We narrowed to 2,598 results for: pCas

Showing: 1341 - 1360 of 2598 results

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pCAG-1BPNLS-Cas9-1BPNLS-2AGFP

Plasmid

#87109

Purpose

BPNLS-Cas9-BPNLS-2AGFP expression plasmid

Depositor

Juan Belmonte

Publication

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

1BPNLS-Cas9-1BPNLS-2A-EGFP

Use

CRISPR

Tags

3xFLAG (on Cas9)

Expression

Mammalian

Promoter

CAG

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pBECKP-spe

Plasmid

#117236

Purpose

ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase

Depositor

Quanjiang Ji

Publication

Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

Insert

ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV-BE4max-NRCH

Plasmid

#136920

Purpose

Expresses BE4max-NRCH in mammalian cells

Depositor

David Liu

Publication

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

BE4max-NRCH

Use

CRISPR

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pRDA_336

Plasmid

#179095

Purpose

BE Cas expression

Depositor

John Doench, David Root

Publication

Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

Insert

BE3.9 (Cas9-NG)

Use

CRISPR and Lentiviral

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pX458-Ef1a-Cas9-H2B-GFP

Plasmid

#159654

Purpose

Empty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B GFP nuclear reporter.

Depositor

Joseph Brzezinski

Publication

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Type

Empty backbone

Use

CRISPR

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pEM-Cas9HF1

Plasmid

#89961

Purpose

Modified from pCas9-CR4 (Addgene: 62655) to use the high-fidelity version of Cas9, SpCas9-HF1 (N497A/R661A/Q695A/Q926A) from Kleinstiver et al 2016.

Depositor

Michael Lynch

Publication

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

Cas9HF1

Expression

Bacterial

Mutation

N497A/R661A/Q695A/Q926A

Promoter

pTet

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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hAAVS1-gs4_EF1a-Cas9-2A-GFP

Plasmid

#118414

Purpose

CRISPR knockout, expresses Cas9, and sgRNA targeting AAVS1 locus

Depositor

Mark Denham

Publication

Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

Insert

gRNA AAVS1 (AAVS1 Human)

Use

CRISPR

Expression

Mammalian

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pC0051-W85X REPAIR targeting guide cloned into pC0043

Plasmid

#103867

Purpose

Guide RNA expression plasmid for dPspCas13b that can be used to direct ADAR deaminase domain activity to the W85X mutation in the luciferase reporter plasmid pC0038

Depositor

Feng Zhang

Publication

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Insert

W85X targeting guide RNA

Use

CRISPR

Expression

Mammalian

Available since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCutamp

Plasmid

#140632

Purpose

Plasmid-curing in Escherichia coli by targeting the AmpR promoter

Depositor

Sheng Yang

Publication

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

SpCas9_lambda-RED system, SacB, Rha induction system, sgRNA targeting AmpR promoter

Use

CRISPR

Expression

Bacterial

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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px330_Rosa_sgRNA

Plasmid

#97007

Purpose

Expresses Cas9 and Rosa26 locus specific sgRNA

Depositor

Russell Ray

Publication

Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

Insert

Rosa26 sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pL-CRISPR.SFFV.PAC

Plasmid

#57829

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, SFFV Promoter driven

Depositor

Benjamin Ebert

Publication

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
SFFV
P2A-PAC

Use

CRISPR and Lentiviral

Tags

FLAG

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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CP1041-ABE8e

Plasmid

#138493

Purpose

A-to-G base editor

Depositor

David Liu

Publication

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(8e)-nSpCas9 (CP1041)

Expression

Mammalian

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pC-sleeping beauty

Plasmid

#65487

Purpose

HSP-driven SB100X Sleeping Beauty transposase in pCasper backbone.

Depositor

Guillaume Filion

Insert

sleeping beauty transposase

Expression

Insect

Promoter

HSP

Available since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMonAID_nCas9-PmCDA_Hyg_ALS

Plasmid

#91693

Purpose

Monocot Target-AID vector expressing rice-optimized nCas9-PmCDA1 with sgRNA targeting OsAls

Depositor

Akihiko Kondo

Publication

Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

SpCas9

Tags

PmCDA1

Expression

Plant

Mutation

D10A for nickase Cas9

Promoter

2x35S

Available since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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pICSL11056

Plasmid

#68258

Purpose

Level 1 Golden Gate Cassette: Cas9 expression cassette for monocotyledonous plants

Depositor

Nicola Patron

Publication

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter/5UTR: ZmUbi + CDS:SpCas9 + 3UTR/terminator:35s

Use

Synthetic Biology

Expression

Plant

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pRDA_429

Plasmid

#179098

Purpose

BE Cas expression

Depositor

John Doench, David Root

Publication

Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

Insert

ABE8e (Cas9-NG)

Use

CRISPR and Lentiviral

Available since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pX330-sgR26

Plasmid

#127376

Purpose

Used for contransfection with pR26- and pR26-CMVconst-derived constructs to mediate CRISPR/Cas9 genomic insertion into the murine ROSA26 safe harbor locus.

Depositor

Lance Miller

Publication

Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1

Insert

ROSA26 (Gt(ROSA)26Sor Mouse)

Use

CRISPR and Mouse Targeting

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p101_CRISPRai_PiggyBac

Plasmid

#213777

Purpose

Expresses Tet-On inducible CRISPRai orthogonal system with VPR-dSaCas9, dSpCas9-KRAB-BFP, zeocin resistance gene, and Tet-On transactivator. PiggyBac vector.

Depositor

Howard Chang

Publication

Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

Inserts

VPR-dSaCas9
dSpCas9-KRAB-BFP
zeocin
Tet-On transactivator

Use

CRISPR

Promoter

EF1a (EF-1-alpha intron a) and Tet_On_TRE

Available since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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AAEL010097-Cas9

Plasmid

#100707

Purpose

The promoter of AAEL010097 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

Depositor

Omar Akbari

Publication

Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

Inserts

AAEL010097-hspcas9-GFP
Opie2-dsRed

Use

CRISPR

Tags

GFP and dsRED

Expression

Insect

Promoter

AAEL010097 and Opie2

Available since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMH5

Plasmid

#52531

Purpose

expresses FLP recombinase in Drosophila

Depositor

Klaus Foerstemann

Publication

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Insert

FLP recombinase (flp Budding Yeast)

Expression

Insect

Promoter

ubiquitin

Available since

April 23, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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