We narrowed to 1,704 results for: pyogenes Cas9

1. pSLQ1869-1 pHR: U6-SpsgSV40 CMV-mCherry

   Plasmid

   #84249

   Purpose

   Expresses Sp sgSV40 gRNA with a mCherry fluorescent marker

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Insert

   Sp sgSV40
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDA010.188

   Plasmid

   #206792

   Purpose

   J23113.dCas9

   Depositor

   James Carothers

   Article

   Alba Burbano et al Proc Natl Acad Sci U S A. 2023 Jul 25;12

   Insert

   dCas9
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   J23113

   Available since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCK389

   Plasmid

   #206791

   Purpose

   Sp.dCas9, pTet.MCP-SoxS, J23119.gRNA

   Depositor

   James Carothers

   Article

   Alba Burbano et al Proc Natl Acad Sci U S A. 2023 Jul 25;12

   Inserts

   dCas9
   MCP-SoxS
   scRNA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   J23119 and Tet

   Available since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEPOR0SP0019

   Plasmid

   #117529

   Purpose

   Level0 Golden Gate part: CDS, Sp-eCas9 1.1 (no stop codon)

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   Sp-eCas9 1.1 (no stop codon)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   K848A, K1003A, R1060A

   Promoter

   Available since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEPOR0SP0017

   Plasmid

   #117527

   Purpose

   Level0 Golden Gate part: CDS, Sp-eCas9 1.0 (no stop codon)

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   Sp-eCas9 1.0 (no stop codon)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   K810A, K1003A, R1060A

   Promoter

   Available since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEPOR0SP0011

   Plasmid

   #117530

   Purpose

   Level0 Golden Gate part: CDS, Sp-xCas9 3.7 (no stop codon)

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   Sp-xCas9 3.7 (no stop codon)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   A262T, R324L, S409I, E480K, E543D, M694I, E1219V

   Promoter

   Available since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCT310

   Plasmid

   #111140

   Purpose

   Bacterial expression of 6His-dCas9-3XFlag

   Depositor

   Robert Tjian

   Article

   Tsui et al Proc Natl Acad Sci U S A. 2018 Mar 20;11

   Insert

   dCas9
   

   Use

   Tags

   3X Flag and 6X His

   Expression

   Bacterial

   Mutation

   D10A - H840A

   Promoter

   T7

   Available since

   July 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pS648∙CsR

   Plasmid

   #197860

   Purpose

   Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

   Depositor

   Susanne Häußler

   Article

   Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

   Insert

   gRNA scaffold - Gm resistance cassette - incP oriT
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   PEM7

   Available since

   Oct. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pS548∙CsR

   Plasmid

   #197859

   Purpose

   Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

   Depositor

   Susanne Häußler

   Article

   Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

   Insert

   gRNA scaffold - Tc resistance cassette - incP oriT
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   PEM7

   Available since

   Oct. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pS848∙CsR

    Plasmid

    #199240

    Purpose

    Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

    Depositor

    Susanne Häußler

    Article

    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

    Insert

    gRNA scaffold - Gm resistance cassette - incP oriT
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PEM7

    Available since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. MSP469

    Plasmid

    #65771

    Purpose

    Human expression vector for SpCas9 VQR variant: CMV-T7-humanSpCas9(D1135V/R1335Q/T1337R)-NLS-3xFLAG (VQR variant)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/R1335Q/T1337R)-NLS-3XFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    D1135V, R1335Q, and T1337R mutations in Cas9

    Promoter

    CMV & T7

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSP680

    Plasmid

    #65772

    Purpose

    Human expression vector for SpCas9 EQR variant: CMV-T7-humanSpCas9(D1135E/R1335Q/T1337R)-NLS-3xFLAG (EQR variant)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E/R1335Q/T1337R)-NLS-3XFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    D1135E, R1335Q, and T1337R mutations in Cas9

    Promoter

    CMV & T7

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. miniCGBE1 (pBM1219)

    Plasmid

    #140253

    Purpose

    CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    BE4max(R33A)-ΔUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, D10A in Cas9

    Promoter

    CMV

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1_pCMV-nCas-PmCDA1-ugi pH1-gRNA(HPRT)

    Plasmid

    #79620

    Purpose

    Expresses nCas9-PmCDA1-UGI and gRNA(HPRT) in mammalian cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Use

    Tags

    PmCDA1 and UGI

    Expression

    Mammalian

    Mutation

    D10A for nickase Cas9

    Promoter

    pCMV

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRDA_831

    Plasmid

    #216100

    Purpose

    CRISPRa, EF1a-driven dCas9-VP64 (Cas only)

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    Cas9 [Sp]
    

    Use

    CRISPR and Lentiviral; Assembled vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. iPEmax-D10A

    Plasmid

    #239386

    Purpose

    For inverse prime editor using nCas9-D10A-R221K-N394K in HEK295T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Commun. 2025 May 31;16(1):5057. doi:

    Insert

    nCas9-D10A-R221K-N394K, M-MLV-RT
    

    Use

    CRISPR

    Tags

    BPNLS and SV40 NLS, c-myc NLS

    Expression

    Mammalian

    Mutation

    D10A, R221K, N394K in Cas9

    Promoter

    CMV

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJT175_GalL_A3A(Y130F)Δ186-BE3

    Plasmid

    #145124

    Purpose

    Expressing base editor A3A(Y130F)Δ186-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    A3A(Y130F)Δ186-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    A3A(Y130F; 1-186AA); spCas9(D10A)

    Promoter

    GalL

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSLQ1852-2 pHR: U6-SpsgCD95-1 CMV-EGFP

    Plasmid

    #84251

    Purpose

    Expresses Sp sgCD95-1 gRNA with an EGFP fluorescent marker

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Insert

    Sp sgCD95-1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSLQ1852-5 pHR: U6-SpsgCD95-3 CMV-EGFP

    Plasmid

    #84253

    Purpose

    Expresses Sp sgCD95-3 gRNA with an EGFP fluorescent marker

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Insert

    Sp sgCD95-3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSLQ1852-3 pHR: U6-SpsgCD95-2 CMV-EGFP

    Plasmid

    #84252

    Purpose

    Expresses Sp sgCD95-2 gRNA with an EGFP fluorescent marker

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Insert

    Sp sgCD95-2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge