We narrowed to 14,061 results for: CAN
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Plasmid#194715PurposeBicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that can be cloned with BbsI sitesDepositorInsertAsCpf1
UseCRISPRExpressionMammalianAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO-MCS
Plasmid#185594PurposeControl lentivector that can be used to express inserts from the PGK promoter and/or shRNA cassettes from U6.DepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
4 gRNA concatemer
Plasmid#84881PurposeEmpty concatmer vector in which 4 gRNAs can be insertedDepositorTypeEmpty backboneUseRetroviralExpressionMammalianAvailable SinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
sgRNA_expression_vector
Plasmid#210212PurposeAn empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.DepositorTypeEmpty backboneExpressionBacterial and MammalianPromoterU6Available SinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
modRNAc1-VP64dCas9VP64-2A-MPH
Plasmid#216388Purposethis vector can be used to make modRNA expressing VP64dCas9VP64 and MPHDepositorInsertVP64dCas9VP64-2A-MPH
UseCRISPRAvailable SinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
modRNAc1-Cas9-2A-p53DD
Plasmid#216389Purposethis vector can be used to make modRNA expressing Cas9 and p53DDDepositorInsertCas9-2A-p53DD
UseCRISPRAvailable SinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
gRNA Cloning Vector Bbs I ver. 2
Plasmid#85586PurposeAn empty sgRNA expression vector. sgRNA sequence can be cloned into its Bbs I site. Its gRNA scaffold has efficient ver. 2 structure.DepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterU6Available SinceDec. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
px459V2.0_Conc2
Plasmid#134451PurposeEmpty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + PuroDepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterU6Available SinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
mini-donor - NKX6.1-nEGFP
Plasmid#206047Purposethis mini-donor knockin vector can be used to knock nuclear EGFP into NKX6.1 locusDepositorInsertnuclear EGFP
Available SinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
px459V2.0_Conc3
Plasmid#134458PurposeEmpty concatemer vector in which 3 sgRNAs can be inserted; with Cas9 + PuroDepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterU6Available SinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHA2
Plasmid#85710PurposeThis plasmid provides a BAC vector backbone containing GFP, which can be used for cloning of BAC inserts, for instance, herpesvirus genomesDepositorTypeEmpty backboneUseCre/Lox; Herpesvirus cloningExpressionBacterialAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmtb-t7-alpha-bungarotoxin
Plasmid#69542PurposeFor in vitro transcription of alpha-bungarotoxin mRNA that can be injected into embryos.DepositorInsertalpha-bungarotoxin
UseTol2, beta-actin promoter, sp6,t7 for zebrafishPromoterbeta-actin, sp6, t7Available SinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRGEB32-BAR
Plasmid#126072PurposeFor CRISPR/Cas9 delivery in maize. Expresses Cas9 with rice ubiquitin promoter, BAR with 35S promoter, and sgRNA/PTG with rice snoRNA U3 promoter (pol III). Can produce 1 or more gRNAs.DepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoter35S for BAR; Rice UBI for Cas9; Rice snoRNA U3 fo…Available SinceJune 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
Rab19 cds in pGem-T Easy
Plasmid#80504PurposeCan be used for in situ hybridisation or other purposesDepositorInsertrab19 (rab19 Zebrafish)
Available SinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPSup_iGFP
Plasmid#149420PurposePlasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted just upstream of the minimal promoter. The GFP reporter gene harbors the IV2 intron.DepositorTypeEmpty backboneExpressionPlantAvailable SinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pC1-HyPer-C199S
Plasmid#42213PurposePeroxide-insensitive version of HyPer to be used as a pH-control. Can be applied as a pH sensor.DepositorInsertHYPER C199S
ExpressionMammalianPromoterCMVAvailable SinceDec. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pACλCI-β-flap (831-1057)
Plasmid#53733PurposeExpression of λCI fused via three alanines to E. coli RNAP beta subunit, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.DepositorInsertλCI-β-flap fusion
ExpressionBacterialPromoterlac UV5Available SinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBRα-β flap (831-1057)
Plasmid#53734PurposeExpression of alpha NTD fused via three alanines to E. coli RNAP subunit beta, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.DepositorInsertalpha NTD-Beta flap fusion
ExpressionBacterialPromoterlpp/lacUV5 (tandem promoter)Available SinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDonor_RB-TnV2
Plasmid#213907PurposeBarcoded mariner transposon with kan selection casette, and modified terminal sequences - barcodes can be added via FseI-SbfI restriction sitesDepositorInsertmariner transposon with kan resistance and FseI-SbfI sites for barcode insertion
ExpressionBacterialAvailable SinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCFD3-NS
Plasmid#149545PurposeExpression of single pegRNA under control of Drosophila U6:3 promoter. NS stands for "No Scaffold". Can be used to generate transgenic flies with vermillion+ selection.DepositorTypeEmpty backboneUseCRISPRExpressionInsectPromoterU6Available SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only