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We narrowed to 8,867 results for: BLI;

Showing: 3251 - 3300 of 8867 results

  1. pXR003_puroR

    Plasmid
    #219819
    Purpose
    hU6-driven sgRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #109053)
    Depositor
    Philipp Maass
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    219819-plasmid-map-sequence-id-440390

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  2. p-dCas9-VP64-Hygro

    Plasmid
    #104405
    Purpose
    transient expression of dCas9-VP64 fusion protein
    Depositor
    Stephan Beck
    Insert
    VP64
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104405-plasmid-map-sequence-id-204462

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  3. PX380 Treponema denticola Cas9

    Plasmid
    #68317
    Purpose
    Treponema denticola Cas9
    Depositor
    Feng Zhang
    Insert
    TdCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 26, 2015
    Availability
    Academic Institutions and Nonprofits only
    68317-plasmid-map-sequence-id-119529

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  4. PTGR1

    Plasmid
    #38874
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTGR1 (PTGR1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  5. pBHM2641

    Plasmid
    #229525
    Purpose
    Carries OsTIR1-t2a-mNG-AID*, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Use
    CRISPR
    Tags
    t2a-mNeonGreen-AID*
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Promoter
    TEF1
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229525-plasmid-map-sequence-id-459550

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  6. pBHM2647

    Plasmid
    #229989
    Purpose
    Carries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Use
    Tags
    t2a-mNeonGreen-mIAA7_opt
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229989-plasmid-map-sequence-id-461139

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  7. pBHM2648

    Plasmid
    #229990
    Purpose
    Carries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Use
    Tags
    t2a-mNeonGreen-mIAA7_opt
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229990-plasmid-map-sequence-id-461146

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  8. pBHM2649

    Plasmid
    #229991
    Purpose
    Carries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Use
    Tags
    t2a-mNeonGreen-mIAA7_opt
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229991-plasmid-map-sequence-id-461155

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  9. pCer-C3-Plk1-K82R

    Plasmid
    #68134
    Purpose
    Expression of Cerulean-tagged, kinase-dead Plk1 in mammalian cells
    Depositor
    Catherine Lindon
    Insert
    Polo-like kinase 1 (PLK1 Human)
    Use
    Tags
    Cerulean
    Expression
    Mammalian
    Mutation
    K82R kinase-dead version
    Promoter
    CMV
    Available since
    Oct. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    68134-plasmid-map-sequence-id-123466

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  10. m.3047-Left TALE-G1397-N-DddA11-mCherry

    Plasmid
    #211336
    Purpose
    Expression of codon-optimized mitochondrial base editor in human cells; cell sorting; blasticidin drug selection
    Depositor
    Monkol Lek
    Insert
    m.3047-Left TALE-G1397-N-DddA11-mCherry
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    211336-plasmid-map-sequence-id-415645

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  11. p426TEF-LptTPS-A

    Plasmid
    #89468
    Purpose
    Expression of prespatane synthase LptTPS-A in S. cerevisiae for sesquiterpene production
    Depositor
    Jing-Ke Weng
    Insert
    LptTPS-A
    Use
    Tags
    none
    Expression
    Yeast
    Mutation
    Promoter
    pBR322 origin of replication for propagation in E…
    Available since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    89468-plasmid-map-sequence-id-176087

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  12. pJK407

    Plasmid
    #72421
    Purpose
    Produces Acetobacter aceti 1023 Est06, C-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    lipase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    lacks Met1 and Thr2 in annotated (v1) sequence
    Promoter
    T7
    Available since
    Feb. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    72421-plasmid-map-sequence-id-134675

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  13. pAAV-CaMKIIa-HA-rM3D(Gs)-IRES-mCitrine

    Plasmid
    #50468
    Purpose
    Gs-coupled rM3D-IRES-mCitrine under the control of CaMKIIa promoter
    Depositor
    Bryan Roth
    Insert
    rM3D-IRES-mCitrine
    Use
    AAV
    Tags
    HA
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    CaMKIIa
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50468-plasmid-map-sequence-id-75484

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  14. pmSod1-G93A-N’His

    Plasmid
    #117705
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Elizabeth Fisher
    Insert
    Sod1 (Sod1 Mouse)
    Use
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    G93A
    Promoter
    T7
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    117705-plasmid-map-sequence-id-227526

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  15. pShuttle-CMV-F2A-T2A-Venus

    Plasmid
    #62621
    Purpose
    AdEasy Shuttle vector modified for multicistronic expression. Contains T2A, F2A and Venus tag
    Depositor
    Zheng-Xu Wang
    Insert
    MCS with F2A and T2A
    Use
    Adenoviral
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62621-plasmid-map-sequence-id-102957

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  16. pcDNA3.1_C-6His-Stx17

    Plasmid
    #112259
    Purpose
    C-term His tagged pcDNA3.1 vector expressing mouse Stx17 (syntaxin 17)
    Depositor
    Madhulika Tripathi
    Insert
    syntaxin 17 (Stx17 Mouse)
    Use
    Tags
    6His Tag
    Expression
    Mammalian
    Mutation
    Promoter
    T7
    Available since
    July 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    112259-plasmid-map-sequence-id-217085

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  17. 371-GFP

    Plasmid
    #97030
    Purpose
    His6-GFP-tev-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Use
    Tags
    GFP fusion, His6 tag, and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97030-plasmid-map-sequence-id-182506

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  18. pSG5-Crkl mut SH2/SH3

    Plasmid
    #41704
    Depositor
    Nora Heisterkamp
    Insert
    Crkl (CRKL Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    R39K and W160K
    Promoter
    Available since
    April 10, 2013
    Availability
    Academic Institutions and Nonprofits only

  19. pJK234

    Plasmid
    #73324
    Purpose
    Produces Janthinobacterium lividum violacein biosynthesis proteins VioA, VioB, and VioC
    Depositor
    T. J. Kappock
    Inserts
    violacein biosynthesis protein VioA
    violacein biosynthesis protein VioB
    violacein biosynthesis protein VioC
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7 and n/a
    Available since
    March 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    73324-plasmid-map-sequence-id-135032

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  22. pKT-mPDGFb-IRES-Katushka

    Plasmid
    #133082
    Purpose
    Expresses mouse PDGFb and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.
    Depositor
    Maria Castro
    Insert
    PDGFb (Pdgfb Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    133082-plasmid-map-sequence-id-261113

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  23. pCas9CR5-NG

    Plasmid
    #120228
    Purpose
    pCas9CR5 with mutations to change PAM site specificity to NG from Nishimasu et al.
    Depositor
    Christopher Reisch
    Insert
    SpCas9-NG
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Mutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…
    Promoter
    pTet
    Available since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120228-plasmid-map-sequence-id-233263

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  24. PRDX4

    Plasmid
    #38851
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PRDX4 (Prdx4 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  25. pSG5-Crkl mut SH2

    Plasmid
    #41703
    Depositor
    Nora Heisterkamp
    Insert
    Crkl (CRKL Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    R39K
    Promoter
    SV40
    Available since
    April 9, 2013
    Availability
    Academic Institutions and Nonprofits only

  26. m.5147-Left TALE-G1397-N-DddA11-mCherry

    Plasmid
    #211338
    Purpose
    Expression of codon-optimized mitochondrial base editor in human cells; cell sorting; blasticidin drug selection
    Depositor
    Monkol Lek
    Insert
    m.5147-Left TALE-G1397-N-DddA11-mCherry
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    211338-plasmid-map-sequence-id-415660

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  27. pUS252y

    Plasmid
    #191834
    Purpose
    Expresses fuYFP constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuYFP to place in other plasmid backbones.
    Depositor
    Nicholas Coleman
    Insert
    fuYFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 17, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    191834-plasmid-map-sequence-id-382126

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  28. pBS L30 mRuby3-Rab8A

    Plasmid
    #166428
    Purpose
    Rab8A fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.
    Depositor
    Raphael Gaudin
    Insert
    Rab8A
    Use
    Tags
    mRuby3
    Expression
    Mammalian
    Mutation
    Promoter
    L30 weak promoter
    Available since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    166428-plasmid-map-sequence-id-333537

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  30. pet15b_ack

    Plasmid
    #220229
    Purpose
    Bacterial expression of AcK in E. coli BL21_DE3 (pLys) or similar
    Depositor
    Bernd Nidetzky
    Insert
    Acetate kinase (ackA )
    Use
    Tags
    Histag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    220229-plasmid-map-sequence-id-436823

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  31. katushka-FMDV-P2A-pTRIP

    Plasmid
    #163522
    Purpose
    Used to construct lentivirus to express katushka in mammalian cells with option for a second insert
    Depositor
    Thomas Weber
    Insert
    stopless Katushka-P2A
    Use
    Lentiviral
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    163522-plasmid-map-sequence-id-321575

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  32. PB CAG-L-bpA-2xSV40pA-L-tdGFP-Lrig1_shRNAmir_3

    Plasmid
    #178049
    Purpose
    CAG tdGFP Lrig1 shRNAmir-3 cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Lrig1 shRNAmir
    Use
    Cre/Lox and RNAi; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    178049-plasmid-map-sequence-id-352988

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  33. PB CAG-L-bpA-2xSV40pA-L-tdGFP-Lrig1_shRNAmir_2

    Plasmid
    #178048
    Purpose
    CAG tdGFP Lrig1 shRNAmir-2 cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Lrig1 shRNAmir
    Use
    Cre/Lox and RNAi; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    178048-plasmid-map-sequence-id-352985

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  34. PB CAG-L-bpA-2xSV40pA-L-tdGFP-Lrig1_shRNAmir_1

    Plasmid
    #178047
    Purpose
    CAG tdGFP Lrig1 shRNAmir-1 cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Lrig1 shRNAmir
    Use
    Cre/Lox and RNAi; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    178047-plasmid-map-sequence-id-352935

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  35. pCMV GATA2

    Plasmid
    #83820
    Purpose
    Mammalian expression of mouse Gata2
    Depositor
    Douglas Engel
    Insert
    GATA2 (Gata2 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 3, 2016
    Availability
    Academic Institutions and Nonprofits only

  36. pAAV-synP-FLEX-TVA66T-EGFP

    Plasmid
    #64096
    Purpose
    Expresses TVA66T-EGFP under the synapsin promotor
    Depositor
    Ian Wickersham
    Insert
    TVA66T-EGFP
    Use
    AAV and Cre/Lox
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Glutamic acid 66 to Threonine
    Promoter
    synapsin
    Available since
    April 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    64096-plasmid-map-sequence-id-107236

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  37. pNHA_OROV_NCP

    Plasmid
    #208937
    Purpose
    Expresses N-terminally HA-tagged Oropouche orthobunyavirus nucleocapsid protein from CMV promoter in mammalian cells
    Depositor
    Gerald Barry
    Insert
    Nucleocapsid
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Deleted start codon so HA included at N-terminus
    Promoter
    CMV
    Available since
    Jan. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    208937-plasmid-map-sequence-id-418963

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  38. BA.2.86

    Plasmid
    #208583
    Purpose
    Mammalian expression vector for expressing BA.2.86 SARS-CoV-2 Spike, with 19AA cytoplasmic tail truncation
    Depositor
    Ben Murrell
    Insert
    SARS-CoV-2 variant BA.2.86 spike (S )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    208583-plasmid-map-sequence-id-409580

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  40. FLAG-hMYF5 (#795)

    Plasmid
    #78333
    Purpose
    Overexpression of human MYF5 ORF with a N-terminal FLAG tag
    Depositor
    Matthew Alexander, Louis Kunkel
    Insert
    MYF5 (MYF5 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    78333-plasmid-map-sequence-id-373227

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  41. pAAV-CAG-mNeonGreen

    Plasmid
    #124101
    Purpose
    Expression of the fluorescent protein mNeonGreen in mammalian cells
    Depositor
    Pantelis Tsoulfas
    Insert
    mNeonGreen
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Codon usage was optimized for Homo Sapiens
    Promoter
    CAG
    Available since
    April 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124101-plasmid-map-sequence-id-241203

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  42. PTPN5

    Plasmid
    #39169
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTPN5 (PTPN5 Human)
    Use
    Tags
    GST-PreScission
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  43. pEGFPN1‐mTim1

    Plasmid
    #49215
    Purpose
    expresses EGFP tagged mouse Tim1
    Depositor
    Lawrence Kane
    Insert
    Tim1 (Havcr1 Mouse)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 15, 2014
    Availability
    Academic Institutions and Nonprofits only

  44. p72B9-FBL-HC

    Plasmid
    #194637
    Purpose
    Expresses 72B9 hybridoma anti-FBL antibody, heavy chain
    Depositor
    Susan Baserga
    Insert
    72B9 antibody, heavy chain
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    194637-plasmid-map-sequence-id-382496

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  45. p72B9-FBL-LC

    Plasmid
    #194638
    Purpose
    Expresses 72B9 hybridoma anti-FBL antibody, light chain
    Depositor
    Susan Baserga
    Insert
    72B9 antibody, light chain
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    194638-plasmid-map-sequence-id-382497

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  46. pACP-tag (m)- 2 Vector

    Plasmid
    #101126
    Purpose
    Mammalian expression plasmid that allows for the expression of a gene of interest fused to the N-terminus or C-terminus of ACP-tag.
    Depositor
    New England Biolabs, Ana Egana
    Type
    Empty backbone
    Use
    Tags
    ACP-tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 27, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101126-plasmid-map-sequence-id-194174

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  47. pJR255

    Plasmid
    #78549
    Purpose
    For expressing small noncoding RNAs from U6 promoter. One step cloning of oiigonucleotide pairs containing CACC and AAAA overhangs. CMV driven mCherry visible marker.
    Depositor
    John Rossi
    Type
    Empty backbone
    Use
    RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6, CMV
    Available since
    Jan. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    78549-plasmid-map-sequence-id-191088

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  48. pcDNA3.3_SARS2_EG.5.1

    Plasmid
    #207641
    Purpose
    pseudovirus plasmid that expresses spike of EG.5.1
    Depositor
    David Nemazee
    Insert
    Spike (EG.5.1) (S SARS-CoV-2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Q52H, F456L on the basis of XBB.1.5
    Promoter
    CMV
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    207641-plasmid-map-sequence-id-437731

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  49. mT2A-tdTomato-FNF

    Plasmid
    #182340
    Purpose
    Recombineering cassette plasmid for T2A-tdTomato-FNF
    Depositor
    Mario Capecchi
    Insert
    T2A-tdTomato-FNF
    Use
    Mouse Targeting; Flp / frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182340-plasmid-map-sequence-id-358215

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  50. pMCP-tag (m) Vector

    Plasmid
    #101127
    Purpose
    Mammalian expression plasmid that allows for the expression of a gene of interest fused to the N-terminus or C-terminus of MCP-tag.
    Depositor
    New England Biolabs, Ana Egana
    Type
    Empty backbone
    Use
    Tags
    MCP-tag (Mutant ACP- tag)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
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