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Showing: 3301 - 3350 of 37659 results

  1. pSMART-Mev1

    Plasmid
    #65815
    Purpose
    NADPH Dependent Mevalonic Acid Production Construct, Low Phosphate Dependent E. coli Expression
    Depositor
    Michael Lynch
    Inserts
    mvaE: bifunctional acetoacetyl-CoA thiolase, and NADPH dependent HMG-CoA reductase
    mvaS: hydroxymethylglutaryl-CoA synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    insulated mipAp from E. coli and insulated waaHp…
    Available since
    Sept. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    65815-plasmid-map-sequence-id-111947

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  3. pDGB3 alpha1 Tnos:nptII:Pnos - SF - P35S:hCas9:Tnos (GB1656)

    Plasmid
    #160618
    Purpose
    Module for the expression of the human codon optimized Cas9 and kanamycin resistance (NptII).
    Depositor
    Diego Orzaez
    Insert
    Tnos:NptII:Pnos-SF-P35s:hCas9:tNos
    Use
    CRISPR
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Pnos, 35S
    Available since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160618-plasmid-map-sequence-id-322016

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  7. pCB30

    Plasmid
    #111910
    Purpose
    Expresses gRNA to target URA3 knockout locus and has KanMX marker
    Depositor
    Yi Tang
    Insert
    gRNA cassette targeting URA3 knockout locus
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    SNR52p
    Available since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    111910-plasmid-map-sequence-id-218310

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  10. AA566

    Plasmid
    #231115
    Purpose
    Fragmid fragment: (guide cassette) ABE activity-based selection cloning
    Depositor
    David Root, John Doench
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    231115-plasmid-map-sequence-id-458370

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  11. AA568

    Plasmid
    #231117
    Purpose
    Fragmid fragment: (guide cassette) CBE activity-based selection cloning
    Depositor
    David Root, John Doench
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    231117-plasmid-map-sequence-id-458363

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  13. pIND4-RC-C2-MmXYRS

    Plasmid
    #222744
    Purpose
    Expresses 3 subunits of photosynthetic reaction center from R. sphaeroides and aaRS-tRNA pair for incorporating haloalkane crosslinker
    Depositor
    Steven Boxer
    Inserts
    puhA
    pufL
    pufM
    MmXYRS
    Mm Pyl tRNA
    Use
    Tags
    6xHis
    Expression
    Mutation
    Contains the following substitutions: V346A, W348…
    Promoter
    rrnB
    Available since
    Sept. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    222744-plasmid-map-sequence-id-444741

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  25. pHL 2146

    Plasmid
    #69789
    Purpose
    KanR with FRT sites + pT7::SgrS::T1 terminator + ColE
    Depositor
    Han Lim
    Article
    Shin et al Nucleic Acids Res. 2016 Apr 15. pii: gkw
    Insert
    SgrS
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 2, 2016
    Availability
    Academic Institutions and Nonprofits only

  30. pLKO.1-puro U6 sgRNA BfuAI large stuffer

    Plasmid
    #52628
    Purpose
    lentiviral U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites, improved cassette cloning efficiency
    Depositor
    Scot Wolfe
    Insert
    Kanamycin cassette
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    April 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    52628-plasmid-map-sequence-id-217983

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  33. pUK21

    Plasmid
    #49788
    Purpose
    E. coli cloning vector (KanR, high copy, blue/white selection, M13 IR)
    Depositor
    Joachim Messing
    Article
    Vieira et al Gene. 1991 Apr;100:189-94.
    Type
    Empty backbone
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    lacZ
    Available since
    July 10, 2014
    Availability
    Academic Institutions and Nonprofits only
    49788-plasmid-map-sequence-id-160334

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  35. pZ8-Ptac

    Plasmid
    #74064
    Purpose
    IPTG inducible version of pZ8-1 plasmid, to which lacIq was added, KanR
    Depositor
    Timothy Lu
    Article
    Cleto et al ACS Synth Biol. 2016 Feb 16.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    ptac
    Available since
    April 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    74064-plasmid-map-sequence-id-138074

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  43. pZ8-Prp

    Plasmid
    #74065
    Purpose
    pZ8-1 with the PprpD2 propionate-inducible promoter instead of Ptac, KanR
    Depositor
    Timothy Lu
    Article
    Cleto et al ACS Synth Biol. 2016 Feb 16.
    Insert
    Propionate inducible promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    prp
    Available since
    April 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    74065-plasmid-map-sequence-id-138071

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  45. pBTK1056

    Plasmid
    #209546
    Purpose
    BTK Type 4 antibiotic resistance cassette for Golden Gate assembly
    Depositor
    Jeffrey Barrick
    Article
    Lariviere et al bioRxiv 2023.09.19.558440
    Insert
    Type 4 Kanamycin resistance cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 12, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    209546-plasmid-map-sequence-id-419119

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Showing: 3301 - 3350 of 37659 results