Addgene

Showing: 1341 - 1360 of 18404 results

Results per page:

Sort results by:

pAAV-hSyn-Chronos-mT-Sapphire-NRN

Plasmid

#149692

Purpose

For packaging and expression of Chronos-mT-Sapphire featuring a neuritin (NRN) 3' UTR in neurons via AAV

Depositor

Robert Malenka

Article

Christoffel et al Proc Natl Acad Sci U S A. 2021 Jun 15;11

Insert

Chronos

Use

AAV

Tags

mT-Sapphire

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDestTol2-actinb-kcnk9-IRES-EGFP

Plasmid

#164949

Purpose

Tol2 construct: actinb (actb2) promoter driven zebrafish kcnk9 and EGFP

Depositor

GuangJun Zhang

Article

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

kcnk9 (kcnk9 Zebrafish)

Use

Tol2 transposon destination vector

Tags

IRES-EGFP

Expression

Mutation

Promoter

actinb (actb2) promoter

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-mTurquoise-MLC-IRES-neo

Plasmid

#85145

Purpose

lentiviral expression of myosin light chain (MLC, MYL9), N-terminally tagged with mTurquoise

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

mTurquoise-MLC (myosin regulatory light chain, N-terminally tagged with mTurquoise (MYL9 Human)

Use

Lentiviral

Tags

mTurquoise

Expression

Mutation

Promoter

EF1a

Available since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB CAG SMARCA4-IRES-EGFP

Plasmid

#153948

Purpose

piggyBac transposon vector with CAG promoter expressing wild-type SMARCA4 (FLAG-tagged) and EGFP

Depositor

Luca Tiberi

Article

Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

Insert

SMARCA4 (SMARCA4 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRetroX-mCRT-GPI-IRES-DsRed

Plasmid

#223711

Purpose

Mammalian expression of mouse CRT-GPI

Depositor

Judy Lieberman

Article

Sen Santara et al Nature. 2023 Apr;616(7956):348-356. doi:

Insert

Calreticulin-GPI (Calr Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-TRE-DsRed-IRES-d24EGFP

Plasmid

#41945

Purpose

Retroviral expression of DsRed in mammalian cells

Depositor

Stephen Elledge

Article

Yen et al Science. 2008 Nov 7;322(5903):918-23. do

Insert

DsRed

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

May 2, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV eIF4E S209D IRES GFP

Plasmid

#18765

Depositor

Scott Lowe

Article

Wendel et al Genes Dev. 2007 Dec 15. 21(24):3232-7.

Insert

eIF4E S209D (Eif4e Mouse)

Use

Retroviral

Tags

IRES GFP

Expression

Mammalian

Mutation

S209D

Promoter

Available since

July 27, 2008

Availability

Academic Institutions and Nonprofits only
pDisplay-UDP1.0-IRES-mCherry-CAAX

Plasmid

#220850

Purpose

CMV promoter drives the expression of an extracellular UDP sensor (UDP1.0) and a membrane localized mCherry

Depositor

Yulong Li

Article

Umpierre et al Neuron. 2024 Apr 10:S0896-6273(24)00195-

Insert

UDP1.0

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-FLAG-mMdm2-IRES-GFP

Plasmid

#66955

Purpose

Retroviral vector to express a FLAG-tagged mouse Mdm2

Depositor

Martine Roussel

Insert

Mdm2 (Mdm2 Mouse)

Use

Retroviral

Tags

FLAG and IRES-GFP

Expression

Mammalian

Mutation

Promoter

MSCV

Available since

July 23, 2015

Availability

Academic Institutions and Nonprofits only
pLenti-EF1α-IRES-EGFP-hMCIDAS

Plasmid

#149696

Purpose

Expresses untagged human MCIDAS in mammalian cells

Depositor

Ken-Ichi Takemaru

Insert

MCIDAS (MCIDAS Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1 alpha

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
RRL.sin.cPPT.SFFV/Flag-E2-crimson.IRES-puro.WPRE (CG568)

Plasmid

#139445

Purpose

Lentiviral vector to ectopically express Flag-tagged E2-crimson from SFFV promoter

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

FLAG-tag E2-crimson

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLEX-FLAG-NRASQ61K-IRES-Puro

Plasmid

#120575

Purpose

Expresses Flag-N-Ras Q61K fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Q61K

Promoter

CMV

Available since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF1a-Ronin-FLAG-IRES-Neo

Plasmid

#28020

Depositor

Thomas Zwaka

Article

Dejosez et al Genes Dev. 2010 Jul 15. 24(14):1479-84.

Insert

Ronin (THAP11 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CamKIIa-moxBFP-IRES-Flpo

Plasmid

#194977

Purpose

AAV vector to drive expression of moxBFP and Flpo under the control of CamKIIalpha promoter

Depositor

Günter Schwarz

Article

Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

Insert

moxBFP, Flpo

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-EF1a-MinE-IRES-Blast

Plasmid

#213115

Purpose

Expression of untagged MinE in mammalian cells

Depositor

Scott Coyle

Article

Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

Insert

MinE (minE )

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIG-V5-Snail2-IRES-nls-GFP

Plasmid

#44282

Depositor

Martin Cheung

Article

Liu et al Proc Natl Acad Sci U S A. 2013 Feb 19;11

Insert

V5-Snail2 (SNAI2 Chicken)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV-IE-enhancer and chicken beta-actin promoter

Available since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-IRES-GFP-3xFLAG-MyD88-V5

Plasmid

#131351

Purpose

Expresses MyD88 tagged with a 3xFLAG tag at its N-terminus and a V5 tag at its C-terminus

Depositor

Jonathan Kagan

Article

Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

Insert

MyD88 (Myd88 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSH-EFIRES-P-Seipin-miniIAA7-mCherry

Plasmid

#129720

Purpose

AAVS1 safe harbor targeting vector expressing miniIAA7-mCherry tagged Seipin

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

Seipin (BSCL2 Human)

Use

CRISPR and TALEN; Human safe harbor locus (a avs1…

Tags

miniIAA7-mCherry

Expression

Mammalian

Mutation

silent mutations

Promoter

EF1a

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-HA-UBE3B(C1036A)-IRES-Puro

Plasmid

#107348

Purpose

Expresses HA tagged UBE3B mutant C1036A in mammalian cells.

Depositor

Robert Sobol

Article

Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

Insert

UBE3B(C1036A) (UBE3B Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

changed Cysteine 1036 to Alanine

Promoter

CMV

Available since

April 19, 2018

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLOC CMV-IRES-Dsred-blasticidin

Plasmid

#129428

Purpose

expression of Dsred-blasticidin under CMVpromoter -IRES

Depositor

Oskar Laur

Insert

Dsred-blasticidin

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge