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We narrowed to 4,284 results for: TIL

Showing: 3351 - 3400 of 4284 results

  3. MCP-MSN

    Plasmid
    #210704
    Purpose
    This plasmid express EF1a promoter driven MCP-MSN transactivation module
    Depositor
    Isaac Hilton
    Article
    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4
    Insert
    MCP fused to transactivation domain from Human transcription factor MRTF-A, STAT-1 and NRF2
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N55K in MCP
    Promoter
    EF1a
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    210704-plasmid-map-sequence-id-416247

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  6. pDG462

    Plasmid
    #100903
    Purpose
    SpCas9n (D10A Nickase mutant) with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A mutant converts to Nickase
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100903-plasmid-map-sequence-id-196607

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  9. MCP-eN3x9

    Plasmid
    #210706
    Purpose
    This plasmid express EF1a promoter driven MCP-eN3x9 transactivation module
    Depositor
    Isaac Hilton
    Article
    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4
    Insert
    MCP fused to transactivation domain comprising eNRF2 and 3x 9aa Transactivation domain
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N55K in MCP
    Promoter
    EF1a
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    210706-plasmid-map-sequence-id-416266

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  12. pCAG-hPPP3CA-FLAG

    Plasmid
    #179134
    Purpose
    Expression vector of human protein phosphatase 3, catalytic subunit, alpha isoform (PPP3CA) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.
    Depositor
    Masahito Ikawa
    Article
    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11
    Insert
    protein phosphatase 3, catalytic subunit, alpha isoform (PPP3CA Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179134-plasmid-map-sequence-id-352802

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  13. pET-TRSter

    Plasmid
    #78865
    Purpose
    Expresses rat Thioredoxin Reductase using an open reading frame in fusion with a mutated E. coli-type SECIS element
    Depositor
    Elias Arnér
    Article
    Arner et al J Mol Biol. 1999 Oct 8;292(5):1003-16.
    Insert
    Thioredoxin Reductase (Txnrd1 Rat)
    Use
    Tags
    Mutated E. coli-type SECIS element (3’ end of ins…
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac
    Available since
    Sept. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    78865-plasmid-map-sequence-id-147493

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  16. pTriEx-PA-Rac1

    Plasmid
    #22024
    Purpose
    Expression of photoactivatable Rac1
    Depositor
    Klaus Hahn
    Article
    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
    Insert
    PA-Rac1 (RAC1 Avena sativa (oat), Human)
    Use
    Tags
    6X His
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Rac1 starts at I4 and contains mutations Q61L, E9…
    Promoter
    CMV, p10
    Available since
    Sept. 29, 2009
    Availability
    Academic Institutions and Nonprofits only

  19. MCP-NMS

    Plasmid
    #210705
    Purpose
    This plasmid express EF1a promoter driven MCP-NMS transactivation module
    Depositor
    Isaac Hilton
    Article
    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4
    Insert
    MCP fused to transactivation domain from Human transcription factor NRF2, MRTF-A and STAT1
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N55K in MCP
    Promoter
    EF1a
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    210705-plasmid-map-sequence-id-416249

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  20. LV-indLS2

    Plasmid
    #123068
    Purpose
    Lentiviral construct that delivers a tamoxifen inducible Cre. Upon recombination, luc2 and mStrawberry express. Used to image spontaneous tumorigenesis or subclonal cell populations in vivo.
    Depositor
    Kevin Brindle
    Article
    Rodriguez et al J Pathol. 2014 Mar;232(4):449-57. doi: 1
    Inserts
    CreERT2 with intron
    Firefly luciferase
    mStrawberry
    Use
    Lentiviral and Luciferase; Fluorescence
    Tags
    Expression
    Mammalian
    Mutation
    synthetic intron added as indicated
    Promoter
    CAGGS (after Cre mediated inversion) and CAGGS (f…
    Available since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    123068-plasmid-map-sequence-id-240523

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  24. pTriEx-mVenus-PA-Rac1

    Plasmid
    #22007
    Purpose
    Expression of photoactivatable Rac1
    Depositor
    Klaus Hahn
    Article
    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
    Insert
    PA-Rac1 (RAC1 Avena sativa (oat), Human)
    Use
    Tags
    6X His and mVenus
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    LOV(Leu404-Leu546), Rac1 starts at I4 and contain…
    Promoter
    CMV, p10
    Available since
    Sept. 29, 2009
    Availability
    Academic Institutions and Nonprofits only

  25. pKK-TEV-CyOFP1

    Plasmid
    #105799
    Purpose
    Expression of your protein of interest in fusion with orange fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 27240196). CyOFP1 is useful for multi-channel imaging.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-CyOFP1
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105799-plasmid-map-sequence-id-203392

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  27. pGEX:PGRMC1

    Plasmid
    #136062
    Purpose
    Bacterial expression of pGEX:PGRMC1 to perform a GST-pulldown assay and identify new proteins that interact with GST-PGRMC1 in the human endometrial stroll cells.
    Depositor
    Francisco Domínguez Hernández
    Article
    Salsano et al Fertil Steril. 2020 May;113(5):1050-1066
    Insert
    Progesterone receptor membrane component 1 (PGRMC1 Human)
    Use
    Tags
    GST-PGRMC1
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    136062-plasmid-map-sequence-id-272028

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  33. pKK-CyOFP1-TEV

    Plasmid
    #105782
    Purpose
    Expression of your protein of interest in fusion with orange fluorescent protein at the N-terminus (cleavable by TEV) (PMID: 27240196). CyOFP1 is useful for multi-channel imaging.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    CyOFP1-TEV
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105782-plasmid-map-sequence-id-203371

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  34. pKK-mClover3-TEV

    Plasmid
    #105778
    Purpose
    Expression of your protein of interest in fusion with green fluorescent protein at the N-terminus (cleavable by TEV). mClover3 is a brighter derivative of mEGFP (PMID: 26879144).
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    mClover3-TEV
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105778-plasmid-map-sequence-id-203367

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  35. pKK-TEV-mVenus

    Plasmid
    #105791
    Purpose
    Expression of your protein of interest in fusion with yellow fluorescent protein at the C-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 11753368, 17040988, 22264545).
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-mVenus
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105791-plasmid-map-sequence-id-203382

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  42. pET24a-VHH-2xTS

    Plasmid
    #109419
    Purpose
    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to two tyrosine sulfation (TS) motifs. VHH-2xTS also contains a T7, HA, BAP and His6 epitope
    Depositor
    Martin Spiess
    Article
    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115
    Insert
    anti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
    Use
    Tags
    BAP, HA, His6, T7, and Tyrosine sulfation (TS) se…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    109419-plasmid-map-sequence-id-211590

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  46. pMB953:PB-CAG-PuroR-nGFP-FLEx-mCherry-KASH-WPRE

    Plasmid
    #168106
    Purpose
    PiggyBac transposon vector constitutively driving PuroR-P2A-eGFP and Cre conditional expression of KASH nuclear membrane-bound mCherry. Conditional insert flanked by MCS for cloning
    Depositor
    Erich Jarvis
    Article
    Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10
    Insert
    mCherry-KASH
    Use
    CRISPR and Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    CAG
    Available since
    May 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    168106-plasmid-map-sequence-id-330309

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  47. pMH-SFB-USP7

    Plasmid
    #99393
    Purpose
    Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged USP7
    Depositor
    Michael Huen
    Article
    An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114
    Insert
    USP7 (USP7 Human)
    Use
    Tags
    SFB (S protein-FLAG-Streptavidin binding peptide)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    99393-plasmid-map-sequence-id-190612

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  48. pSYC-437

    Plasmid
    #163829
    Purpose
    Foxj1a:GCaMP6s. Fluorescent reporter for calcium signaling (GCaMP6s) driven by zebrafish foxj1a promoter.
    Depositor
    Seok-Yong Choi
    Article
    Zhang et al Nat Commun. 2020 Apr 20;11(1):1860. doi:
    Inserts
    Foxj1a Promoter
    GCaMP6s
    Use
    Multisite gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    163829-plasmid-map-sequence-id-323196

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Showing: 3351 - 3400 of 4284 results