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pAc5.1B-EGFP-DmNot1_1710-2505_J

Plasmid

#146701

Purpose

Insect Expression of DmNot1_1710-2505

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1_1710-2505 (Not1 Fly)

Use

Tags

Expression

Insect

Mutation

one non silent mutation (A1747T) and one silent m…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot1iso1_840-1605-IKGRQtoDSYAA_X

Plasmid

#148001

Purpose

Mammalian Expression of HsNot1iso1_840-1605-IKGRQtoDSYAA

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1_840-1605-IKGRQtoDSYAA (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

1 silent mutation compared to the sequence of iso…

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1_1318-1605-IKGRQtoDSYAA_W

Plasmid

#147895

Purpose

Mammalian Expression of HsNot1iso1_1318-1605-IKGRQtoDSYAA

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1_1318-1605-IKGRQtoDSYAA (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

1 silent mutation compared to the sequence of iso…

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot9-shRNAres2-H58AF60AA64YV71Y_W

Plasmid

#147906

Purpose

Mammalian Expression of HsNot9-shRNAres2-H58AF60AA64YV71Y

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot9-shRNAres2-H58AF60AA64YV71Y (CNOT9 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1-del1097-1110_W

Plasmid

#147836

Purpose

Mammalian Expression of HsNot1iso1-del1097-1110

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1-del1097-1110 (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

1 silent mutation compared to the sequence of iso…

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-EGFP-C1-HsNot1iso1-IKGIRQtoDSYDAA-shRNAres_W

Plasmid

#147837

Purpose

Mammalian Expression of HsNot1iso1-IKGIRQtoDSYDAA-shRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1-IKGIRQtoDSYDAA-shRNAres (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

1 silent mutation compared to the sequence of iso…

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-EGFP-C1-HsNot1iso1_1318-1605-IKGIRQtoDSYDAA_W

Plasmid

#147894

Purpose

Mammalian Expression of HsNot1iso1_1318-1605-IKGIRQtoDSYDAA

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1_1318-1605-IKGIRQtoDSYDAA (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

1 silent mutation compared to the sequence of iso…

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmNot1_1710-2505_J

Plasmid

#146702

Purpose

Insect Expression of DmNot1_1710-2505

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1_1710-2505 (Not1 Fly)

Use

Tags

Expression

Insect

Mutation

two non silent mutations (A1747T, G1999S) and one…

Promoter

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1-K1537SG1562EQ1658A-dsRNAres_W

Plasmid

#147860

Purpose

Insect Expression of DmNot1-K1537SG1562EQ1658A-dsRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1-K1537SG1562EQ1658A-dsRNAres (Not1 Fly)

Use

Tags

Expression

Insect

Mutation

10 silent mutations and two non silent N605S, K75…

Promoter

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_812-1945-dsRNAres_W

Plasmid

#147845

Purpose

Insect Expression of DmNot1_812-1945-dsRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1_812-1945-dsRNAres (Not1 Fly)

Use

Tags

Expression

Insect

Mutation

3 silent mutations compared to the sequence given…

Promoter

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1-KGRQtoSYAA-shRNAres_W

Plasmid

#147901

Purpose

Mammalian Expression of HsNot1iso1-KGRQtoSYAA-shRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1-KGRQtoSYAA-shRNAres (CNOT1 Human)

Use

Tags

GFP (G4C mutation)

Expression

Mammalian

Mutation

NM_016284.4, K1426S, G1451Y, R1458A, Q1549A

Promoter

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAW-BiP-Nb127D01-mCherry-KDEL

Plasmid

#171575

Purpose

Expresses mCherry-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-mCherry) in fly cell ER membrane

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

BiP-Nb127D01-mCherry-KDEL (CXCR2 Human)

Use

Tags

BiP signal peptide and mCherry-KDEL

Expression

Insect

Mutation

Promoter

fly actin5C promoter

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pSUMO GLTP

Plasmid

#170732

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Ochoa-Lizarralde et al J Biol Chem. 2018 Oct 26;293(43):16709-1

Insert

GLTP (GLTP Human)

Use

Tags

SUMO

Expression

Bacterial

Mutation

Promoter

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pAW-BiP-NbVHH05-mCherry-KDEL

Plasmid

#171574

Purpose

Expresses mCherry-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-mCherry) in fly cell ER membrane

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

BiP-NbVHH05-mCherry-KDEL (UBE2J1 Human)

Use

Tags

BiP signal peptide and mCherry-KDEL

Expression

Insect

Mutation

Promoter

fly actin5C promoter

Available since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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mTet3 flox donor

Plasmid

#117146

Purpose

Making Tet3 conditional knock out

Depositor

Jacob Hanna

Article

Zviran et al ssrn.3155731

Insert

Tet3 (Tet3 Mouse)

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Plxnb2(S)-AP-His

Plasmid

#72003

Purpose

Expresses the N-terminal extracellular region of the PlexinB2 protein following proteolytic cleavage (ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxnb2 (Plxnb2 Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

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Jam4.3-Fc-His

Plasmid

#72082

Purpose

Expresses the extracellular region of the JAM-4, isoform 3 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Jam4.3 (Igsf5 Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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Jam3-Fc-His

Plasmid

#72080

Purpose

Expresses the extracellular region of the JAM-3 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Jam3 (Jam3 Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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Lrrc4-AP-His

Plasmid

#71958

Purpose

Expresses the extracellular region of the LRRC4 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Lrrc4 (Lrrc4 Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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Jam3-AP-His

Plasmid

#71954

Purpose

Expresses the extracellular region of the JAM-3 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Jam3 (Jam3 Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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