We narrowed to 18,294 results for: MUT

1. CMVTO-nTVMVp-AP4-tevs-P3-tevs-cTVMVPmut-CAAX

   Plasmid

   #179662

   Purpose

   Membrane-bound (CAAX) TEVP-inducible caged-nTVMVP

   Depositor

   Xiaojing Gao

   Article

   Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

   Insert

   Caged-nTVMVP
   

   Expression

   Mammalian

   Available Since

   March 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-CMV-EGFP-PolB(K72A)-PAMmut-Hygro

   Plasmid

   #176087

   Purpose

   EGFP fused to the N-terminus of POLB containing the mutation Lys72Ala, a mutation in the PAM site used by POLBKOg1 & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   PolB (POLB Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Mutation in Lys72 to Ala, and mutation in the PAM…

   Promoter

   CMV

   Available Since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSP72-RBM8A-3'UTR AluY-Sz mut 2598-4066

   Plasmid

   #175586

   Purpose

   in vitro transcription of mut RBM8A 3'UTR

   Depositor

   Emanuela Felley-Bosco

   Article

   Abukar et al Cells. 2021 Dec 15;10(12). pii: cells101

   Insert

   RBM8A-3'UTR AluY-Sz mut 2598-4066
   

   Use

   In vitro transcription

   Promoter

   T7

   Available Since

   Dec. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330-Flag-evoSpCas9 (without sgRNA; with silent mutations)

   Plasmid

   #126758

   Purpose

   Expression plasmid for human codon-optimized increased fidelity evoSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   evoSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   M495V, Y515N, K526E, R661Q

   Promoter

   CBh

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330-Flag-HeFSpCas9 (without sgRNA; with silent mutations)

   Plasmid

   #126759

   Purpose

   Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   HeFSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

   Promoter

   CBh

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330-Flag-WT_SpCas9 (without sgRNA; with silent mutations)

   Plasmid

   #126753

   Purpose

   Expression plasmid for human codon-optimized WT SpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   WT SpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330-Flag-eSpCas9 (without sgRNA; with silent mutations)

   Plasmid

   #126754

   Purpose

   Expression plasmid for human codon-optimized increased fidelity eSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   eSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   K848A, K1003A, R1060A

   Promoter

   CBh

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330-Flag-HypaSpCas9 (without sgRNA; with silent mutations)

   Plasmid

   #126756

   Purpose

   Expression plasmid for human codon-optimized increased fidelity HypaSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   HypaSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N692A, M694A, Q695A, H698A

   Promoter

   CBh

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFPC1/GgVcl 1-1066 A50I K1306A, R1308A mutant

   Plasmid

   #46267

   Depositor

   Susan Craig

   Insert

   Vcl (VCL Chicken)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   K1306A, R1308A, A50I mutation; talin binding defi…

   Promoter

   CMV

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTYB3-human PUM1-HD MUT7-1 (E1083Q)-intein-CBD

    Plasmid

    #73303

    Purpose

    Expresses mutant human PUM1-HD

    Depositor

    Traci Hall

    Article

    Cheong et al Proc Natl Acad Sci U S A. 2006 Sep 12;10

    Insert

    human PUM1 Pumilio homology domain (PUM1 Human)
    

    Tags

    intein

    Expression

    Bacterial

    Mutation

    E1083Q

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pMIR-Report-SPRED1-3'UTR (mut - miR-21 site1)

    Plasmid

    #62580

    Purpose

    Translational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The upstream miR-21 (site 1) binding site was mutated.

    Depositor

    Michael Ruppert

    Article

    Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

    Insert

    SPRED1 (SPRED1 Human)
    

    Use

    Luciferase

    Mutation

    miR-21 binding site 1 mutant (TAAGCTA --> TAGA…

    Available Since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pMIR-Report-SPRED1-3'UTR (mut - miR-21 site 2)

    Plasmid

    #62581

    Purpose

    Translational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The downstream miR-21 binding site (site 2) was mutated.

    Depositor

    Michael Ruppert

    Article

    Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

    Insert

    SPRED1 (SPRED1 Human)
    

    Use

    Luciferase

    Mutation

    miR-21 binding site 2 mutant (TAAGCTA --> TAGA…

    Available Since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

13. VV017: Archaerhodopsin-3 w/ D95Q point mutation fused to eGFP

    Plasmid

    #58490

    Purpose

    Expresses Arch(D95Q)-eGFP in mammalian cells as a fluorescent reporter of membrane voltage

    Depositor

    Adam Cohen

    Article

    Venkatachalam et al J Am Chem Soc. 2014 Feb 12;136(6):2529-3

    Insert

    Archaerhodopsin-3 w/ D95H point mutation fused to eGFP
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Changed Aspartic Acid 95 to Glutamine

    Promoter

    Ubiquitin

    Available Since

    Aug. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-GST-TNRC6A-(935-984)-NES-mut

    Plasmid

    #42051

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Tags

    GST and His

    Expression

    Bacterial

    Mutation

    contain aa935-984, F951A, F955A, I958A, F960A

    Available Since

    April 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmyc-GFP-TNRC6A-del.GW-II+III-NES-mut

    Plasmid

    #42043

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    deleted aa716-772 and aa804-924, F951A, F955A, I9…

    Promoter

    CMV

    Available Since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pmyc-GFP-TNRC6A-del.GW-I+III-NES-mut

    Plasmid

    #42042

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    deleted aa457-532 and aa804-924, F951A, F955A, I9…

    Promoter

    CMV

    Available Since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pmyc-GFP-TNRC6A-del.GW-III-NES-mut

    Plasmid

    #42041

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    deleted aa804-924, F951A, F955A, I958A, F960A

    Promoter

    CMV

    Available Since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmyc-GFP-TNRC6B-(997-1084)-NES-mut

    Plasmid

    #42025

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6B (TNRC6B Human)
    

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    contain aa997-1084, L1009A, F1013A, M1016A, L1018A

    Promoter

    CMV

    Available Since

    March 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. CJ38 (miR-1006 seed mutant perfect site)

    Plasmid

    #17771

    Depositor

    David Bartel

    Article

    Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

    Insert

    miR-1006 seed mutant perfect site
    

    Use

    Luciferase; Drosophila metallothionein gene promo…

    Tags

    renilla luciferase

    Expression

    Insect

    Mutation

    seed mutant

    Available Since

    April 15, 2008

    Availability

    Academic Institutions and Nonprofits only

20. CJ39 (miR-1003 seed mutant perfect site)

    Plasmid

    #17772

    Depositor

    David Bartel

    Article

    Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

    Insert

    miR-1003 seed mutant perfect site
    

    Use

    Luciferase; Drosophila metallothionein gene promo…

    Tags

    renilla luciferase

    Expression

    Insect

    Mutation

    seed mutant

    Available Since

    April 15, 2008

    Availability

    Academic Institutions and Nonprofits only