We narrowed to 78,677 results for: TRI
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Plasmid#100033PurposeThis plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 390 nm.DepositorInsertRhoGC E254D
UseTags1D4 (TETSQVAPA)ExpressionMammalianMutationE254DPromoterAdenovirus major late promoterAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTE5418_pMBP-GlcR
Plasmid#221192PurposeExpression vector for 10x-MBP-GlcR (MGlcR). GlcR is a glycolate-responsive transcriptional repressor and the gene product of pden4400 from Paracoccus denitrificans, codon optimized for E. coli.DepositorInsertglcR (pden4400 from Paracoccus denitrificans)
UseTags10x His tag with E. coli maltose binding protein …ExpressionBacterialMutationPromoterT7 promoter and T7 promoter-lacOAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCas12b
Plasmid#186449PurposeEncodes Cas12b from A. acidoterrestris (type V-B)DepositorInsertCas12b
UseCRISPRTagsExpressionMutationPromoterT7Available sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas12b(PI)
Plasmid#186451PurposeEncodes inactive Cas12b containing mutation in the PI domain (R122A, G143P) from A. acidoterrestris (type V-B)DepositorInsertCas12b (PI)
UseCRISPRTagsExpressionMutationR122A, G143PPromoterT7Available sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pICH47811-pAtAct2::Cas9-nls::tSlATPase
Plasmid#160221PurposeCas9-nuclear localization, pACT2 promoter, SlATPase terminator transcriptional unit, inserted at the MoClo toolkit level 1 acceptor position 2 reverseDepositorInsertpAtACT2::Cas9-nls::tSlATPase
UseSynthetic Biology; Moclo toolkit level 1 acceptor…TagsExpressionPlantMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1046-Tier1-PhCMV-4xCox8-mRuby2
Plasmid#169537PurposeTier-1 vector encoding PhCMV-driven mRuby2 localized to the mitochondrial matrix via a four-fold Cox8 localization signal (PhCMV-4xCox8-mRuby2-pA)DepositorInsertPCMV-driven miochondria-localized monomeric red fluorescent protein mRuby2
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HA-PfROM4 (H578A)
Plasmid#154858PurposeExpresses N-terminally HA-tagged PfROM4 variant H578A (codon optimized for mammalian expression) from pcDNA3.1DepositorInsertPfROM4 (PF3D7_0506900 Synthetic)
UseTagstriple HAExpressionMammalianMutationHistidine 578 changed to AlaninePromoterCMVAvailable sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HA-PfROM4 (G470A/G471N)
Plasmid#154859PurposeExpresses N-terminally HA-tagged PfROM4 variant G470A/G471N (codon optimized for mammalian expression) from pcDNA3.1DepositorInsertPfROM4 (PF3D7_0506900 Synthetic)
UseTagstriple HAExpressionMammalianMutationGlycine 470 changed to Alanine and Glycine 471 ch…PromoterCMVAvailable sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HA-PfROM1 (A144G/N145G)
Plasmid#154873PurposeExpresses N-terminally HA-tagged PfROM1 variant A144G/N145G (codon optimized for mammalian expression) from pcDNA3.1DepositorInsertPfROM1 (PF3D7_0506900 Synthetic)
UseTagstriple HAExpressionMammalianMutationAlanine 144 changed to Glycine and Asparagine 145…PromoterCMVAvailable sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGWB12_TtNAM-B1
Plasmid#124573PurposeExpresses full-length TtNAM-B1 with an N-terminal FLAG tag under the 35s promoter.DepositorInsertTaNAM-B1
UseTagsFLAGExpressionPlantMutationPromoter35sAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR8_TtNAM-B1
Plasmid#124575PurposeTtNAM-B1 CDS in the Gateway-compatible pCR8 vector.DepositorInsertTaNAM-B1
UseTopo gateway cloning vectorTagsExpressionMutationPromoterNoneAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR8_TaNAM-A1(1-217)
Plasmid#124576PurposeNAC domain (1-217) of TaNAM-A1 in the Gateway-compatible pCR8 vector.DepositorInsertTaNAM-A1 (1-217)
UseTopo gateway cloning vectorTagsExpressionMutationPromoterNoneAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR8_TtNAM-B1(1-215)
Plasmid#124577PurposeNAC domain (1-215) of TtNAM-B1 in the Gateway-compatible pCR8 vector.DepositorInsertTtNAM-B1 (1-215)
UseTopo gateway cloning vectorTagsExpressionMutationPromoterNoneAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMT3-RhoGC D380E
Plasmid#100035PurposeThis plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 533 nm.DepositorInsertRhoGC D380E
UseTags1D4 (TETSQVAPA)ExpressionMammalianMutationD380EPromoterAdenovirus major late promoterAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMT3-RhoGC D380N
Plasmid#100034PurposeThis plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 506 nm.DepositorInsertRhoGC D380N
UseTags1D4 (TETSQVAPA)ExpressionMammalianMutationD380NPromoterAdenovirus major late promoterAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGS1412
Plasmid#63787PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eCitrine Strep-tag II followed by URA3 selection markerDepositorInserteCitrine-Strep-tag II followed by URA3 selection marker
UseE. coli cloning vectorTagseCitrine-Strep-tag IIExpressionMutationPromoternoneAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
LAMP1-RpHLuorin2
Plasmid#171720PurposeLysosomal expression of ratiometric pHLuorin2DepositorInsertRatiometric pHLuorin2 fused to LAMP1 (LAMP1 Human)
UseTagsratiometric pHluorin2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
BNLS
Plasmid#50836PurposeExpresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 1 (GANLS-DEVD-BNES or RANLS-DEVD-BNES).DepositorInsertddGFP B
UseTagstriplicated NLS sequence DPKKKRKVExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-double floxed-Gad67-WPRE-HGHpA
Plasmid#184632PurposeExpresses Gad67, driven by the Ef1a promoter, in a Cre-dependent fashionDepositorInsertGad67 (Gad1 Mouse)
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn1_nLightG
Plasmid#187179PurposeExpresses green norepinephrine indicator nLightG in neuronal cellsDepositorHas ServiceAAV9InsertnLightG
UseAAVTagsFlag tagExpressionMutationPromoterhuman Synapsin-1Available sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn1_nLightR
Plasmid#187180PurposeExpresses red norepinephrine indicator nLightR in neuronal cellsDepositorHas ServiceAAV9InsertnLightR
UseAAVTagsFlag tagExpressionMutationPromoterhuman Synapsin-1Available sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
PtPuc3_diaCas9_sgRNA
Plasmid#109219PurposeEpisome based vector with CRISPR/Cas9_sgRNA module for genome editing in Phaeodactylum tricornutumDepositorInsertsLHCF2 promoter
diaCas9
LHCF1 terminator
U6 promoter
sgRNA
U6 3' region
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterLHCF1 terminator, LHCF2 promoter, U6 3' regi…Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-dLight1.1
Plasmid#111067PurposeTo generate Adeno-Associated viruses for expression of dLight1.1 under CAG promoterDepositorHas ServiceAAV1 and AAV5InsertdLight1.1
UseAAVTagsFlag tagExpressionMutationPromoterAvailable sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCD5-PR8-TEV-sfGFP-twinStrep
Plasmid#157933PurposeExpresses influenza A virus trimeric hemagglutinin fused to a sfGFPDepositorInsertA/PuertoRico/8/34 hemagglutinin (HA Synthetic)
UseTagssfGFP & TwinStrepExpressionMammalianMutationE190DPromoterCMVAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynapsin1.OxLight1
Plasmid#169792PurposeFluorescent reporter for orexin neuropeptidesDepositorInsertOxLight1
UseAAVTagsExpressionMutationPromoterhuman Synapsin-1Available sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
RANLS
Plasmid#50843PurposeExpresses one ddFP in mammalian cells, the second plasmid for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with plasmid GANES-DEVD-BNLS.DepositorInsertddRFP A
UseTagstriplicated NLS sequence DPKKKRKVExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
GANLS
Plasmid#50852PurposeExpresses one ddFP in mammalian cells, the second plasmid for a red to green colour switch-based fluorescent caspase-3 biosensor, used together with plasmid NESRA-DEVD-BNLS.DepositorInsertddGFP A
UseTagstriplicated NLS sequence DPKKKRKVExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-dLight1.1
Plasmid#111066PurposeTo generate Adeno-Associated viruses for expression of dLight1.1 under synapsin promoterDepositorHas ServiceAAV1InsertdLight1.1
UseAAVTagsFlag tagExpressionMutationPromoterAvailable sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-dLight1.2
Plasmid#111068PurposeTo generate Adeno-Associated viruses for expression of dLight1.2 under synapsin promoterDepositorHas ServiceAAV1 and AAV5InsertdLight1.2
UseAAVTagsFlag tagExpressionMutationPromoterAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV-REX-GECO1
Plasmid#61246PurposeExpresses REX-GECO1 in mammalian cellsDepositorInsertREX-GECO1
UseTagsExpressionMammalianMutationSubstitutions relative to R-GECO1: P60R, V61W, R6…PromoterCMVAvailable sinceMarch 27, 2015AvailabilityAcademic Institutions and Nonprofits only