We narrowed to 117,330 results for: CIL

1. pAB078d7

   Plasmid

   #79205

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PlacZ-opt (off-target) luxAB; Ppro1 434cI-SH2ABL1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PlacZ-opt (off-target); Ppro1

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAB085e

   Plasmid

   #79212

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PlacZ-opt (OR1) luxAB; Ptet 434cI-TnTBR3-F7
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PlacZ-opt (OR1); Ptet

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJK520

   Plasmid

   #155386

   Purpose

   PphlF-TA1 crRNAs, 2x cascade. dCas12a (D917A) + PA4-mVenus

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Inserts

   dCas12a (F. novicida)
   PA4-mVenus
   PphlF-TA1 in dCas12a oscillator crRNAs
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   D917A (nuclease-deactivating)

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLIB-GST-TEV-RSP3(160-516)

   Plasmid

   #176692

   Purpose

   Expression of Chlamydomonas reinhardtii RSP3 recombinant protein in insect cells

   Depositor

   Ron Vale

   Article

   Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

   Insert

   RSP3
   

   Use

   Tags

   GST

   Expression

   Insect

   Mutation

   None

   Promoter

   polyhedrin

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLentiRNAGuide_001 - hU6-RfxCas13d-DR1-BsmBI-EFS-Puro-WPRE

   Plasmid

   #138150

   Purpose

   For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   hU6_Rfxcas13d-DR1_BsmBI
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   DR1 A>T

   Promoter

   Available since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC57-mitoLbNOX

   Plasmid

   #74448

   Purpose

   This plasmid contains human codon optimized sequence of mitoLbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.

   Depositor

   Vamsi Mootha

   Article

   Titov et al Science. 2016 Apr 8;352(6282):231-5. doi

   Insert

   mitoLbNOX
   

   Use

   Tags

   Flag tag with linker and mitochondrial targeting …

   Expression

   Mutation

   Human codon optimized

   Promoter

   Available since

   June 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV[Exp]-Puro-CMV>{5-HT6-R_GECO1.1}

   Plasmid

   #232879

   Purpose

   Expresses the 5-HT6R coupled to the RGECO1 calcium sensor

   Depositor

   Paul DeCaen

   Article

   DeCaen et al J Cell Physiol. 2025 Apr;240(4):e70034.

   Insert

   5-Hydroxytryptamine Receptor 6 (HTR6 Human)
   

   Use

   Lentiviral

   Tags

   RGECO1.1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV[Exp]-Puro-CMV>{ARL13B_6gly_GFP_6Gly_R_GECO1}

   Plasmid

   #232880

   Purpose

   Expresses the ARL13B coupled to GFP and RGECO1 calcium sensor.

   Depositor

   Paul DeCaen

   Article

   DeCaen et al J Cell Physiol. 2025 Apr;240(4):e70034.

   Insert

   ADP-ribosylation factor-like protein 13B (ARL13B Human)
   

   Use

   Lentiviral

   Tags

   GFP and RGECO1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLentiRNACRISPR_007 - TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

   Plasmid

   #138149

   Purpose

   Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   CasRx
   

   Use

   Lentiviral

   Tags

   NLS-HA

   Expression

   Mutation

   Promoter

   Available since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNCS-Antares

    Plasmid

    #74279

    Purpose

    Constitutive expression of Antares in bacteria

    Depositor

    Michael Lin

    Article

    Chu et al Nat Biotechnol. 2016 May 30. doi: 10.103

    Insert

    Antares
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGGS-PKCdelta-EGFP

    Plasmid

    #178856

    Purpose

    Mammalian expression of PKCδ fused to EGFP

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    PKCδ (PRKCD Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQCXIP-BSR-GFP11

    Plasmid

    #68716

    Purpose

    Split GFP assay

    Depositor

    Yutaka Hata

    Article

    Kodaka et al Exp Cell Res. 2015 Aug 15;336(2):171-81.

    Insert

    bsr
    

    Use

    Retroviral

    Tags

    FLAG and GFP11

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT077

    Plasmid

    #137879

    Purpose

    Donor vector for integration into the human AAVS1 safe harbor locus of Doxicycline-inducible KRAB-dCas9-IRES-EGFP

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    TRE3G-KRAB-dCas9-IRES-EGFP-CAG-TetON-NeoR-SA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLentiRNACRISPR_008-TetO-NLS-hfCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

    Plasmid

    #223173

    Purpose

    Expresses hfCas13d (N2V8) in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus.

    Depositor

    Neville Sanjana

    Article

    Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

    Insert

    hfCas13d (N2V8)
    

    Use

    Lentiviral

    Tags

    NLS-HA

    Expression

    Mutation

    Promoter

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pB-MultiplexedReporter-MOR170-1

    Plasmid

    #129461

    Purpose

    piggyBac transposon vector encoding the receptor MOR170-1 and the barcoded CRE reporter gene

    Depositor

    Sriram Kosuri

    Article

    Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

    Insert

    MOR170-1 (Olfr895 Mouse)
    

    Use

    Synthetic Biology

    Tags

    LUCY tag, Rho tag

    Expression

    Mammalian

    Mutation

    Promoter

    TRE (Tet-On)

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT076

    Plasmid

    #137880

    Purpose

    Donor vector for integration at the human AAVS1 safe harbor locus of Doxicycline-inducible dCas9-VPR-T2A-EGFP

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    TRE3G-dCas9-VPR-T2A-EGFP-CAG-TetON-NeoR-SA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCRISPRyl

    Plasmid

    #70007

    Purpose

    CRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertion

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2015 Dec 29.

    Inserts

    Codon optimized Cas9 from S. pyogenes
    sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SCR1'-tRNA and UAS1B8-TEF(136)

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLentiRNAGuide_002 - hU6-RfxCas13d-DR-BsmBI-EFS-Puro-WPRE

    Plasmid

    #138151

    Purpose

    For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    hU6_Rfxcas13d-DR_BsmBI
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMRX-IB-mGFP-FTH1

    Plasmid

    #192800

    Purpose

    Stable expression of mGFP-FTH1 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

    Insert

    FTH1 (FTH1 Human)
    

    Use

    Tags

    monomeric EGFP (A206K)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GFP-PS9

    Plasmid

    #177944

    Purpose

    Transient mammalian expression of eGFP along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    eGFP (ORF1ab )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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