We narrowed to 16,751 results for: EMB

1. pHA852 - mec-4p::FynY531F

   Plasmid

   #139209

   Purpose

   Expresses activated Fyn kinase in mec-4 neurons

   Depositor

   Anne Hart

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   Fyn (FYN Human)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Y531F

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. TOG_D1

   Plasmid

   #139112

   Purpose

   express first domain (1-250) of TOG

   Depositor

   Nicolas Fawzi

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   TOG/CKAP5 (CKAP5 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   1-250

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. hnRNPA2_LC_RtoK

   Plasmid

   #139120

   Purpose

   express hnRNPA2 LC where all R are K

   Depositor

   Nicolas Fawzi

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   hnRNPA2 (HNRNPA2B1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   all R to K

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. hnRNPA2_LC_NtoS

   Plasmid

   #139121

   Purpose

   express hnRNPA2 with all N changed to S

   Depositor

   Nicolas Fawzi

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   hnRNPA2 (HNRNPA2B1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   all N to S

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. hnRNPA2_LC_5E

   Plasmid

   #139122

   Purpose

   express hnRNPA2 with 5 serine changed to glutamate

   Depositor

   Nicolas Fawzi

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   hnRNPA2 (HNRNPA2B1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   5 serine to glutamate

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. hnRNPA2_LC_12E

   Plasmid

   #139123

   Purpose

   express hnRNPA2 with all serine change to glutamate

   Depositor

   Nicolas Fawzi

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   hnRNPA2 (HNRNPA2B1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   12 S to E

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. hnRNPF_PLD_YtoS

   Plasmid

   #139124

   Purpose

   express hnRNPF PLD with all Y changed to S

   Depositor

   Nicolas Fawzi

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   hnRNPA2 (HNRNPA2B1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   all Y to S

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. hnRNPF_PLD_StoA

   Plasmid

   #139125

   Purpose

   express hnRNPF PLD with all S changed to A

   Depositor

   Nicolas Fawzi

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   hnRNPA2 (HNRNPA2B1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   all S to A

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GFP-Miro1 (184-618)

   Plasmid

   #127626

   Purpose

   Human Miro1 lacking the first GTPase domain

   Depositor

   Josef Kittler

   Article

   Covill-Cooke et al EMBO Rep. 2020 Feb 5;21(2):e49865. doi:

   Insert

   Miro1 (RHOT1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   184-618 only

   Promoter

   Available since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FB010

    Plasmid

    #119708

    Purpose

    Transcriptional Unit (TU) for hygromicin resistance in fungi obtained by combining FB001+GB0211+FB002 into pDGB3alpha1. According to FungalBraid/GoldenBraid modular DNA assembly for ATMT

    Depositor

    Jose Marcos

    Article

    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

    Insert

    PtrpC:hph:Ttub
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. NET39 Short pmRFP-N2 (451)

    Plasmid

    #61981

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

    Insert

    NET39 (PPAPDC3 Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Short form of NET39

    Promoter

    CMV

    Available since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

12. CC1

    Plasmid

    #63713

    Purpose

    Wild-type voltage sensing domain from Ciona voltage sensing phosphatase fused to super ecliptic A227D with restriction site flanking each transmembrane domain

    Depositor

    Bradley Baker

    Article

    Piao et al J Neurosci. 2015 Jan 7;35(1):372-85. doi

    Insert

    CC1
    

    Use

    Tags

    Super ecliptic pHlorin A227D

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pMM2-hPMCA4bct120

    Plasmid

    #47760

    Purpose

    mammalian expression of human PMCA4 with C-terminal 120aa deletion

    Depositor

    Emanuel Strehler, John Penniston

    Article

    Enyedi et al J Biol Chem. 1993 May 15;268(14):10621-6

    Insert

    PMCA4bct120 (ATP2B4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deletion of C-terminal 120 aa (removes calmodulin…

    Promoter

    SV40

    Available since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pIRES:hTRAAK(W262S)

    Plasmid

    #133078

    Purpose

    Mammalian expression vector. It will generate the human TRAAK channel full lenght W262S mutant

    Depositor

    Dan Minor

    Article

    Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

    Insert

    Potassium channel subfamily K member 4 (KCNK4, TRAAK) W262S (KCNK4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pIRES:hTRAAK(G124I)

    Plasmid

    #133079

    Purpose

    Mammalian expression vector. It will generate the human TRAAK channel full lenght G124I mutant

    Depositor

    Dan Minor

    Article

    Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

    Insert

    Potassium channel subfamily K member 4 (KCNK4, TRAAK) G124I (KCNK4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G124I

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAV10.K3.loxP

    Plasmid

    #63202

    Purpose

    pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAV10.K6.loxP

    Plasmid

    #63203

    Purpose

    pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAV10.K5.loxP

    Plasmid

    #63204

    Purpose

    pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAV10.HO3.loxP

    Plasmid

    #63205

    Purpose

    pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAV10.HO6

    Plasmid

    #63211

    Purpose

    pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into HO locus (NotI or BciVI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pF(UG) hSyn NS34a-msGFP-A'Q-mito

    Plasmid

    #158766

    Purpose

    Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.

    Depositor

    Edwin Chapman

    Article

    Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

    Insert

    NS3/4a protease and msGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Flexible linkers between the protease and msGFP a…

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pF(UG) hSyn NS34a-msGFP-'Q-mito

    Plasmid

    #158767

    Purpose

    Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.

    Depositor

    Edwin Chapman

    Article

    Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

    Insert

    NS3/4a protease and msGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Flexible linkers between the protease and msGFP a…

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEGFP-2xFYVE

    Plasmid

    #140047

    Purpose

    Expresses GFP-tagged HRS 2xFYVE in mammalian cells

    Depositor

    Harald Stenmark

    Article

    Gillooly et al EMBO J. 2000 Sep 1;19(17):4577-88. doi:

    Insert

    tandem HRS FYVE domain (Hgs Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    two FYVE domains (amino acids 147-223), linked by…

    Promoter

    CMV

    Available since

    May 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMP71-tCD19-mGFP

    Plasmid

    #174609

    Purpose

    expresses membrane-associated (palmitoylated) EGFP and extracellular and transmembrane domains of murine CD19

    Depositor

    Stanley Riddell

    Article

    Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

    Insert

    CD19 extracellular and transmembrane domain (Cd19 Mouse)
    

    Use

    Tags

    palmitoylated EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDN34-BiNLS-hYAP1-1delta

    Plasmid

    #169471

    Purpose

    expresses optoYAP in mammalian cells

    Depositor

    Timothy Saunders

    Article

    Toh et al EMBO Rep. 2022 Sep 5;23(9):e54401. doi:

    Insert

    YAP1-1delta (YAP1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEGFP-hGal3

    Plasmid

    #73080

    Purpose

    Expression in mammalian cells

    Depositor

    Tamotsu Yoshimori

    Article

    Maejima et al EMBO J. 2013 Aug 28;32(17):2336-47. doi:

    Insert

    Galectin-3 (LGALS3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAG26

    Plasmid

    #226743

    Purpose

    Expresses a reporter for a mitochondrial intermembrane space protein

    Depositor

    Jonathan Weissman

    Article

    Guna et al Science. 2022 Oct 21;378(6617):317-322.

    Insert

    MICU1 (targeting sequence) (MICU1 Human)
    

    Use

    Lentiviral

    Tags

    GFP1-10

    Expression

    Mutation

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. GST PTBP1 isoform 4

    Plasmid

    #108594

    Purpose

    Recombinant GST tagged protein production

    Depositor

    Chris Smith

    Article

    Coelho et al EMBO J. 2015 Mar 4;34(5):653-68. doi: 10

    Insert

    PTBP1 isoform 4 (PTBP1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    isoform 4 (isoform a)

    Promoter

    tac

    Available since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. TPC1-mCherry

    Plasmid

    #135182

    Purpose

    Expresses TPC1 with C-terminus mCherry tag in mammalian cells

    Depositor

    Antony Galione

    Article

    Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

    Insert

    TPC1 (Tpcn1 Mouse)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1/CIP2A(1-905) WT V5 His

    Plasmid

    #119287

    Purpose

    CIP2A WT V5 overexpression

    Depositor

    Jukka Westermarck

    Article

    Wang et al EMBO Rep. 2017 Mar;18(3):437-450. doi: 1

    Insert

    KIAA1524 (CIP2A Human)
    

    Use

    Tags

    V5 His

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pSCIP-hCD69-TdTomato

    Plasmid

    #154095

    Purpose

    Self-Cutting and Integrating CRISPR backbone targeting human CD69 promoter with TdTomato reporter transgene

    Depositor

    Scott McComb

    Article

    Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

    Inserts

    [5HA]-P2A-tdTomato-[3HA]
    hCD69 targeting sgRNA - AGCTCTTTGCATCCGGAGAG
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3-Lyn-ICUE3

    Plasmid

    #61623

    Purpose

    Plasma membrane-targeted FRET biosensor for monitoring cAMP

    Depositor

    Jin Zhang

    Article

    DiPilato et al Mol Biosyst. 2009 Aug;5(8):832-7. doi: 1

    Insert

    Lyn-ICUE3 (RAPGEF3 Human)
    

    Use

    Tags

    ECFP, N-terminal targeting sequence from Lyn kina…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pQFlag-USP7 WT puroR

    Plasmid

    #46751

    Purpose

    Retroviral vector that expresses wild type Flag-tagged human USP7

    Depositor

    Goedele Maertens, Gordon Peters

    Article

    Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

    Insert

    USP7 (USP7 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3-HA-p53 2KR

    Plasmid

    #78799

    Purpose

    To overexpress p53 2KR in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K291R, K292R

    Promoter

    CMV

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

35. pcDNA5-FRT-TO-NOD1-EGFP

    Plasmid

    #131206

    Purpose

    mammalian expression, tet R

    Depositor

    Thomas Kufer

    Article

    Pei et al EMBO J. 2021 Jul 1;40(13):e106272. doi:

    Insert

    NOD1 (NOD1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEGFP VARP

    Plasmid

    #42312

    Depositor

    Thierry Galli

    Article

    Burgo et al EMBO Rep. 2009 Oct;10(10):1117-24. doi:

    Insert

    VARP (ANKRD27 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 14, 2013

    Availability

    Academic Institutions and Nonprofits only

37. TPC2-mCherry

    Plasmid

    #135183

    Purpose

    Expresses TPC2 with C-terminus mCherry tag in mammalian cells

    Depositor

    Antony Galione

    Article

    Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

    Insert

    TPC2 (Tpcn2 Mouse)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. OMM-long-CFAST10

    Plasmid

    #233598

    Purpose

    Expression of CFAST10 on the OMM membrane after a long linker

    Depositor

    Riccardo Filadi

    Article

    Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

    Insert

    CFAST10
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCMV-EGFP-eDHFR(69K6)-MCS

    Plasmid

    #172100

    Purpose

    Mammalian expression of a protein of interest fused to the C-terminus of EGFP-eDHFR(69K6)

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    EGFP-eDHFR(69K6)-20aa
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    eDHFR(69K6): Hexalysine (K6) sequence is inserted…

    Promoter

    CMV

    Available since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pQFlag-USP7 CS puroR

    Plasmid

    #46752

    Purpose

    Retroviral vector that expresses catalytically inactive form of Flag-tagged human USP7

    Depositor

    Goedele Maertens, Gordon Peters

    Article

    Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

    Insert

    USP7 (USP7 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    C223S--catalytically inactive

    Promoter

    CMV

    Available since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge