Addgene

We narrowed to 6,949 results for: ef1a

Showing: 1951 - 1980 of 6949 results

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cEF.tk-GFP

Plasmid

#33308

Depositor

Linzhao Cheng

Article

Pomper et al Cell Res. 2009 Mar;19(3):370-9.

Insert

HSV-TK/GFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 29, 2012

Availability

Academic Institutions and Nonprofits only

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pGEMS-II

Plasmid

#204765

Purpose

GEMS sensor system (pCMV-EGFP-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577)

Depositor

Kate Meyer

Article

Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

EGFP-m6Alinker-DHFR

Use

Tags

Expression

Mutation

mutated all RACs in EGFP to RGC/RCC

Promoter

CMV

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-Cre-MS2_UbC-NLS-tdMCP-GFP (Cre_tdMCP-GFP)

Plasmid

#219537

Purpose

a. Expresses Cre recombinase tagged with 23x repeats of MS2 binding sequence under EF1A promotor and b. NLS-tdMCP_GFP reporter under UbC promotor

Depositor

Jeffrey Gerst

Article

Haimovich et al bioRxiv 2024.11.06.622258

Inserts

Cre recombinase (cre )
NLS-tdMCP-GFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 11, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pYSEAP

Plasmid

#37326

Depositor

John Schiller

Article

Pastrana et al Virology. 2004 Apr 10;321(2):205-16.

Insert

SEAP

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

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LentiGuide-TagBFP-2A-BSD

Plasmid

#167929

Purpose

Lentiviral gRNA expression with TagBFP fluorescence and blasticidin selection marker.

Depositor

Ronald Germain

Article

Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6, EF1a

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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p11L1w

Plasmid

#37330

Depositor

John Schiller

Article

Mossadegh et al Virology. 2004 Aug 15;326(1):57-66.

Insert

HPV11 L1

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 23, 2013

Availability

Academic Institutions and Nonprofits only

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PCT62

Plasmid

#170391

Purpose

expresses three sgRNA cassettes

Depositor

Jonathan Weissman

Article

Quinn et al Science. 2021 Feb 26;371(6532). pii: sci

Insert

PCT62-targetsite

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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PB-TO-hNGN2-BSD-mApple

Plasmid

#182308

Purpose

Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression, bsd selection, mApple

Depositor

Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

Inserts

hNGN2 (NEUROG2 Human)
bsd-T2A-mycNLS-mApple

Use

Tags

T2A-mycNLS-mApple

Expression

Mammalian

Mutation

Promoter

EF1a and TRE3G

Available since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2 Spike-d18

Plasmid

#164583

Purpose

A mammalian expression vector to express codon-optimized SARS-CoV-2 Spike protein, with a C terminal truncation of 18 amino acids to improve pseudoparticle generation.

Depositor

Jason Sheltzer

Insert

SARS-CoV-2 Spike (S SARS-CoV-2)

Use

Tags

Expression

Mammalian

Mutation

Deleted amino acids 1256-1273

Promoter

EF1a

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLV_Stargazin-mTurquoise2-iLID

Plasmid

#161001

Purpose

Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to Stargazin for slow diffusion

Depositor

Sean R Collins

Article

Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

Insert

Stargazin-mTurquoise2-iLID

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-AP.Baz1a.1-GFP-WPRE

Plasmid

#211490

Purpose

expresses GFP under artificial promoter Baz1a

Depositor

Georg Keller

Article

O'Toole et al Neuron. 2023 Sep 6:S0896-6273(23)00626-8

Insert

GFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLV_ADRB2-mTurquoise2-iLID

Plasmid

#161002

Purpose

Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to ADRB2 for slow diffusion

Depositor

Sean R Collins

Article

Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

Insert

ADRB2-mTurquoise2-iLID

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-EgI-BlastR

Plasmid

#207176

Purpose

Gateway compatible Lentiviral Expression Plasmid EF1a-IRES-BlastR

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-AP.Agmat.1-GFP-WPRE

Plasmid

#211489

Purpose

expresses GFP under artificial promoter Agmat

Depositor

Georg Keller

Article

O'Toole et al Neuron. 2023 Sep 6:S0896-6273(23)00626-8

Insert

GFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLV_Lyn11-mTurquoise2-iLID

Plasmid

#161000

Purpose

Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to lipidated peptide from LYN

Depositor

Sean R Collins

Article

Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

Insert

Lyn11-mTurquoise2-iLID

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLV_mTurquoise2-iLID-CAAX

Plasmid

#160999

Purpose

Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via C-terminal fusion to CAAX motif from KRAS

Depositor

Sean R Collins

Article

Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

Insert

mTurquoise2-iLID-CAAX

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-EgI-NeoR

Plasmid

#207175

Purpose

Gateway compatible Lentiviral Expression Plasmid EF1a-IRES-NeoR

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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pEFIRES NQO1 WT

Plasmid

#61735

Purpose

Expresses WT human NQO1

Depositor

Yosef Shaul

Article

Asher et al Genes Dev. 2005 Feb 1;19(3):316-21.

Insert

NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)

Use

Tags

None

Expression

Mammalian

Mutation

wild type

Promoter

EF1a

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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pLOX-EWgfp

Plasmid

#12242

Depositor

Didier Trono

Article

Salmon et al Blood. 2000 Nov 15. 96(10):3392-8.

Insert

eGFP

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

EF1a promoter is upstream of GFP. LoxP in 3'…

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pEBB-TB

Plasmid

#36099

Depositor

Ezra Burstein

Article

Maine et al Nat Protoc. 2010 Aug;5(8):1447-59. Epub

Type

Empty backbone

Use

Tags

BT-tag

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 15, 2012

Availability

Academic Institutions and Nonprofits only

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p8RwB

Plasmid

#48733

Purpose

dsRed plasmid

Depositor

John Schiller

Article

Roberts et al Nat Med. 2007 Jul;13(7):857-61. Epub 200

Insert

dsRed-Express

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

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pEFIRES NQO1 Y128F

Plasmid

#61734

Purpose

Expresses human Y128F NQO1

Depositor

Yosef Shaul

Article

Asher et al Genes Dev. 2005 Feb 1;19(3):316-21.

Insert

NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)

Use

Tags

None

Expression

Mammalian

Mutation

Y128F

Promoter

Ef1a

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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pL_FR_Hygro

Plasmid

#127171

Purpose

Frameshift reporter (control plasmid for screening)

Depositor

Paul Blainey

Article

Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

Insert

Frameshift Reporter

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-puro-BGHpA-TRE-pEF-tagRFP-T-NLS

Plasmid

#163083

Purpose

Donor vector for integration of TagRFP-T reporter at AAVS1 safe harbor site via TALENs

Depositor

Lacra Bintu

Article

Van et al Nat Commun. 2021 Jan 22;12(1):537. doi:

Insert

TagRFP-T

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pEFIRES NQO1 Y128V

Plasmid

#61733

Purpose

Expresses human Y128V NQO1

Depositor

Yosef Shaul

Article

Asher et al Genes Dev. 2005 Feb 1;19(3):316-21.

Insert

NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)

Use

Tags

None

Expression

Mammalian

Mutation

Y128V

Promoter

Ef1a

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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pEF.DEST51-YPet

Plasmid

#215541

Purpose

Expression vector with the YPet fluorescent protein

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

YPet

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pEF.DEST51-mTurquoise2

Plasmid

#215539

Purpose

Expression vector with the mTurquoise2 fluorescent protein

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

mTurquoise2

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Zdk3-VPR

Plasmid

#231572

Purpose

Expresses components of the LOOMINA optogenetic transcriptional control system.

Depositor

Dominik Niopek

Article

Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

Insert

Zdk3-VPR, Ef1a driven

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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LEAPVW

Plasmid

#205671

Purpose

shRNAmir backbone for RNA interference, with Venus reporter

Depositor

Dapeng Wang

Article

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pEF-1α-p37-mClover2

Plasmid

#225500

Purpose

Expression of mClover2-tagged p37 protein

Depositor

David Rubinsztein

Article

Wrobel et al Sci Adv. 2024 May 3;10(18):eadl6082. doi

Insert

p37 (UBXN2B Human)

Use

Lentiviral

Tags

mClover2

Expression

Mammalian

Mutation

no

Promoter

EF1A

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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