We narrowed to 6,722 results for: tac
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Plasmid#77662Purpose3rd generation lentiviral gRNA plasmid targeting human SEPHS2DepositorInsertSEPHS2 (SEPHS2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCherry-miniSOG-Tubulin-6
Plasmid#55097PurposeLocalization: Microtubules, Excitation: 448 / 473, Emission: 500 / 528DepositorInsertTubulin (TUBA1B Human)
UseTagsmCherry-miniSOGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
SNRK gRNA (BRDN0001146508)
Plasmid#76732Purpose3rd generation lentiviral gRNA plasmid targeting human SNRKDepositorInsertSNRK (SNRK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
ROCK1 gRNA (BRDN0001147013)
Plasmid#77596Purpose3rd generation lentiviral gRNA plasmid targeting human ROCK1DepositorInsertROCK1 (ROCK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Flag-YAP-P129D
Plasmid#33084DepositorInsertYAP (YAP1 Human)
UseTagsFlagExpressionMammalianMutationP129DPromoterCMVAvailable sinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pX459-SOS1(dog)-gRNA2
Plasmid#228756PurposeA knockout vector for dog SOS1.DepositorInsertA gRNA targeting the dog SOS1 gene and the cDNA of CRISPR-Cas9 (SOS1 canis lupus)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPB-[EF1a-mRuby2(miRE.FF4)-TS.FF6x1-bGH]-UbC-PuroR-T2A-mGL-SV40pA
Plasmid#235326PurposePiggyBac integration vector for ComMAND open-loop circuit regulating mRuby2DepositorInsertmRuby2
UseSynthetic Biology; PiggybacTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiX1-[TRE3G-mRuby2-miRE.FF4-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE
Plasmid#235318PurposeLentiviral expression of mRuby2 with 3' microRNA onlyDepositorInsertmRuby2
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G (3rd-generation Tet system)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPB-[EF1a-mRuby2(miRE.FF4)-TS.FF4x1-bGH]-UbC-PuroR-T2A-mGL-SV40pA
Plasmid#235327PurposePiggyBac integration vector for ComMAND closed-loop circuit regulating mRuby2DepositorInsertmRuby2
UseSynthetic Biology; PiggybacTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiX1-[TRE3G-mRuby2-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE
Plasmid#235268PurposeLentiviral expression of ComMAND base gene regulating mRuby2DepositorInsertmRuby2
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G (3rd-generation Tet system)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiX1-[TRE3G-mRuby2(miRE.FF4)-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE
Plasmid#235317PurposeLentiviral expression of mRuby2 with intronic microRNA onlyDepositorInsertmRuby2
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G (3rd-generation Tet system)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pk335-CARGO-6mer-30kb-DSF
Plasmid#227483Purpose6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locusDepositorInsertCARGO to 30kb Downstream Fgf5
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pk335-CARGO-5mer-30kb-DSF
Plasmid#227484Purpose5-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locusDepositorInsertCARGO to 30kb Downstream Fgf5
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pk335-CARGO-4mer-30kb-DSF
Plasmid#227485Purpose4-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locusDepositorInsertCARGO to 30kb Downstream Fgf5
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pk335-CARGO-6mer-35kb-DSF-1-6
Plasmid#227487Purpose6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locusDepositorInsertCARGO to 35kb Downstream Fgf5
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pk335-CARGO-6mer-26kb-USF
Plasmid#227467Purpose6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locusDepositorInsertCARGO to 26kb Upstream Fgf5
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pk335-CARGO-6mer-14kb-USF-7-12
Plasmid#227471Purpose6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locusDepositorInsertCARGO to 14kb Upstream Fgf5
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pk335-CARGO-6mer-4.5kb-USP
Plasmid#227449Purpose6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locusDepositorInsertCARGO to 4.5kb Upstream Prdm8
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
OMM-short-CFAST10
Plasmid#233597PurposeExpression of CFAST10 on the OMM membrane after a short linkerDepositorInsertCFAST10
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
ER-RspA-CFAST
Plasmid#233607PurposeExpression of RspA-CFAST on the ER membraneDepositorInsertRspA-CFAST
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
shRNA_CTNNB1
Plasmid#215219PurposeSupression of shCTNNB1 expressionDepositorInsertCTNNB1 shRNA (CTNNB1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLViP-LifeAct-TagRFPT
Plasmid#229700PurposeLentiviral expression of LifeAct-TagRFPT in mammalian cellsDepositorInsertSynthetic construct LifeAct-mTurquoise gene
UseLentiviralTagsTagRFPTExpressionMammalianMutationPromoterCMV promoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-MYL2-HA-TadA8e-Sauri-U6-Camk2d sgRNA7
Plasmid#220127PurposeExpresses SauriABE8e by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter, and add an HA tag fused to TadADepositorInsertMYL2, TadA, nSauriCas9
UseAAVTagsHA tagExpressionMutationPromoterMYL2Available sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-MYL2-inteinC-Sauri C aa439-1061-U6-Camk2d sgRNA7
Plasmid#220129PurposeExpresses Sauri cas9C by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoterDepositorInsertMYL2, inteinC, nSauri Cas9C
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterMYL2Available sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCHC025 (∆CBBp)
Plasmid#226203PurposeElectroporation knockout plasmid (pK18sB) to delete the C. necator megaplasmid copy of the CBB operon (deltaCBBp).DepositorInsertHomology arms for megaplasmid copy of the CBB operonthe CBB operon (deltaCBBp).
UseSynthetic BiologyTagsExpressionMutation∆cbbR ∆cbbLpSpXpYpEpFpPpTpZpGpKpApPromoterAvailable sinceNov. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
p40651-EFSNS-FMR1_e1ΔCGG-24xms2
Plasmid#222969PurposeMS2 mRNA reporter with FMR1 5' UTR (CGG repeats removed) and exon 1 sequenceDepositorInsertFMR1-ΔCGG 5' UTR and exon1
UseTagsExpressionMammalianMutation31 CGG repeats deleted from 5' UTRPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-FKBP-LBR
Plasmid#222427PurposeTo express LBR with N-terminal GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cellsDepositorInsertLBR (LBR Human)
UseTagsGFP-FKBPExpressionMammalianMutationPromoterAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO mTagBFP2-GABARAP
Plasmid#212110PurposeExpression of N-terminally tagged GABARAP with mTagBFP2DepositorInsertmTagBFP2-GABARAP (GABARAP Entacmaea quadricolor, Human)
UseTagsmTagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
Amber-free HIV-1 deltaRT
Plasmid#213007PurposeAn replication-incompetent amber-free HIV-1 encodes full-length Env BG505 with RT-deleted Q23 as the backbone.DepositorInsertsHIV-1 Q23 ΔRT backbone (reverse transcriptase is deleted)
Env BG505
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCALPS-FLAG-HA-mmuMettl3-LN
Plasmid#207129PurposeLentiviral plasmid to express mouse Mettl3 Lambda peptide C-terDepositorInsertMettl3 (Mettl3 Mouse)
UseLentiviralTagsFLAG-HA and Lambda Peptide (LN)ExpressionMutationPromoterSSFVAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUBQ10:LYK5_At-3xHA
Plasmid#202192PurposeExpress Arabidopsis thaliana LYK5 gene under UBQ10 promoterDepositorInsertLYSM-CONTAINING RECEPTOR-LIKE KINASE 5 (LYK5 Mustard Weed)
UseTagsHAExpressionPlantMutationPromoterAtUBQ10Available sinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2/shCol1a1/Scarlet_Seq1.2
Plasmid#201404PurposeKnockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertCOL1A1
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2/shCol1a1/Scarlet-Seq1.1
Plasmid#201401PurposeKnockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertCOL1A1
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2/Col1a1/GFP4_Seq 1.1
Plasmid#201400PurposeKnockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertCOL1A1
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
p426-Cup1p-FusionRed
Plasmid#188392PurposeCu2+-dependent expression of red fluorescent protein in yeast cellsDepositorInsertFusionRed
UseTagsExpressionYeastMutationPromoterCUP1 promoterAvailable sinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcmv3-IRG1-H277Y-HA
Plasmid#198185PurposeMammalian expression of HA-tagged human ACOD1 (IRG1) H277YDepositorInsertACOD1 (ACOD1 Human)
UseTagsHAExpressionMammalianMutationThe histidine in IRG1 at position 277 mutates to …PromoterCMVAvailable sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003 sgINTS12 guide 1
Plasmid#193606PurposeINTS12 knockoutDepositorInsertsgINTS12 guide 1 (INTS12 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPlacPbuCas13b-gRNA-NT
Plasmid#184838PurposeExpression of single-spacer CRISPR array with a shortened non-targeting spacer for PbuCas13b and expression of PbuCas13b in bacteria.DepositorInsertCo-expression of single-spacer CRISPR array with a nontargeting spacer for PbuCas13b and PbuCas13b nuclease in bacteria.
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119 and lac promoterAvailable sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-Duet1-6xHis-TEV-OPTN
Plasmid#190192PurposePlasmid for the expression of 6xHis-TEV-OPTN. Internal reference: SMC397DepositorInsertoptineurin (OPTN Human)
UseTags6xHis and TEVExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET15b MOSPD2 [282-490]
Plasmid#186469PurposeExpression of human MOSPD2 (MSP domain from residue 282 to 490), fused to a 6HIS tag, in bacteriaDepositorInsertMOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
UseTags6HISExpressionBacterialMutationMSP domain [282-490]PromoterAvailable sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.U6gT28.4.hSyn.flex.H2B.RFP
Plasmid#170386PurposePlasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 4. Cre-inducible expression of nuclear RFP.DepositorInsertCre inducible H2B RFP
UseAAVTagsExpressionMutationPromoterhuman SynapsinAvailable sinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.U6gT28.3.hSyn.flex.H2B.RFP
Plasmid#170374PurposePlasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 3. Cre-inducible expression of nuclear RFP.DepositorInsertCre inducible H2B RFP
UseAAVTagsExpressionMutationPromoterhuman SynapsinAvailable sinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-U6-UL29-U3-UL8
Plasmid#166685PurposeTo express gRNA expression cassette simultaneously targeting two genes: UL8 and UL29 (non-EGFP version).DepositorUseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1B CDC25C_2
Plasmid#160770PurposeSuppress CDC25CDepositorInsertshCDC25C_2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1B CDC25C_1
Plasmid#160769PurposeSuppress CDC25CDepositorInsertshCDC25C_1
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLCHKO_HPRT1_exon2_1_As
Plasmid#155055PurposeLentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and AsCas12a nucleases (CHyMErA system)DepositorInsert(hg)RNA for deletion of HPRT1 exon 2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
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