We narrowed to 1,621 results for: sp1

1. pPB-cT3G-cERP2-3xFLAG-SMAD1-active

   Plasmid

   #222579

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of 3xFLAG-SMAD1

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2024.05.31.596483

   Insert

   SMAD1 (SMAD1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   S487E, S489E

   Promoter

   Available since

   Aug. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSCV-IRES-GFP-Mouse-caspase-1/11a

   Plasmid

   #183391

   Purpose

   Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   CARD domain of mouse caspase-1 fused to catalytic domain of mouse caspase-11 (Casp1 Mouse, Synthetic)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mutation

   Promoter

   MESV

   Available since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMSCV-IRES-GFP-Mouse-caspase-1/11b

   Plasmid

   #183392

   Purpose

   Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   CARD domain of mouse caspase-1 fused to mouse caspase-11 (Casp1 Mouse, Synthetic)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mutation

   Promoter

   MESV

   Available since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGREAT16

   Plasmid

   #170904

   Purpose

   GoldenBraid Double Transcriptional Unit - pNOS_E-Luc_AtHSP18.2t+pNOS_Red-F_NOSt

   Depositor

   Markita Landry

   Article

   Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

   Insert

   E-Luc
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   c.375C>T silent mutation to remove BbsI site (…

   Promoter

   pNOS

   Available since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGREAT19

   Plasmid

   #170907

   Purpose

   GoldenBraid Double Transcriptional Unit - pG10-90_E-Luc_AtHSP18.2t+pNOS_Red-F_NOSt

   Depositor

   Markita Landry

   Article

   Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

   Insert

   E-Luc
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   c.375C>T silent mutation to remove BbsI site (…

   Promoter

   pG10-90

   Available since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGREAT20

   Plasmid

   #170908

   Purpose

   GoldenBraid Double Transcriptional Unit - pCmYLCV911_E-Luc_AtHSP18.2t+pNOS_Red-F_NOSt

   Depositor

   Markita Landry

   Article

   Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

   Insert

   E-Luc
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   c.375C>T silent mutation to remove BbsI site (…

   Promoter

   pCmYLCV911

   Available since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGREAT27

   Plasmid

   #170915

   Purpose

   GoldenBraid Double Transcriptional Unit - pAtNR1_E-Luc_AtHSP18.2t+pNOS_Red-F_NOSt

   Depositor

   Markita Landry

   Article

   Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

   Insert

   E-Luc
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   c.375C>T silent mutation to remove BbsI site (…

   Promoter

   pAtNR1

   Available since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Flag-M4-AR-W741C-S81A-P82A

   Plasmid

   #171250

   Purpose

   Mammalian expression of 3xFlag-tagged human androgen receptor Serine-Proline mutant that binds to bicalutamide: AR-Trp741Cys-Ser81Ala-Pro82Ala (AR-W741C-S81A-P82A)

   Depositor

   Steven Balk

   Insert

   3xFlag-tagged human androgen receptor Trp741Cys-Ser81Ala-Pro82Ala mutant (AR Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   AR-Trp741Cys-Ser81Ala-Pro82Ala (AR-W741C-S81A-P82…

   Promoter

   CMV

   Available since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Flag-M4-AR-W741C-S81A-P82G

   Plasmid

   #171251

   Purpose

   Mammalian expression of 3xFlag-tagged human androgen receptor Serine-Proline mutant that binds to bicalutamide: AR-Trp741Cys-Ser81Ala-Pro82Gly (AR-W741C-S81A-P82G)

   Depositor

   Steven Balk

   Insert

   3xFlag-tagged human androgen receptor Trp741Cys-Ser81Ala-Pro82Gly mutant (AR Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   AR-Trp741Cys-Ser81Ala-Pro82Gly (AR-W741C-S81A-P82…

   Promoter

   CMV

   Available since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA3-HA PSPC1 Y523F

    Plasmid

    #124373

    Purpose

    Expresses PSPC1 Y523F in mammalian cells

    Depositor

    Yuh-Shan Jou

    Article

    Lang et al Nat Commun. 2019 Dec 16;10(1):5716. doi:

    Insert

    PSPC1 (PSPC1 Human)
    

    Use

    Tags

    HA and HA tag

    Expression

    Mammalian

    Mutation

    Y523F

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMSCV-IRES-GFP-Caspase-1 (C285A) -NpLxIS

    Plasmid

    #131354

    Purpose

    Expresses Caspase-1 C285A with 2 copies of pLxIS motif from STING fused to its N-ternimus

    Depositor

    Jonathan Kagan

    Article

    Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

    Insert

    Caspase-1 (C285A)-NpLxIS (Casp1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Caspase-1 (C285A)

    Promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. GST-AR-1-36

    Plasmid

    #104192

    Purpose

    Expresses human AR aa 1-36 in pGEX-5x-1 bact expression vector with GST

    Depositor

    Elizabeth Wilson

    Article

    He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

    Insert

    human androgen receptor aa 1-36 (AR Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHAGE2 mCherry-Atl1

    Plasmid

    #86678

    Purpose

    Lentivirus to stably express fluorescent protein in mammalian cells

    Depositor

    Tom Rapoport

    Article

    Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

    Insert

    ATL1 (ATL1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. THBS1-bio-His

    Plasmid

    #53417

    Purpose

    Expresses full-length Thrombospondin-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    THBS1 (THBS1 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-hAR

    Plasmid

    #89078

    Purpose

    mammalian expression of human androgen receptor in pCMV5

    Depositor

    Elizabeth Wilson

    Article

    Lubahn et al Science. 1988 Apr 15;240(4850):327-30.

    Insert

    human androgen receptor (hAR) full-length (AR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLVX-opto-hCaspase-1

    Plasmid

    #208790

    Purpose

    Inducibly expresses human Caspase1, fused to light-activatable Cry2

    Depositor

    Petr Broz

    Article

    Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    Caspase1 (CASP1 Human)
    

    Use

    Lentiviral

    Tags

    Cry2 mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUltra-sY

    Plasmid

    #82417

    Purpose

    Expresses Mj aaRS for sulfotyrosine and Mj tRNA for decoding UAG codons

    Depositor

    Chang Liu

    Article

    Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542

    Insert

    Methanococcus jannaschii aaRS for sulfotyrosine
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Tyr32Leu, Leu65Pro, Asp158Gly, Ile159Cys, Leu162L…

    Promoter

    tacI

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRK5-FLAG-p14

    Plasmid

    #42330

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

    Insert

    p14 (LAMTOR2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCB-1

    Plasmid

    #210387

    Purpose

    All-in-one cumate-based inducible CRISPRi plasmid for use in Streptomyces

    Depositor

    Gilles van Wezel

    Article

    Bai et al ACS Synth Biol. 2023 Oct 6. doi: 10.1021

    Inserts

    dCas9
    CymR
    sgRNA cassette
    

    Use

    CRISPR; Integrative vector for use in streptomyces

    Tags

    Expression

    Mutation

    BsaI-flanked RFP cassette inserted in place of sp…

    Promoter

    SP11 and SP30

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCB-2

    Plasmid

    #210388

    Purpose

    All-in-one cumate-based inducible CRISPRi plasmid for use in Streptomyces

    Depositor

    Gilles van Wezel

    Article

    Bai et al ACS Synth Biol. 2023 Oct 6. doi: 10.1021

    Inserts

    dCas9
    CymR
    sgRNA cassette
    

    Use

    CRISPR; Integrative vector for use in streptomyces

    Tags

    Expression

    Mutation

    BsaI-flanked RFP cassette inserted in place of sp…

    Promoter

    SP11 and SP30

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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