We narrowed to 2,598 results for: ubiquitin

1. pcDNA5FRT/TO-UBE2QL1-GFP

   Plasmid

   #124667

   Purpose

   Expresses UBE2QL1 with C-terminal GFP tag in mammalian cells

   Depositor

   Hemmo Meyer

   Article

   Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

   Insert

   UBE2QL1 (UBE2QL1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEGFP-WRAP53beta-WD40domain

   Plasmid

   #64678

   Purpose

   Expresses the WD40 domain of WRAP53beta

   Depositor

   Marianne Farnebo

   Article

   Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

   Insert

   Wd40-encoding RNA antisense to P53 (WRAP53 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   containing only WD40 domain of WRAP53beta

   Promoter

   CMV

   Available since

   May 21, 2015

   Availability

   Academic Institutions and Nonprofits only

3. XE256 pCDNA3.1(zeo)-hDsh3-deltaCTER

   Plasmid

   #16753

   Depositor

   Randall Moon

   Article

   Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

   Insert

   FLAG-hDsh3-deltaCTER (DVL3 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   delta CTER

   Promoter

   Available since

   March 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEGFPc3 N-RAS

   Plasmid

   #236450

   Purpose

   transient overexpression of N-RAS in mammalian cells

   Depositor

   Michele Pagano

   Article

   Kuchay et al Nat Struct Mol Biol. 2019 Jul;26(7):628-

   Insert

   N-RAS (NRAS Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAM316 pET28a-GGG-Titin[V15P]-23-K-9-C-14cyclinB-10GS-Y

   Plasmid

   #226349

   Purpose

   Expression of Titin V15P substrate with GS tail

   Depositor

   Andreas Martin

   Article

   Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

   Insert

   titin (TTN Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   V15P

   Promoter

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAM356 pET28a-GGG-Titin[V15P]-23-K-29-C-5

   Plasmid

   #226352

   Purpose

   Expression of Titin (V15P) substrate for 26S proteasome

   Depositor

   Andreas Martin

   Article

   Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

   Insert

   titin (TTN Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   V15P

   Promoter

   T7

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAM357 pET28a-GGG-Titin[V15P]-23-K-33-C-1

   Plasmid

   #226353

   Purpose

   Expression of titin (V15P) substrate for 26S proteasome

   Depositor

   Andreas Martin

   Article

   Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

   Insert

   titin (TTN Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   V15P

   Promoter

   T7

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGFP-mVprBP pc2953

   Plasmid

   #201698

   Purpose

   Expresses of GFP-tagged VprBP in mammalian cells

   Depositor

   Cristina Cardoso

   Article

   Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

   Insert

   VprBP (Dcaf1 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pmcherry-mVprBP pc2954

   Plasmid

   #201699

   Purpose

   Expresses of mcherry-tagged mVprBP in mammalian cells

   Depositor

   Cristina Cardoso

   Article

   Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

   Insert

   VprBP (Dcaf1 Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pmcherry-mTet1s pc3905

    Plasmid

    #201703

    Purpose

    Expresses of mcherry-tagged mTet1s in mammalian cells

    Depositor

    Cristina Cardoso

    Article

    Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

    Insert

    Tet1s (Tet1 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGAD424-Rabex-5

    Plasmid

    #197043

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-Rabex-5 fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2006 Mar 10;281(10):6874-83

    Insert

    Rabex-5 (RABGEF1 Bovine)
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET-Duet1-6xHis-TEV-OPTN

    Plasmid

    #190192

    Purpose

    Plasmid for the expression of 6xHis-TEV-OPTN. Internal reference: SMC397

    Depositor

    Sascha Martens

    Article

    Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

    Insert

    optineurin (OPTN Human)
    

    Use

    Tags

    6xHis and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRSFDuet1-His-TEV-OTC_d1_Ub

    Plasmid

    #170729

    Purpose

    Scaffold design for studying ubiquitin interacting proteins by cryo-EM.

    Depositor

    Shang-Te Danny Hsu

    Article

    Chiu et al Biochemistry. 2021 Apr 13;60(14):1075-10

    Insert

    E. coli ornithine transcarbamylase (OTC), fused to human ubiquitin
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    The C-terminus of OTC was truncated by removing o…

    Promoter

    T7

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDONR221 Ub-NLRP1 L1215

    Plasmid

    #166828

    Purpose

    pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate a L1215 N-terminus (no stop)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Ubiquitin

    Expression

    Mutation

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDONR221 Ub-NLRP1 S1213

    Plasmid

    #166827

    Purpose

    pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate the native S1213 N-terminus (no stop)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Ubiquitin

    Expression

    Mutation

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDONR221 Ub-NLRP1 L1218

    Plasmid

    #166829

    Purpose

    pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate a L1218 N-terminus (no stop)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Ubiquitin

    Expression

    Mutation

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX6p1 AO7R

    Plasmid

    #137842

    Purpose

    GST tagged AO7 encoding aa 126-216 for expression in bacteria

    Depositor

    Allan Weissman

    Article

    Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

    Insert

    AO7 (RNF25 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. FLAG-HA-RNF68 [Y252D]

    Plasmid

    #126550

    Purpose

    Expresses FLAG-HA-tagged RNF68 [Y252D] mutant in mammalian cells

    Depositor

    Michele Pagano

    Article

    Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

    Insert

    RNF68 (PCGF1 Human)
    

    Use

    Retroviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    Y252D point mutant

    Promoter

    LTR

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLVX-BirA-UBE3B-Puro

    Plasmid

    #107351

    Purpose

    Expresses UBE3B fused to BirA in mammalian cells.

    Depositor

    Robert Sobol

    Article

    Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

    Insert

    BirA-UBE3B (UBE3B Human)
    

    Use

    Lentiviral

    Tags

    Biotin ligase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 7, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pCMV-FLAG-WRAP53beta-siRNA-resistant

    Plasmid

    #64680

    Purpose

    expresses siRNA resistant WRAP53beta

    Depositor

    Marianne Farnebo

    Article

    Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

    Insert

    WD40-encoding RNA antisense to P53 (WRAP53 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    siRNA resistant

    Promoter

    CMV

    Available since

    May 22, 2015

    Availability

    Academic Institutions and Nonprofits only