We narrowed to 2,463 results for: T2A

1. pBZ191-pU6-sgBFP-CBh-sv40NLS-Cas9-NLS-T2A-mCherry

   Plasmid

   #170183

   Purpose

   Expression vector for a sgRNA against BFP and spCas9 linked to mCherry via a T2A peptide

   Depositor

   Hunter Fraser

   Article

   Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris

   Insert

   sgBFP
   

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. 874U=ExuL-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

   Plasmid

   #159671

   Purpose

   Plasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

   Depositor

   Omar Akbari

   Article

   Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

   Insert

   ExuL-NLS-hSpCas9-T2A-GFP
   

   Tags

   T2A-GFP

   Expression

   Insect

   Available Since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. NGN3-T2A-NLS-tdTomato-ires-puro-BGHpA-PGK-puro

   Plasmid

   #89994

   Purpose

   Donor template for generation of NGN3-ntdTomato reporter cell lines

   Depositor

   Timo Otonkoski

   Insert

   NGN3-T2A-NLS-TdTomato-ires-puro-BGHpA-PGK-puro (NEUROG3 Human)
   

   Use

   CRISPR

   Promoter

   none

   Available Since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. 1095=Rcd-1r-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

   Plasmid

   #159673

   Purpose

   Plasmid supports Cas9 expression in both males and females, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

   Depositor

   Omar Akbari

   Article

   Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

   Insert

   Rcd-1r-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40
   

   Tags

   T2A-GFP

   Expression

   Insect

   Available Since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC19-GCG-T2A-NLS-EmGFP-P2A-puro-PGK-puro

   Plasmid

   #121175

   Purpose

   Donor template for CRISPR-Cas9 mediated generation of GCG-NLS-EmGFP reporter cell lines

   Depositor

   Timo Otonkoski

   Insert

   GCG-T2A-NLS-EmGFP-P2A-Puro-PGK-Puro
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   July 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGP-20XUAS-IVS-Syn21-op1-jGCaMP7s-T2A-tdTomato-p10

   Plasmid

   #172916

   Purpose

   Gal4 activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Insert

   jGCaMP7s-T2A-tdTomato
   

   Tags

   6xHis and T2A-tdTomato

   Expression

   Insect

   Promoter

   hsp70

   Available Since

   Aug. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. XZ066-(PB)EF1a-LIDARST-CCR6V2-IRESv0-T2A-PuroR-P2A-BFP

   Plasmid

   #233418

   Purpose

   PiggyBac donor plasmid with CCR6-V2-ADAR2dd(E488Q,T501A) and ARRB2-tdMCP separated by EMCV IRES

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   CCR6-ADARdd-P2A-PuroR-IRES-ARRB2-MCP
   

   Expression

   Mammalian

   Mutation

   E488Q, T501A on ADAR2dd

   Promoter

   EF1a

   Available Since

   June 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRCA376 - pBA904 Puro-T2A-GFP NTC guide (pRCA360 backbone)

   Plasmid

   #238167

   Purpose

   Lentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

   Depositor

   Thomas Norman

   Article

   Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

   Insert

   Non-targeting sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   GFP

   Available Since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPB-[EF1a-mRuby2-bGH]-UbC-PuroR-T2A-mGL-SV40pA

   Plasmid

   #235325

   Purpose

   PiggyBac integration vector for ComMAND base gene regulating mRuby2

   Depositor

   Kate Galloway

   Article

   Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

   Insert

   mRuby2
   

   Use

   Synthetic Biology; Piggybac

   Expression

   Mammalian

   Promoter

   EF1a (human EF1a)

   Available Since

   May 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-eCas9-T2A-EGFP-U6-gRNA targeting Nrl-no ITR and f1 ori

    Plasmid

    #234737

    Purpose

    All-in-one CRISPR/Cas9 vector encodes high-fidelity eSpCas9 and a gRNA targeting Nrl, efficiently reprogramming rod precursors into cone-like cells in the mouse retina.

    Depositor

    Kirill Martemyanov

    Article

    Zhao et al Nat Commun. 2025 Apr 22;16(1):3768. doi:

    Insert

    Nrl (Nrl Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV hU6-VEGFR2#3 sgRNA Nestin-dCas9-KRAB-T2a-GFP

    Plasmid

    #196990

    Purpose

    Derived from pLV hU6-sgRNA Nestin-dCas9-KRAB-T2a-GFP with KDR sgRNA

    Depositor

    Elizabeth Kirby

    Article

    Dause et al Mol Neurobiol. 2025 Mar 25. doi: 10.1007

    Insert

    VEGFR2
    

    Use

    CRISPR and Lentiviral

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. Const.6 EGFP-P2A-dTomato-T2A-TagBFP-IRES-Luc-PS9

    Plasmid

    #231910

    Purpose

    For packaging EGFP, dTomato, TagBFP and firefly luciferase mRNA into SARS-CoV-2 virus-like particles (VLPs)

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Insert

    EGFP-P2A-dTomato-T2A-TagBFP-IRES-Luc
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-EF1a-NLS-msfGFP-T2A-mCherry-NES> mPGK-PuroR

    Plasmid

    #218916

    Purpose

    Lentiviral construct expressing msfGFP and nuclear mCherry proteins.

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    EF1a-msfGFP-T2A-mCherry-NLS > mPGK-PuroR
    

    Use

    Lentiviral

    Available Since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJT256 Lenti pEF-dCas9-3XFLAG-N+F-T2A-tagBFP-P2A-Blast

    Plasmid

    #187332

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCas9-3XFLAG-N+F-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJT257 Lenti pEF-dCas9-3XFLAG-Z+F-T2A-tagBFP-P2A-Blast

    Plasmid

    #187333

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCas9-3XFLAG-Z+F+N-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJT255 Lenti pEF-dCas9-3XFLAG-N+Z-T2A-tagBFP-P2A-Blast

    Plasmid

    #187331

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCas9-3XFLAG-N+Z-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJT331 Lenti pEF-dCas9-3XFLAG-Q+N+Z+F-T2A-tagBFP-P2A-Blast

    Plasmid

    #195538

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Lenti pEF-dCas9-3XFLAG-Q+N+Z+F-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTol2-nbt300 2n-KalNFB-3xmir1T-syt2a-10U2n-mCardinal-P2A-mClover3

    Plasmid

    #196117

    Purpose

    zebrafish expression

    Depositor

    Reinhard Köster

    Insert

    nbt300 2n-KalNFB-3xmir1T-syt2a-HO1-10U2n-mCardinal-P2A-mClover3
    

    Use

    Zebrafish expression

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmCherry2-GNB1-T2A-mCherry2-GNG2-IRES-GNAI1-mEYFP(Q69K)

    Plasmid

    #190755

    Purpose

    Expression of trimeric G protein with mCherry2 (GNB1 and GNG2) or mEYFP(Q69K) (GNAI1) tags.

    Depositor

    Dave Piston

    Article

    Foust et al bioRxiv 2021.11.03.467169

    Inserts

    GNB1 (GNB1 Human)
    GNG2 (GNG2 Human)
    GNAI1 (GNAI1 Human)
    

    Tags

    mCherry2 and mEYFP(Q69K)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(PAMmut)

    Plasmid

    #176139

    Purpose

    EGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    XRCC1-T2A-PolB (XRCC1, POLB Human)
    

    Use

    Lentiviral

    Tags

    EGFP and MYC

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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