We narrowed to 2,616 results for: pCas
-
Plasmid#223022PurposeTemplate for in vitro transcription of PE7. For HSPC-related experiments.DepositorInsertPE7
UseTemplate for in vitro transcriptionTagsSV40 bpNLS and c-Myc NLSPromoterT7Available sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpyCas9 PE2*
Plasmid#169850PurposeMammalian Expression, SpyCas9 prime editor, optimized NLSDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertspCas9 PE*
TagsbpSV40 NLS and SV40 NLS and cmyc-NLS and bpSV40 N…ExpressionMammalianPromotercmvAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p458 VQR
Plasmid#101727PurposeExpresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSpCas9 VQR
ExpressionMammalianMutationVQRAvailable sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pNDamMyc
Plasmid#59217PurposeDrosophila DamID vector used to fuse gene of interest to Myc-tagged E.coli Dam.DepositorTypeEmpty backboneUseDamidTagsDam and MycExpressionInsectPromoterDrosophila heat-shock promoterAvailable sinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBECKP-spe
Plasmid#117236Purposencas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickaseDepositorInsertncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRDA_426
Plasmid#179097PurposeBE Cas expressionDepositorInsertABE8e
UseCRISPR and LentiviralAvailable sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CANE-LV envelope
Plasmid#86666PurposepCAGGS/ES-M21EnvA-VSVg-WPRE, used to package pseudotyped lentivirusDepositorInsertCANE-LV envelope
UseLentiviral, Mouse Targeting, and Synthetic Biolog…ExpressionMammalianPromoterCAGAvailable sinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCutamp
Plasmid#140632PurposePlasmid-curing in Escherichia coli by targeting the AmpR promoterDepositorInsertSpCas9_lambda-RED system, SacB, Rha induction system, sgRNA targeting AmpR promoter
UseCRISPRExpressionBacterialAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-BE4max-NRCH
Plasmid#136920PurposeExpresses BE4max-NRCH in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBE4max-NRCH
UseCRISPRExpressionMammalianMutationsee manuscriptPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
p2T-CMV-eA3A-BE5-NG-BlastR
Plasmid#152998PurposeHigh purity C-to-T base editorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserteA3A-BE4 T44D+S45A-nSpCas9-UGI-UGI
UseCRISPRExpressionMammalianPromoterCMVAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-1BPNLS-Cas9-1BPNLS-2AGFP
Plasmid#87109PurposeBPNLS-Cas9-BPNLS-2AGFP expression plasmidDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert1BPNLS-Cas9-1BPNLS-2A-EGFP
UseCRISPRTags3xFLAG (on Cas9)ExpressionMammalianPromoterCAGAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRDA_336
Plasmid#179095PurposeBE Cas expressionDepositorInsertBE3.9 (Cas9-NG)
UseCRISPR and LentiviralAvailable sinceJan. 19, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX458-Ef1a-Cas9-H2B-GFP
Plasmid#159654PurposeEmpty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B GFP nuclear reporter.DepositorTypeEmpty backboneUseCRISPRAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEM-Cas9HF1
Plasmid#89961PurposeModified from pCas9-CR4 (Addgene: 62655) to use the high-fidelity version of Cas9, SpCas9-HF1 (N497A/R661A/Q695A/Q926A) from Kleinstiver et al 2016.DepositorInsertCas9HF1
ExpressionBacterialMutationN497A/R661A/Q695A/Q926APromoterpTetAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
hAAVS1-gs4_EF1a-Cas9-2A-GFP
Plasmid#118414PurposeCRISPR knockout, expresses Cas9, and sgRNA targeting AAVS1 locusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA AAVS1 (AAVS1 Human)
UseCRISPRExpressionMammalianAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pC0051-W85X REPAIR targeting guide cloned into pC0043
Plasmid#103867PurposeGuide RNA expression plasmid for dPspCas13b that can be used to direct ADAR deaminase domain activity to the W85X mutation in the luciferase reporter plasmid pC0038DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertW85X targeting guide RNA
UseCRISPRExpressionMammalianAvailable sinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330-sgR26
Plasmid#127376PurposeUsed for contransfection with pR26- and pR26-CMVconst-derived constructs to mediate CRISPR/Cas9 genomic insertion into the murine ROSA26 safe harbor locus.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertROSA26 (Gt(ROSA)26Sor Mouse)
UseCRISPR and Mouse TargetingAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pL-CRISPR.SFFV.PAC
Plasmid#57829PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, SFFV Promoter drivenDepositorInsertsSpCas9
Sp sgRNA scaffold
SFFV
P2A-PAC
UseCRISPR and LentiviralTagsFLAGAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pC-sleeping beauty
Plasmid#65487PurposeHSP-driven SB100X Sleeping Beauty transposase in pCasper backbone.DepositorInsertsleeping beauty transposase
ExpressionInsectPromoterHSPAvailable sinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMonAID_nCas9-PmCDA_Hyg_ALS
Plasmid#91693PurposeMonocot Target-AID vector expressing rice-optimized nCas9-PmCDA1 with sgRNA targeting OsAlsDepositorInsertSpCas9
TagsPmCDA1ExpressionPlantMutationD10A for nickase Cas9Promoter2x35SAvailable sinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only