We narrowed to 1,410 results for: 4-Oct

1. RcSxph D794A

   Plasmid

   #194040

   Purpose

   Protein expression and characterization

   Depositor

   Dan Minor

   Article

   Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

   Insert

   Rana catesbeiana Saxiphilin
   

   Use

   Tags

   His10 and mEGFP,

   Expression

   Insect

   Mutation

   D794A

   Promoter

   Polyhedrin

   Available since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. RcSxph D794E

   Plasmid

   #194041

   Purpose

   Protein expression and characterization

   Depositor

   Dan Minor

   Article

   Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

   Insert

   Rana catesbeiana Saxiphilin
   

   Use

   Tags

   His10 and mEGFP,

   Expression

   Insect

   Mutation

   D794E

   Promoter

   Polyhedrin

   Available since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. RcSxph D794N

   Plasmid

   #194042

   Purpose

   Protein expression and characterization

   Depositor

   Dan Minor

   Article

   Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

   Insert

   Rana catesbeiana Saxiphilin
   

   Use

   Tags

   His10 and mEGFP,

   Expression

   Insect

   Mutation

   D794N

   Promoter

   Polyhedrin

   Available since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. RcSxph Y795A

   Plasmid

   #194043

   Purpose

   Protein expression and characterization

   Depositor

   Dan Minor

   Article

   Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

   Insert

   Rana catesbeiana Saxiphilin
   

   Use

   Tags

   His10 and mEGFP,

   Expression

   Insect

   Mutation

   Y795A

   Promoter

   Polyhedrin

   Available since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. RcSxph Y558A/I782A

   Plasmid

   #194044

   Purpose

   Protein expression and characterization

   Depositor

   Dan Minor

   Article

   Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

   Insert

   Rana catesbeiana Saxiphilin
   

   Use

   Tags

   His10 and mEGFP,

   Expression

   Insect

   Mutation

   Y558A/I782A

   Promoter

   Polyhedrin

   Available since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. KBB430

   Plasmid

   #185091

   Purpose

   NMA111-GFP with first nuclear localization signal mutated (amino acids 28 - 30, KRK -> AAA)

   Depositor

   Ken Belanger

   Article

   Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

   Insert

   NMA111
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   First nuclear localization signal mutated (amino …

   Promoter

   Available since

   July 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. KBB428

   Plasmid

   #185090

   Purpose

   NMA111-GFP with first nuclear localization signal mutated (amino acids 9 - 11, KKR -> AAA)

   Depositor

   Ken Belanger

   Article

   Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

   Insert

   NMA111
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   First nuclear localization signal mutated (amino …

   Promoter

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMKO.1-shSpc25

   Plasmid

   #160960

   Purpose

   Spc25 shRNA in pMKO.1 retroviral vector

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Spc25 shRNA (Spc25 Mouse)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMKO.1-shNuf2

   Plasmid

   #160961

   Purpose

   Nuf2 shRNA in pMKO.1 retroviral vector

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Nuf2 shRNA (Nuf2 Mouse)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLQ10873

    Plasmid

    #183961

    Purpose

    U6-SKSKSO(crRNA array)-CAG-hyperdCas12a-HA-miniVPR

    Depositor

    Stanley Qi

    Article

    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

    Inserts

    HyperdCas12a
    poly-crRNA array targeting Sox2-Klf4-Oct4
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D832A, D156R, E292R, D235R, D350R

    Promoter

    CAG and U6

    Available since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. t1-405(151-154AAAA)

    Plasmid

    #166131

    Purpose

    Expression of human talin-1 head (aa1-405) in E. coli. Four substitutions in the F1-loop (aa151-154 all mutated to Al). N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site.

    Depositor

    Vesa Hytönen

    Article

    Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13

    Insert

    T1head1-405(151-154AAAA) (TLN1 Human)
    

    Use

    Tags

    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…

    Expression

    Bacterial

    Mutation

    aa1-405 only. Contains 4 amino acid substitutions…

    Promoter

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCCL-dNTP63Promoter-FLuc-CMV-RLuc-dsRed2

    Plasmid

    #215326

    Purpose

    Lentiviral mammalian vector with firefly luciferase reporter for dNTP63 expression; renilla luciferase and dsRed2 reporter under CMV promoter.

    Depositor

    Rob Hynds

    Article

    Orr et al Am J Physiol Lung Cell Mol Physiol. 2024

    Insert

    dNTP63 Promoter
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dNTP63

    Available since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSEMS-CV SUy-Halo7Tag

    Plasmid

    #108933

    Purpose

    Selflabeling tag at ATP Synthase

    Depositor

    Karin Busch

    Article

    Wilkens et al J Cell Sci. 2013 Jan 1;126(Pt 1):103-16.

    Insert

    ATP synthase F1 subunit gamma (ATP5F1C Human)
    

    Use

    Tags

    Halo7 tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFRT/TO/FLAG/HA-DEST PABPC4

    Plasmid

    #19882

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

    Insert

    Poly(A) binding protein, cytoplasmic 4 (PABPC4 Human)
    

    Use

    Tags

    FLAG/HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRRL-pEF-H2B-mCherry-T2A-rTetR-Dest-SV40

    Plasmid

    #186968

    Purpose

    Lentiviral destination vector for cloning desired protein as rTetR fusion for chromatin regulation reporter (pEF-H2B-mCherry-T2A-rTetR, with SV40) for expression in mammalian cells.

    Depositor

    Brian Liau

    Article

    Lue et al Nat Chem Biol. 2023 Feb;19(2):176-186. d

    Insert

    pEF-H2B-mCherry-T2A-rTetR-Dest-SV40
    

    Use

    Lentiviral

    Tags

    rTetR fusion

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRRL-pEF-H2B-mCherry-T2A-rTetR-DNMT3L-SV40

    Plasmid

    #186967

    Purpose

    Lentiviral vector encoding DNMT3A recruiter for methylation reporter (pEF-H2B-mCherry-T2A-rTetR-DNMT3L-SV40) for expression in mammalian cells.

    Depositor

    Brian Liau

    Article

    Lue et al Nat Chem Biol. 2023 Feb;19(2):176-186. d

    Insert

    DNMT3L (DNMT3L Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    rTetR fusion

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMasterK

    Plasmid

    #165081

    Purpose

    Episomal expression of reprogramming factors.

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Stem Cell Res. 2021 May;53:102380. doi:

    Inserts

    OCT4 (POU5F1 Human)
    SOX2 (SOX2 Human)
    KLF4 (KLF4 Human)
    MYC (MYC Human)
    LRH1 (NR5A2 Human)
    NANOG (NANOG Human)
    lin28 (LIN28A Human)
    EGFP
    HSV TK
    KDM4D (KDM4D Callithrix jacchus)
    GLIS1 (GLIS1 Human)
    

    Use

    Episomal

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CD-MPR-GFP-RUSH

    Plasmid

    #202797

    Purpose

    RUSH cargo: Str-KDEL_IRES_SBP-EGFP-CD-MPR

    Depositor

    Juan Bonifacino

    Article

    Chen et al J Cell Biol. 2017 Dec 4;216(12):4141-415

    Insert

    M6PR (M6PR Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Changed 40 K (AAA) with R (AGA)

    Promoter

    CMV

    Available since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CD-MPR-mCherry-RUSH

    Plasmid

    #202798

    Purpose

    RUSH cargo: Str-KDEL_IRES_SBP-mCherry-CD-MPR

    Depositor

    Juan Bonifacino

    Article

    Chen et al J Cell Biol. 2017 Dec 4;216(12):4141-415

    Insert

    M6PR (M6PR Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Changed 40 K (AAA) with R (AGA)

    Promoter

    CMV

    Available since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pINTO-NFH::hSNRNP70

    Plasmid

    #65926

    Purpose

    Expression of human SNRNP70 fused to Flag-HA tag (N-terminus)

    Depositor

    Roberto Bonasio

    Article

    Beck et al J Proteome Res. 2014 Dec 5;13(12):6135-4

    Insert

    small nuclear ribonucleoprotein 70kDa (U1) (SNRNP70 Human)
    

    Use

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    Two silent mutations

    Promoter

    CMV/TO and CMV/TetO2 (T-REx)

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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