We narrowed to 5,295 results for: pcDNA3.1+

1. pcDNA3.1(+)/AUG-DAP5-3XFLAG

   Plasmid

   #127331

   Purpose

   Express 3xFLAG tagged DAP5 protein from AUG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   DAP5 (EIF4G2 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3.1 (-) + SLNCR1 + 12x MS2 bs

   Plasmid

   #86828

   Purpose

   Expression of SLNCR1 lncRNA tagged with 12 copies of the MS2 stem-loop sequence

   Depositor

   Carl Novina

   Article

   Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

   Insert

   LINC00673 (LINC00673 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-FLT3-D835Y-V5/HIS

   Plasmid

   #236007

   Purpose

   expression of the D835Y kinase defective mutant variant of human FLT3 that is associated with Acute Myeloid Leukemia and Acute Lymphoblastic Leukemia

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   human FLT3-D835Y receptor tyrosine kinase, full length (FLT3 Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   D835Y substitution

   Promoter

   CMV

   Available since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-FLT3-D835N-V5/HIS

   Plasmid

   #236008

   Purpose

   expression of the D835N kinase defective mutant variant of human FLT3 that is associated with Acute Myeloid Leukemia

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   human FLT3-D835N receptor tyrosine kinase, full length (FLT3 Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   D835N substitution

   Promoter

   CMV

   Available since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. XE251 pCDNA3.1(zeo)-hDsh3

   Plasmid

   #16758

   Depositor

   Randall Moon

   Article

   Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

   Insert

   FLAG-hDsh3 (DVL3 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 13, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-CD9-(TEV)-tTA

   Plasmid

   #162596

   Purpose

   human CD9-TEV cleavage site-tTA

   Depositor

   Masaharu Somiya

   Article

   Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

   Insert

   human CD9 (CD9 Human)
   

   Use

   Tags

   TEV protease cleavage site and tTA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-3xFLAG-TEV-UBE2O

   Plasmid

   #105718

   Purpose

   Expresses WT full length human N- terminally tagged UBE2O

   Depositor

   Ramanujan Hegde

   Article

   Yanagitani et al Science. 2017 Aug 4;357(6350):472-475. d

   Insert

   UBE2O (UBE2O Human)
   

   Use

   Tags

   3xFLAG-TEV

   Expression

   Mammalian

   Mutation

   Promoter

   CMV (from vector)

   Available since

   Feb. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1_Zeo_ARRB2-1-383-CTEV-2xHA

   Plasmid

   #194382

   Purpose

   Split TEV assays

   Depositor

   Michael Wehr

   Article

   Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

   Insert

   ARRB2-CTEV-2xHA (ARRB2 Synthetic, Human)
   

   Use

   Tags

   2xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 LIC 6A CARD8-FLAG

   Plasmid

   #169948

   Purpose

   Mammalian expression of CARD8-FLAG

   Depositor

   Hao Wu

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA3.1- dCas9-2xNLS-EGFP

    Plasmid

    #74710

    Purpose

    Expresses dCas9-2xNLS-EGFP in mammalian cells

    Depositor

    Eugene Yeo

    Article

    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

    Insert

    dCas9-2xNLS-EGFP
    

    Use

    CRISPR

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-FLAG-SBP-STAG2 (WT)

    Plasmid

    #73963

    Purpose

    STAG2 cDNA (CCDS43990) wild type

    Depositor

    Todd Waldman

    Article

    Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

    Insert

    STAG2 (STAG2 Human)
    

    Use

    Tags

    FLAG-SBP

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3.1-MS2-HA-HsStaufen2-T1_V

    Plasmid

    #147741

    Purpose

    Mammalian Expression of HsStaufen2-T1

    Depositor

    Elisa Izaurralde

    Insert

    HsStaufen2-T1 (STAU2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCherry-2A-eGFP(W58X) pcDNA3.1(-)

    Plasmid

    #172229

    Purpose

    Expresses an mCherry-2A-eGFP dual reporter that contains a UAG codon in position 58 (W58X) of eGFP, in mammalian cells

    Depositor

    Joshua Rosenthal

    Article

    Montiel-Gonzalez et al Nucleic Acids Res. 2016 Dec 1;44(21):e15

    Insert

    mCherry-2A-eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-HaloEKAR2.2-T2A-EGFP

    Plasmid

    #216495

    Purpose

    Expression of chemigenetic ERK biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells

    Depositor

    Jin Zhang

    Article

    Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

    Insert

    HaloEKAR2.2
    

    Use

    Tags

    T2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1+-mutTornado-split-nLuc

    Plasmid

    #212707

    Purpose

    Tornado split nLuc with a mutated 3' ribozyme so that circularization cannot occur.

    Depositor

    Samie Jaffrey

    Article

    Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

    Insert

    mutTornado-CVB3-split-nLuc
    

    Use

    Tags

    Expression

    Mutation

    Deleted the partial 3'ribozyme sequence in T…

    Promoter

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-hRIPK1-Flag-delC

    Plasmid

    #112493

    Purpose

    Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.

    Depositor

    Junying Yuan

    Insert

    RIPK1 (RIPK1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deletion of C terminus (AA290-671 deleted)

    Promoter

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-myc-BirA*-KRASG12V

    Plasmid

    #122208

    Purpose

    Expresses myc-BirA* fused to KRASG12V in mammalian cells

    Depositor

    Christopher Counter

    Article

    Adhikari et al Nat Commun. 2018 Sep 7;9(1):3646. doi: 1

    Insert

    KRASG12V (KRAS Human)
    

    Use

    Tags

    myc-BirA*

    Expression

    Mammalian

    Mutation

    changed G12 to V

    Promoter

    CMV

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-dCas9-MQ1(WT)-EGFP

    Plasmid

    #89633

    Purpose

    Targeted CpG methylation

    Depositor

    Margaret Goodell

    Article

    Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

    Insert

    site-specific DNA-methyltransferase SssI
    

    Use

    CRISPR

    Tags

    3*FLAG and 6*His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-CibN-dCas9-CibN

    Plasmid

    #60553

    Purpose

    Expresses CibN-dCas9-CibN in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

    Insert

    dCas9-CibN
    

    Use

    CRISPR

    Tags

    FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    inactivating Cas9 mutations D10A and H840A

    Promoter

    CMV

    Available since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-CHRM3-cpHalo∆-mEGFP

    Plasmid

    #194678

    Purpose

    CMV driven expression of the m3 GPCR recorder in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    CHRM3_cpHalo∆
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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