We narrowed to 3,561 results for: luciferase expression
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Plasmid#124403PurposeGal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with T898A mutation.DepositorInsertGal4-TET1-878-911(T898A mutation) (TET1 Human)
UseTagsgal4 dna binding domainExpressionMammalianMutationT898APromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
Gal4-TET1-878-911-L900A(pL84)
Plasmid#124402PurposeGal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with L900A mutation.DepositorInsertGal4-TET1-878-911(L900A mutation) (TET1 Human)
UseTagsgal4 dna binding domainExpressionMammalianMutationL900APromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
Gal4-TET1-878-911-L897A(pL84)
Plasmid#124401PurposeGal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with L897A mutation.DepositorInsertGal4-TET1-878-911(L897A mutation) (TET1 Human)
UseTagsgal4 dna binding domainExpressionMammalianMutationL897APromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSi-check2-hRac2 3'UTR
Plasmid#97160PurposeFor dual luciferase report assayDepositorInsertRAC 3'UTR (AKT1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1A-MYC-IRES-lucOS
Plasmid#129776PurposeTransposon based vector that expresses human MYC followed by IRES and firefly luciferase and the antigens SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), and OVA323-339DepositorInserthuman MYC (MYC Human)
UseTagsOS: SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), a…ExpressionMammalianMutationPromoterEF1AAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL4-eIF3B MBE3 mutant
Plasmid#133310PurposeExpression of luciferase driven by eIF3B promoter region mutated at the E-box binding motif 3DepositorInserteIF3B promoter MBE3 mutant (EIF3B Human)
UseLuciferaseTagsExpressionMammalianMutationccacgtgacc changed to cAaAAAAaccPromotereIF3BAvailable sinceOct. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
BTV
Plasmid#131589PurposePlasmid for the targeting of BAC cloneDepositorInsertFirefly Luciferase
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
Luc-T20
Plasmid#177941PurposeTransient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-22222 (T20) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.DepositorInsertLuc2 (ORF1ab )
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRC49-U6-DR-crRNA-BsmbI(x2)-6T; EFS-Puro-2A-Fluc-2A-EGFP_NLS-WPRE
Plasmid#123363PurposeLentiviral vector with empty U6 cassette containing LbCpf1 direct repeat and U6 terminator, with constitutive expression of puromycin resistance, Firefly luciferase, and nuclear EGFP.DepositorInsertEGFP-NLS
UseCRISPR, Lentiviral, and LuciferaseTagsExpressionMammalianMutationPromoterEFSAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
p28-2iDP-puro
Plasmid#88893PurposeDual-intron DMD platform plasmid. Co-expresses DMD platform segment, firefly luciferase, puroR and mCherry. Plasmid carries phiC31 and Bxb1 attB sites.DepositorInsertsDual-intron DMD platform
luciferase
puromycin resistance enzyme
mCherry
UseTagsExpressionMammalianMutationTruncated version of the dystrophin protein (tran…PromoterAvailable sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
Luc-PS9
Plasmid#177942PurposeTransient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.DepositorInsertLuc2 (ORF1ab )
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCh-LgBiT-2A-EGFP-Vin-PILATeS-CT
Plasmid#216763PurposePiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS-CT (700 pM) negative control in mammalian cells. Requires transposaseDepositorInsertmCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS-CT
UseTagsEGFP and mCherryExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLD-CAG-DYSF-CFL
Plasmid#131457PurposeEncodes Dysferlin protein (Therapeutic gene) along with Follistatin protein to cure LGMD2B along with luciferase enzyme for in vivo live imagingDepositorUseTagsExpressionBacterial and MammalianMutationR15C (in DYSF) and Q2579H (in CFL)PromoterAvailable sinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
LUC2 (L/Sec/deltaP)
Plasmid#112145PurposeMammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activityDepositorInsertLuciferase coding region with Cysteine 258 mutated to selenocysteine (U) and deleted AUGA in PHGPx SECIS core (Gpx4 Photinus pyralis, Rat)
UseTagsExpressionMammalianMutationCysteine 258 mutated to selenocysteine (U) and d…PromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
LUC45 (L/Sec/mutP)
Plasmid#112148PurposeMammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activityDepositorInsertLuciferase coding region with Cysteine 258 mutated to selenocysteine (U) and MUTANT AUGA PHGPx SECIS core (Gpx4 Photinus pyralis, Rat)
UseTagsExpressionMammalianMutationCysteine 258 mutated to selenocysteine (U) and SE…PromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-his-Gluc-iLID
Plasmid#172096PurposeExpresses a fusion protein of Gluc and iLID, which dimerizes with a sspB-tagged protein when the bioluminescent reaction is initiated, in bacteriaDepositorInsertGaussia Luciferase - iLID
UseTagsExpressionBacterialMutationGluc Mutations - M43L, M110LPromoterT7Available sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGB SF-35S:Renilla:TNOS-35S:P19:TNOS (GB0160)
Plasmid#75412PurposeModule for the expression of the Renilla Luciferase with the silencing suppressor P19DepositorInsertRenilla / P19
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationPromoter35SAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Gluc-EL222-his
Plasmid#172097PurposeExpresses the fusion protein of Gluc and the light-activated transcription factor EL222 in bacteriaDepositorInsertGaussia Luciferase - EL222
UseTagsExpressionBacterialMutationGluc mutations - M43L, M110LPromoterT7Available sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1618 pAAV SYN1 Nuc-EYFP miR-30 FF3
Plasmid#135564PurposeAn AAV vector expressing miR-30a shRNA vs FF luciferase and a nuclear EYFP reporterDepositorInsertsmiR-30 FF3
Nuc-EYFP
UseAAVTags3xNLSExpressionMammalianMutationPromoterSYN1 and hSYN1Available sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMCSF-R(m40)-luc
Plasmid#12425DepositorInsertM-CSF receptor promoter fragment (CSF1R Human)
UseLuciferaseTagsExpressionMutationPU.1 binding site mutation (created a KpnI site i…PromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pFrt-invCAG-Luc
Plasmid#63577PurposeShuttle vector compatible with Flp-mediated (Flp-in) transgene integration that allows for conditional Luciferase expression upon integration. The vector contains an FRT site and two mutant loxP sitesDepositorInsertStop-Lox71-Luciferase-pA-FRT-Lox66-reverseCAG
UseCre/Lox; Frt/flpe recombination mediated insertionTagsExpressionMammalianMutationPromoterAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
CIP2Aprom27bp-pGL4.10Luc
Plasmid#60876PurposeLuc reporter driven by 27 bp of the CIP2A promoterDepositorInsert27 bp promoter fragment of Cancerous Inhibitor of PP2A (CIP2A Human)
UseLuciferase; PromoterlessTagsExpressionMutationPromoterAvailable sinceApril 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
CIP2Aprom1802bp-pGL4.10Luc
Plasmid#60868PurposeLuc reporter driven by 1802 bp of the CIP2A promoterDepositorInsert1802 bp promoter fragment of Cancerous Inhibitor of PP2A (CIP2A Human)
UseLuciferase; PromoterlessTagsExpressionMutationPromoterAvailable sinceApril 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
CIP2Aprom285bp-pGL4.10Luc
Plasmid#60873PurposeLuc reporter driven by 285 bp of the CIP2A promoterDepositorInsert285 bp promoter fragment of Cancerous Inhibitor of PP2A2 (CIP2A Human)
UseLuciferase; PromoterlessTagsExpressionMutationPromoterAvailable sinceApril 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
CIP2Aprom204bp-pGL4.10Luc
Plasmid#60874PurposeLuc reporter driven by 204 bp of the CIP2A promoterDepositorInsert204 bp promoter fragment of Cancerous Inhibitor of PP2A (CIP2A Human)
UseLuciferase; PromoterlessTagsExpressionMutationPromoterAvailable sinceApril 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
CIP2Aprom335bp-pGL4.10Luc
Plasmid#60872PurposeLuc reporter driven by 335 bp of the CIP2A promoterDepositorInsert335 bp promoter fragment of Cancerous Inhibitor of PP2A (CIP2A Human)
UseLuciferase; PromoterlessTagsExpressionMutationPromoterAvailable sinceApril 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
CIP2Aprom392bp-pGL4.10Luc
Plasmid#60871PurposeLuc reporter driven by 392 bp of the CIP2A promoterDepositorInsert392 bp promoter fragment of Cancerous Inhibitor of PP2A (CIP2A Human)
UseLuciferase; PromoterlessTagsExpressionMutationPromoterAvailable sinceApril 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
CIP2Aprom417bp-pGL4.10Luc
Plasmid#60870PurposeLuc reporter driven by 417 bp of the CIP2A promoterDepositorInsert417 bp promoter fragment of Cancerous Inhibitor of PP2A (CIP2A Human)
UseLuciferase; PromoterlessTagsExpressionMutationPromoterAvailable sinceApril 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
CIP2Aprom865bp-pGL4.10Luc
Plasmid#60869PurposeLuc reporter driven by 865 bp of the CIP2A promoterDepositorInsert865 bp promoter fragment of Cancerous Inhibitor of PP2A (CIP2A Human)
UseLuciferase; PromoterlessTagsExpressionMutationPromoterAvailable sinceApril 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-CD69 CaRE1 (minus strand)
Plasmid#91842PurposeLuciferase reporter for CD69 enhancer (IGI-P0619)DepositorInsertCD69 CaRE1 (minus strand) (CD69 Human)
UseLuciferaseTagsExpressionMammalianMutationPerfect match to reference sequencePromoterAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-CD69 CaRE2 (plus strand)
Plasmid#91848PurposeLuciferase reporter for CD69 enhancer (IGI-P0625)DepositorInsertCD69 CaRE2 (plus strand) (CD69 Human)
UseLuciferaseTagsExpressionMammalianMutationPerfect match to reference sequencePromoterAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-CD69 CaRE2 (minus strand)
Plasmid#91843PurposeLuciferase reporter for CD69 enhancer (IGI-P0620)DepositorInsertCD69 CaRE2 (minus strand) (CD69 Human)
UseLuciferaseTagsExpressionMammalianMutationPerfect match to reference sequencePromoterAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-Eef2TOP-RLCP-HCVIRES-FL
Plasmid#233628PurposeExpression of bicistronic reporter with TOP motifDepositorInsertPromoter and 5'UTR of Eef2 gene and HCV IRES (Eef2 Mouse)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-Eef2TOPm-RLCP-HCVIRES-FL
Plasmid#233629PurposeExpression of bicistronic reporter with mutated TOP motifDepositorInsertPromoter and 5'UTR of Eef2 gene with mutation in TOP motif and HCV IRES (Eef2 Mouse)
UseLuciferaseTagsExpressionMutationAGAAGG in TOP motifPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
ATG-2460
Plasmid#108713PurposeEF1-CBR2opt-T2A-copGFP (construct used for lentiviral transduction)DepositorInsertCBR2opt-copGFP fusion
UseLentiviralTagsCBR2opt fused to copGFPExpressionMutationPromega used directed evolution to create CBR (Cl…Promoterelongation factor-1Available sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo-RL-ZEB1
Plasmid#35535DepositorInsert3'UTR of ZEB1 (ZEB1 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo-RL-ZEB1 200bmutx5
Plasmid#35537DepositorInsert3'UTR of ZEB1 (ZEB1 Human, Budding Yeast)
UseLuciferaseTagsExpressionMammalianMutation5 mutations in miR200b seed regions of 3'UTR…PromoterAvailable sinceMay 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo-RL-ZEB2 200bmut
Plasmid#35538DepositorInsert3'UTR of ZEB2 gene (ZEB2 Human)
UseLuciferaseTagsExpressionMammalianMutation6 Mutations in the miR-200b seed regions of the Z…PromoterAvailable sinceJune 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pNOC_hfnCas12a_CRATES
Plasmid#176251PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and type IIS restriction site for endonuclease BaeI at the crRNA region that facilitates the generation of mulitplDepositorInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseExpressionMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
psicheck2 PTEN 3'UTR
Plasmid#50936Purposereporter for PTEN RNAiDepositorInsertPTEN 3'UTR (PTEN Human)
UseRNAiTagsRenilla luciferaseExpressionMammalianMutationPromoterT7Available sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only