We narrowed to 5,817 results for: pCas

1. pX330_rSyn1 sgRNA / hSpCas9

   Plasmid

   #172840

   Purpose

   Mammalian expression of a sgRNA targeting the intron 21 (last intron) of rSyn1 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting intron 21 of rSyn1 under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG/hArf4(WT)-EGFP

   Plasmid

   #79407

   Purpose

   Expresses C-terminally EGFP-tagged Arf4(WT) in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Nakai et al Mol Biol Cell. 2013 Aug;24(16):2570-81.

   Insert

   Arf4 (ARF4 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

3. pGL3-B-838;+80pCalb2

   Plasmid

   #66750

   Purpose

   luciferase reporter for Calb2 promoter (-838 to +80, WT)

   Depositor

   Rolf Stahel, Emanuela Felley-Bosco

   Article

   Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

   Insert

   Calb2 promoter (-838bp +80) (CALB2 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Promoter

   Calb2

   Available Since

   Jan. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGGS-S-501V2-FLAG

   Plasmid

   #177096

   Purpose

   501V2 variant S-protein with c-terminal FLAG tag

   Depositor

   Daniel Conway

   Insert

   S-protein 501V2 (S SARS-CoV-2)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Nov. 2, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pSpCas9_BB_2A-GFP_MAPRE3-gRNA#3

   Plasmid

   #107731

   Purpose

   Knock out of EB3 in human cells by CRISPR/Cas9

   Depositor

   Torsten Wittmann

   Article

   van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

   Insert

   MAPRE3 gRNA #3 (targets Exon 1) (MAPRE3 Human)
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpCas9_BB_2A-GFP_MAPRE1-gRNA#3

   Plasmid

   #107728

   Purpose

   Knock out of EB1 in human cells by CRISPR/Cas9

   Depositor

   Torsten Wittmann

   Article

   van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

   Insert

   MAPRE1 gRNA #3 (targets Exon 1) (MAPRE1 Human)
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. BII-ChBtW-eSpCas9

   Plasmid

   #133370

   Purpose

   Piggybac vector for constitutive expression

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   eSpCas9
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   eSpCas9 mutations

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS-S-B.1.617.1-FLAG

   Plasmid

   #177098

   Purpose

   B.1.617.1 variant S-protein with c-terminal FLAG tag

   Depositor

   Daniel Conway

   Insert

   S-protein B.1.617.1 (S SARS-CoV-2)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Nov. 2, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pFREE2-eSpCas9-sgRNA

   Plasmid

   #179580

   Purpose

   Expresses eSpCas9 and gRNA that targets ColE1 plasmids for curing

   Depositor

   Kevin Solomon

   Article

   Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

   Insert

   eSpCas9
   

   Expression

   Bacterial

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-HDAC4 1-747

    Plasmid

    #71399

    Purpose

    CAG promoter, mammalian expression

    Depositor

    Bo Chen

    Article

    Guo et al Nat Commun. 2015 Aug 14;6:8005. doi: 10.

    Insert

    HDAC4 1-747 (HDAC4 Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pBT225_(pCA-tdT3Myc)

    Plasmid

    #36873

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Insert

    tdTomato-3Myc
    

    Tags

    3 Myc tags

    Expression

    Mammalian

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer)

    Available Since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pACYC-OspCas12c-noncoding

    Plasmid

    #120878

    Purpose

    Expresses concatenated noncoding sequences surrounding the CRISPR-OspCas12c system in the native genomic loci; expresses the tracrRNA required for activity.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

    Insert

    Noncoding sequences for CRISPR-OspCas12
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-SRGAP3FBAR-EGFP

    Plasmid

    #168506

    Purpose

    Expression of F-BAR domain (aa 1-492) of SRGAP3 with a C-terminal EGFP fusion

    Depositor

    Franck Polleux

    Article

    Coutinho-Budd et al J Cell Sci. 2012 Jul 15;125(Pt 14):3390-

    Insert

    SRGAP3-FBAR (SRGAP3 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa 1-492

    Promoter

    CAG

    Available Since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-AmCas12b-2AeGFP

    Plasmid

    #121947

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    AmCas12b
    

    Tags

    3xHA tag

    Expression

    Mammalian

    Available Since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSpCas9(BB)-2A-Intron4-PAFAH1B1

    Plasmid

    #92434

    Purpose

    expresses Cas9 and gRNA targeting PAFAH1B1

    Depositor

    Ralf Brandes, Matthias Leisegang

    Article

    Josipovic et al Acta Physiol (Oxf). 2016 Apr 28. doi: 10

    Insert

    PAFAH1B1 (PAFAH1B1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-TetR-RNF169UBD

    Plasmid

    #119011

    Purpose

    Mammalian expression of a fusion protein of single chain Tet repressor with the human RNF169 Ubiquitin binding domain and BFP expression

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    TetR
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCA24N-ligase-pprA

    Plasmid

    #87744

    Purpose

    IPTG-inducible expression of ligase-pprA fusion for protein purification

    Depositor

    Wayne Patrick

    Article

    Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

    Insert

    T4 DNA ligase (30 )
    

    Tags

    6xHis and codon optimized pprA (from Deinococcus …

    Expression

    Bacterial

    Promoter

    T5-lac

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCaSpeR-act5cB-LexAQFw

    Plasmid

    #61311

    Purpose

    Expresses chimeric transactivator LexA_DBD:QF2w_AD in Drosophila

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    LexA_DBD::QF2w_AD
    

    Expression

    Insect

    Mutation

    Changed EK on C-terminus of QF2 to KKKK

    Available Since

    Jan. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCALNL-BsaI-EGFP

    Plasmid

    #123136

    Purpose

    Golden Gate acceptor vector for the assembly of EGFP-based mammalian recombinase reporters.

    Depositor

    David Liu

    Article

    Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

    Insert

    EGFP, mRFP
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    cBA

    Available Since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG aconitase GFP

    Plasmid

    #66104

    Purpose

    expression of chicken aconitase GFP

    Depositor

    Martin Scaal, Felicitas Proels

    Article

    et al Development. 2015 Feb 15;142(4):665-71.

    Insert

    aconitase
    

    Use

    Chicken expression

    Tags

    EGFP

    Promoter

    CAG

    Available Since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only