning vectorAvailable SinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAP1698-4
Plasmid#105242Purposemammalian eGFPDepositorInserteGFP
ExpressionBacterialAvailable SinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
AP588-1
Plasmid#71278PurposeExpresses myc::TEV::mTagGFP::TEV::3Xflag in bacteriaDepositorInserttagGFP
TagsTEV::3xFlag and myc::TEVExpressionBacterialAvailable SinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIF251
Plasmid#133935PurposeKanamycin selection marker flanked by ~200 bp of homology to bacteriophage lambda. The selection marker will recombineer 21.3-22.8 kb away from the cosL DNA end of the phage genome.DepositorInsertKanamycin selection marker flanked by ~200 bp of homology to bacteriophage lambda
UseΛ-phage recombineering plasmidAvailable SinceMarch 3, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCcmO-StrepII-PDT
Plasmid#165584PurposeNative CcmO fusion with a StrepII tag and PDTDepositorInsertCcmO-PDT
TagsProtein degradation tagExpressionBacterialAvailable SinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVC-CRISPR1
Plasmid#120366PurposePlasmid containing shortened version of upstream V-C CRISPR array for integration assaysDepositorInsertCRISPR1
UseCRISPRAvailable SinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVC-CRISPR
Plasmid#120369PurposePlasmid containing shortened versions of both natural V-C CRISPR arrays for preparing sequencing libraryDepositorInsertsCRISPR1
CRISPR2
UseCRISPRAvailable SinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVD_CRISPR
Plasmid#120370PurposePlasmid containing shortened version of V-D CRISPR array for integration assaysDepositorInsertVD_CRISPR
UseCRISPRAvailable SinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
S13_EcoRV_UBC_AscI
Plasmid#128477PurposeUBC promoterDepositorInsertUBC promoter
ExpressionBacterialAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHWBF01
Plasmid#131022PurposeSynthetic source clone for maize Histon H2B.5 with C-terminal mCherry fusionDepositorInsertHistone H2B.5
UseCloning vectorTagsmCherryAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHEY1-donor
Plasmid#112297PurposeCRISPR donor plasmid to tag human transcription factor HEY1 with GFPDepositorInsertHEY1 homology arms flanking EGFP-IRES-Neo cassette (HEY1 Human)
UseCRISPRMutationhg38:8:79765311:G>AAvailable SinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGGF008
Plasmid#48848PurposeProvides a BASTA resistance cassette as GreenGate module.DepositorInsertpNOS::BastaR::tNOS
UseGolden gate compatible cloning vectorMutationchi sequence in BastaR removed by silent substitu…Available SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
S6_BstBI_SV40_SwaI
Plasmid#128470PurposeSV40 promoterDepositorInsertSV40 promoter
ExpressionBacterialAvailable SinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAS_gpyrG1
Plasmid#90276Purpose(also pMST621-BB3_gpyrG1_cas9) CRISPR/Cas9 plasmid with gRNA for pyrG1, Cas9DepositorInsertgRNA (pyrg1)
UseA. nigerExpressionBacterialAvailable SinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGJJ055
Plasmid#183622PurposeddPCA mutagenesis plasmid - Efficient mutant library cloning plasmid suitable for oligo synthesis, error prone PCR or nicking mutagenesis strategies.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAP635-2
Plasmid#99485PurposeMyc::TEV::3XFlag::Dendra2::3XFlag::TEV::MycDepositorInsertMyc::TEV::3XFlag::Dendra2::3XFlag::TEV::Myc
ExpressionBacterialAvailable SinceSept. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4I
Plasmid#104504PurposeUsed to evaluate the expression output of Saccharomyces kudriavzevii GAL1 promoter with yEGFP used as the reporter geneDepositorInsertSkGAL1 promoter-yEGFP
ExpressionYeastAvailable SinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4Q
Plasmid#104512PurposeUsed to evaluate the expression output of Saccharomyces kudriavzevii GAL2 promoter with yEGFP used as the reporter geneDepositorInsertSkGAL2 promoter-yEGFP
ExpressionYeastAvailable SinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4L
Plasmid#104507PurposeUsed to evaluate the expression output of Saccharomyces mikatae GAL2 promoter with yEGFP used as the reporter geneDepositorInsertSmGAL2 promoter-yEGFP
ExpressionYeastAvailable SinceNov. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4H
Plasmid#104503PurposeUsed to evaluate the expression output of Saccharomyces arboricola GAL1 promoter with yEGFP used as the reporter geneDepositorInsertSaGAL1 promoter-yEGFP
ExpressionYeastAvailable SinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4J
Plasmid#104505PurposeUsed to evaluate the expression output of Saccharomyces mikatae GAL1 promoter with yEGFP used as the reporter geneDepositorInsertSmGAL1 promoter-yEGFP
ExpressionYeastAvailable SinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4N
Plasmid#104509PurposeUsed to evaluate the expression output of Saccharomyces bayanus GAL2 promoter with yEGFP used as the reporter geneDepositorInsertSbGAL2 promoter-yEGFP
ExpressionYeastAvailable SinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4R
Plasmid#104513PurposeUsed to evaluate the expression output of Saccharomyces uvarum GAL2 promoter with yEGFP used as the reporter geneDepositorInsertSuGAL2 promoter-yEGFP
ExpressionYeastAvailable SinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDLX1-donor
Plasmid#112343PurposeCRISPR donor plasmid to tag human transcription factor DLX1 with GFPDepositorInsertDLX1 homology arms flanking EGFP-IRES-Neo cassette (DLX1 Human)
UseCRISPRMutationhg38:2:172087403:G>C,hg38:2:172087433:G>A,h…Available SinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJAID6A
Plasmid#165046PurposeIntegrative expression of yEGFP and rice auxin receptor gene OsTIR1. OsTIR1 is codon-optimised for expression in S. cerevisiaeDepositorInsertP-URA3>KlURA3>TAgTEF1-P-TEF1>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
ExpressionYeastMutationWTAvailable SinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
BC1364-mouse U6-sgH2B
Plasmid#164043PurposeU6-driven sgRNA targeting H2BDepositorInsertH2B targeting sgRNA
UseCRISPRPromotermouse U6Available SinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pML1
Plasmid#108332PurposeMIDAS Level-1 source vector for cloning of modules (synthetic biology parts)DepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
S7_SwaI_Zeo_PacI
Plasmid#128471PurposeZeo Resistance geneDepositorInsertZeocin Resistance gene
ExpressionBacterialAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
S12_EcoRV_PGK_AscI
Plasmid#128476PurposePGK promoterDepositorInsertPGK promoter
ExpressionBacterialAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDDIT3-donor
Plasmid#112300PurposeCRISPR donor plasmid to tag human transcription factor DDIT3 with GFPDepositorInsertDDIT3 homology arms flanking EGFP-IRES-Neo cassette (DDIT3 Human)
UseCRISPRAvailable SinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJAID8Z104
Plasmid#165056PurposeIntegrative expression of yEGFP-AID*-CUP1 and SKP1-fusing rice auxin receptor gene OsTIR1.DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>T-PGK1-P-ACS2> SKP1>OsTIR1opt>T-URA3
ExpressionYeastMutationWTAvailable SinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
AP625-1
Plasmid#70051PurposeExpresses myc::TEV::3Xflag::eGFP::3Xflag::TEV::myc in bacteriaDepositorInserteGFP
Tags3Xflag::TEV::myc and myc::TEV::3XflagExpressionBacterialAvailable SinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHOXB5-donor
Plasmid#113815PurposeCRISPR donor plasmid to tag human transcription factor HOXB5 with GFPDepositorInsertHOXB5 homology arms flanking EGFP-IRES-Neo cassette (HOXB5 Human)
UseCRISPRMutationrs2288276, rs7406798, rs36049616, rs3087996Available SinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDiySpike2
Plasmid#136949PurposePlasmid for the In Vitro transcription of DiySpike2DepositorInsertDiySpike2
UseSynthetic BiologyPromoterT7Available SinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-NEO-CAG-BFP-pA-GFP-pA
Plasmid#149344PurposeVector for targeting the Replace reporter to the AAVS1 locusDepositorInsertBFP
ExpressionMammalianPromoterCAGAvailable SinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
B3rec.zf1
Plasmid#108354PurposeZebrafish codon optimized B3 recombinase that acts on blown-out sitesDepositorInsertB3 recombinase
UseSynthetic BiologyMutationZebrafish codon optimizedPromoterlacAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMST665_BB3_L 23_gRNAempty_cas9_BbsI
Plasmid#90278PurposeBB3_L 23_gRNAempty_cas9_BbsI; CRISPR plasmid with empty gRNA spacer to incorporate gRNA, Cas9DepositorInsertCas9
UseA. nigerExpressionBacterialAvailable SinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAP639-2F
Plasmid#99486Purpose3XFlag::TEV::6XHis::mTurquoise::6XHis::TEV::3XFlagDepositorInsert3XFlag::TEV::6XHis::mTurquoise::6XHis::TEV::3XFlag
ExpressionBacterialAvailable SinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
LI B-C N-NLS KOZ c2
Plasmid#54443DepositorInsertNuclear localization signal (NLS) of SV40 large T antigen
UseCloning vectorAvailable SinceNov. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGGD007
Plasmid#48837PurposeProvides linker:nuclear localization signal as GreenGate C-terminal tag module.DepositorInsertlinker:nuclear localization signal
UseGolden gate compatible cloning vectorAvailable SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCLOCK-donor
Plasmid#112310PurposeCRISPR donor plasmid to tag human transcription factor CLOCK with GFPDepositorInsertCLOCK homology arms flanking EGFP-IRES-Neo cassette (CLOCK Human)
UseCRISPRMutationhg38:4:55435551:A>G, hg38:4:55435749:G>AAvailable SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAP608-1
Plasmid#99484PurposeH2B::linker::GFP (contain 3 introns)::3XFlag::TEV::Myc::tbb-2 3UTRDepositorInsertH2B::linker::GFP with introns::3XFlag::TEV::Myc::tbb-2 3UTR
ExpressionBacterialAvailable SinceSept. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAP607-2
Plasmid#99483PurposeMyc::TEV::3XFlag::GFP::3XFlag::TEV::MycDepositorInsertMyc::TEV::3XFlag::GFP::3XFlag::TEV::Myc
ExpressionBacterialAvailable SinceSept. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
LI C-D 1-10GFP noATG
Plasmid#54462DepositorInsertsuperfolder GFP 1-10 domain without start codon
UseCloning vectorAvailable SinceNov. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pML3
Plasmid#108341PurposeMIDAS Level-3 destination vector. Used for initiating Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGRE NPD-GFP
Plasmid#1197DepositorInsertN-terminus of Sup35 fused to GFP (SUP35 Budding Yeast)
TagsGFPExpressionYeastMutationfused N-terminal Sup35 (~1-253aa) to GFPAvailable SinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pJK571
Plasmid#75379PurposeProduces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits and associated hypothetical protein with N-terminal His6 tag (AaPurS, H6AaOrfY, AaPurQ, AaPurL-G209D)DepositorInsertsformylglycinamidine ribonucleotide synthetase, small subunit
hypothetical protein in purSQL operon
formylglycinamidine ribonucleotide synthetase, glutaminase subunit
formylglycinamidine ribonucleotide synthetase, large subunit
UseGene synthesisTagsHis6ExpressionBacterialMutationchanges glycine-209 to aspartate and synthetic ge…Promotern/aAvailable SinceAug. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
CJ41 (U1 snRNA (U1;21d))
Plasmid#17774DepositorInsertU1 snRNA (U1;21d) (snRNA:U1:21D Fly)
Available SinceApril 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
LI B-C N-NES KOZ b c7
Plasmid#54445DepositorInsertNuclear Export Signal (NES) of heat stable inhibitor (PKI)
UseCloning vectorAvailable SinceJune 18, 2014AvailabilityAcademic Institutions and Nonprofits only