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We narrowed to 2,018 results for: cas9 expression vector

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Showing: 1981 - 2010 of 2018 results

  2. pYPQAT16

    Plasmid
    #223388
    Purpose
    T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for monocot plants; NGG PAM; wide working window; A3A/Y130F-CBE_V01 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-hA3A-Y130F-SpCas9-D10A-UGI-OsU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    May 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    223388-plasmid-map-sequence-id-474023

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  3. pYPQAT26

    Plasmid
    #223398
    Purpose
    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for dicot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by ZmUbi1 and the sgRNA was driven by AtU3; BASTA for plants select.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-AtU3-gRNA scaffold 2.0
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223398-plasmid-map-sequence-id-452310

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  4. pYPQAT29

    Plasmid
    #223401
    Purpose
    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for monocot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by ZmUbi1 and the sgRNA was driven by OsU3; BASTA for plant select.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-OsU3-gRNA scaffold 2.0
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223401-plasmid-map-sequence-id-452216

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  5. pYPQAT30

    Plasmid
    #223402
    Purpose
    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for monocot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 and the sgRNA was driven by separate ZmUbi1; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-ZmUbi-gRNA scaffold 2.0-tRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223402-plasmid-map-sequence-id-454247

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  6. pYPQAT14

    Plasmid
    #223386
    Purpose
    T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for dicot plants; NGG PAM; wide working window; A3A/Y130F-CBE_V01 was driven by ZmUbi1 and sgRNA was by AtU3; BASTA for plant selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-hA3A-Y130F-SpCas9-D10A-UGI-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223386-plasmid-map-sequence-id-454178

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  7. pPEPZ-sgRNAclone

    Plasmid
    #141090
    Purpose
    This vector is designed for efficient cloning of sgRNAs by Golden Gate assembly. The sgRNA insertion leads to replacement of mCherry, resulting in loss of red color of E. coli colony.
    Depositor
    Jan-Willem Veening
    Article
    Liu et al Cell Host Microbe. 2021 Jan 13;29(1):107
    Insert
    mCherry
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    p3
    Available Since
    April 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    141090-plasmid-map-sequence-id-330475

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  12. pMT1-13X-gRNA1-2(AtUBI10)-MTAP-LUC

    Plasmid
    #234370
    Purpose
    T-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs
    Depositor
    Michael Smanski
    Article
    Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70
    Inserts
    NbGP41-sfGFP-VP64-GB1
    Luciferase
    dCas9-13XGP41
    gRNA 1-1 and gRNA 1-2
    Use
    Synthetic Biology
    Tags
    GB1, GP41, and sfGFP
    Expression
    Plant
    Promoter
    Arabidopsis U6, Arabidopsis Ubi10, and MTAP1 (syn…
    Available Since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    234370-plasmid-map-sequence-id-469430

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  13. pMT1-13X-NOG(AtUBI10)-MTAP-LUC

    Plasmid
    #234371
    Purpose
    T-DNA vector for expression of the the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs, no gRNA included (NOG)
    Depositor
    Michael Smanski
    Article
    Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70
    Inserts
    NbGP41-sfGFP-VP64-GB1
    Luciferase
    dCas9-13XGP41
    Use
    Synthetic Biology
    Tags
    GB1, GP41, and sfGFP
    Expression
    Plant
    Promoter
    Arabidopsis Ubi10 and MTAP1 (synthetic)
    Available Since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    234371-plasmid-map-sequence-id-469425

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  14. pMT1-24X-gRNA1-2(AtUBI10)-MTAP-LUC

    Plasmid
    #234372
    Purpose
    T-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs
    Depositor
    Michael Smanski
    Article
    Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70
    Inserts
    NbGP41-sfGFP-VP64-GB1
    Luciferase
    dCas9-24XGP41
    gRNA 1-1 and gRNA 1-2
    Use
    Synthetic Biology
    Tags
    GB1, GP41, and sfGFP
    Expression
    Plant
    Promoter
    Arabidopsis U6, Arabidopsis Ubi10, and MTAP1 (syn…
    Available Since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    234372-plasmid-map-sequence-id-469423

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  18. pRN3P_T3_ABE8e_IVT

    Plasmid
    #201676
    Purpose
    Plasmid to be used as DNA template for in vitro RNA transcription of the ABE8e base editor (A to G) by T3 RNA polymerase. The plasmid contains optimised 5'UTR and 3'UTR to improve protein expression.
    Depositor
    Timo Otonkoski
    Article
    Jalil et al Am J Hum Genet. 2024 Apr 4;111(4):714-72
    Insert
    ecTadA(8e)-nSpCas9
    Use
    CRISPR; Vector for in-vitro transcription
    Promoter
    T3 promoter
    Available Since
    April 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    201676-plasmid-map-sequence-id-406944

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  19. lentiMutB3GNT5-blast

    Plasmid
    #208380
    Purpose
    lentiviral vector for expressing human B3GNT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence
    Depositor
    Jennifer Kohler
    Article
    Ghorashi et al bioRxiv [Preprint]. 2023 Aug 3:2023.08.0
    Insert
    Beta-1,3-N-Acetylglucosaminyltransferase 5 (B3GNT5 Human)
    Use
    Lentiviral
    Tags
    myc, FLAG
    Expression
    Mammalian
    Mutation
    nt 922 c->g (PAM site inactivation)
    Promoter
    EF-1 alpha core promoter
    Available Since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    208380-plasmid-map-sequence-id-414094

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  20. lentiMutB3GALT5-blast

    Plasmid
    #208381
    Purpose
    lentiviral vector for expressing human B3GALT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence
    Depositor
    Jennifer Kohler
    Article
    Ghorashi et al bioRxiv [Preprint]. 2023 Aug 3:2023.08.0
    Insert
    beta-1,3-galactosyltransferase 5 (B3GALT5 Human)
    Use
    Lentiviral
    Tags
    myc, FLAG
    Expression
    Mammalian
    Mutation
    nucleotide change in PAM targeting sequence nt 51…
    Promoter
    EF-1 alpha core promoter
    Available Since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    208381-plasmid-map-sequence-id-414093

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  25. AAVS1-iSAM

    Plasmid
    #211495
    Purpose
    AAVS1 targeting vector containing an all-in-one DOX-inducible system for CRISPRa
    Depositor
    Antonella Fidanza, Lesley Forrester
    Article
    Petazzi et al Elife. 2024 Dec 23;13:RP94884. doi: 10.7
    Inserts
    UniSAM insert
    TET-ON
    Neo Resistance
    Insulator
    Use
    CRISPR and Synthetic Biology
    Tags
    T2A-mCherry Tag
    Expression
    Mammalian
    Promoter
    EF1a, NA, Splicing acceptor, and TREGV promoter
    Available Since
    Dec. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    211495-plasmid-map-sequence-id-454077

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  29. lentiMutB3GALT5catdead-blast

    Plasmid
    #220869
    Purpose
    lentiviral vector for expressing human B3GALT5 with D156A and D243A inactivating mutations and C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence
    Depositor
    Jennifer Kohler
    Article
    Ghorashi et al bioRxiv [Preprint]. 2023 Aug 3:2023.08.0
    Insert
    beta-1,3-galactosyltransferase 5 (B3GALT5 Human)
    Use
    Lentiviral
    Tags
    myc, FLAG
    Expression
    Mammalian
    Mutation
    encodes D156A and D243A mutations; also, nucleoti…
    Available Since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    220869-plasmid-map-sequence-id-440865

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Showing: 1981 - 2010 of 2018 results