We narrowed to 6,905 results for: crispr cas9 plasmids

1. pSpCas9-2A-Puro-RAB7A-gRNA2 (PX459)

   Plasmid

   #221552

   Purpose

   Expresses Cas9 and gRNA for disruption of Rab7A gene in human cells

   Depositor

   Shawn Ferguson

   Article

   Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

   Insert

   gRNA targeting human Rab7A (RAB7A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX601-AAV-CMV::NLS-saCas9-NLS-3xHA-bGHpa;U6::BsaI-sgRNA-lacZ

   Plasmid

   #175175

   Purpose

   A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting lacZ

   Depositor

   Bernardo Sabatini

   Article

   Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25.

   Insert

   sgRNA-lacZ
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX601-AAV-CMV::NLS-saCas9-NLS-3xHA-bGHpa;U6::BsaI-sgRNA-Grpr1

   Plasmid

   #175176

   Purpose

   A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Grpr

   Depositor

   Bernardo Sabatini

   Article

   Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25.

   Insert

   sgRNA-Grpr
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNOC-CRISPR-35S

   Plasmid

   #99861

   Purpose

   Expresses Cas9-Nlux and sgRNA. Contains CEN/ARS and 35 terminator. No selection marker.

   Depositor

   Eva Farre

   Article

   Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

   Type

   Empty backbone

   Use

   Algae, nannochloropsis expression

   Tags

   Cas9-Nlux

   Expression

   Mutation

   Promoter

   Ribi promoter

   Available since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNOC-CRISPR-compact

   Plasmid

   #99369

   Purpose

   Expresses Cas9-Nlux and sgRNA. Contains CEN/ARS. No selection marker.

   Depositor

   Eva Farre

   Article

   Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

   Type

   Empty backbone

   Use

   Algae, nannochloropsis expression

   Tags

   Cas9-Nlux

   Expression

   Mutation

   Promoter

   Ribi promoter

   Available since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. L40C-CRISPR.EFS.mNeon

   Plasmid

   #69146

   Purpose

   Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA (hU6), mNeon coexpression, EFS Promoter driven

   Depositor

   Dirk Heckl

   Article

   Reimer et al Haematologica. 2017 Jun 1. pii: haematol

   Inserts

   SpCas9
   Sp sgRNA scaffold
   EFS (EEF1A1 Human)
   P2A-mNeonGreen
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBK2047-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA2)

   Plasmid

   #223167

   Purpose

   2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and gRNA2 targeting human/mouse APOE

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   KRAB-MECP2 (MECP2 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 204-310 (MECP2)

   Promoter

   EF1a

   Available since

   Aug. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBK2046-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA1)

   Plasmid

   #223166

   Purpose

   2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and gRNA1 targeting human/mouse APOE

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   KRAB-MECP2 (MECP2 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 204-310 (MECP2)

   Promoter

   EF1a

   Available since

   Aug. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRISPR-HOT_tdTomato

   Plasmid

   #138567

   Purpose

   Universal Cleavable Donor Plasmid for tagging with tdTomato using NHEJ

   Depositor

   Hans Clevers

   Article

   Artegiani et al Nat Cell Biol. 2020 Mar;22(3):321-331. d

   Insert

   tdTomato
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. (565) pAAV Alb-AAT KRAB-SadCas9 U6-gSTOP

    Plasmid

    #163030

    Purpose

    Expression of CRISPRi with gSTOP gRNA

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Mammalian codon-optimized SadCas9
    

    Use

    AAV

    Tags

    KRAB, SV40 pA, and myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Alb-AAT

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. eSpCas9-hGeminin

    Plasmid

    #199344

    Purpose

    modified version of the eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA plasmid (addgene #86613) where the C-terminus of the eSpCas9 enzyme was fused to amino acids 1 – 110 of human Geminin

    Depositor

    Iain Hagan

    Article

    Morrison et al Open Biol. 2023 Apr;13(4):230021. doi: 1

    Insert

    ATP1A1 G3 sgRNA+user-specified sgRNA+enhanced specificity Cas9 (1.1) (addgene 86613) with cas9 fused to hgem fragment 1-110) (GMNN S. pyogenes)
    

    Use

    Tags

    cas9 c-term fused to hgemenin 1-110 fragment

    Expression

    Mammalian

    Mutation

    cas9 c-term fused to hgemenin 1-110 fragment

    Promoter

    cbh

    Available since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. JI501: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 gRNA scaffold

    Plasmid

    #121841

    Purpose

    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; empty hU6-driven SaCas9 gRNA scaffold for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to dCas9 w/ gRNA expression

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. LB001: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::Sp/xCas9 TRE#2 gRNA

    Plasmid

    #131757

    Purpose

    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven Sp/xCas9 TRE#2 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#2 gRNA expression

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    TRE#2 Sp/xCas9 gRNA
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Oct. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. LA901: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::Sp/xCas9 TRE#1 gRNA

    Plasmid

    #131756

    Purpose

    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven Sp/xCas9 TRE#1 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#1 gRNA expression

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    TRE#1 Sp/xCas9 gRNA
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Oct. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. JV201: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 TRE#1 gRNA

    Plasmid

    #131755

    Purpose

    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven SaCas9 TRE#1 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#1 gRNA expression

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    TRE#1 SaCas9 gRNA
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Oct. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LB101: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 TRE#2 gRNA

    Plasmid

    #131758

    Purpose

    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven SaCas9 TRE#2 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#2 gRNA expression

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    TRE#2 SaCas9 gRNA
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. L-CRISPR-CTN (MLL-ENL)

    Plasmid

    #69212

    Purpose

    Lentiviral CRISPR-Cas9 vector for induction of chromosomal translocations; MLL-ENL,(t[11;19])

    Depositor

    Dirk Heckl

    Article

    Reimer et al Haematologica. 2017 Jun 1. pii: haematol

    Inserts

    SpCas9
    Sp sgRNA scaffold
    EFS (EEF1A1 Human)
    P2A-mNeonGreen
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNOC-CRISPR-P2A-BlastR

    Plasmid

    #98147

    Purpose

    Expresses Cas9-Nlux-P2A-BlastR and sgRNA in Nannochloropsis oceanica.

    Depositor

    Eva Farre

    Article

    Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

    Type

    Empty backbone

    Use

    Algae, nannochloropsis expression

    Tags

    P2A-BlastR fusion with Cas9-Nlux

    Expression

    Mutation

    Promoter

    Ribi promoter

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. CAG-LoxpStopLoxp-NLS-dCas9-HA-NLS-NLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-T2A-EGFP-WPRE-PolyA

    Plasmid

    #107307

    Purpose

    Encoding an SPH activation system

    Depositor

    Hui Yang

    Article

    Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

    Insert

    CAG-LSL-dCas9-10xGCN4-P2A-scFv-P65-HSF1-T2A-EGFP-WPRE-PolyA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. LentiCRISPRv2-sgCTRL

    Plasmid

    #209750

    Purpose

    Lentiviral transfer plasmid to express Cas9 and a control, non-specific gRNA (does not target any human gene)

    Depositor

    Andrei Thomas-Tikhonenko

    Article

    Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

    Insert

    Cas9
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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