We narrowed to 2,098 results for: Pam

1. pAAV-Ef1a-DIO-Sun1GFP-WPRE-pA

   Plasmid

   #160141

   Purpose

   Cre- dependent expression of Sun1GFP for nuclei isolation

   Depositor

   Andreas Pfenning

   Article

   Lawler et al J Neurosci. 2020 Nov 10. pii: JNEUROSCI.

   Insert

   Sun1-GFP (Sun1 Mouse)
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   N-truncated: removed amino acids 1-207, retained …

   Promoter

   Ef1a

   Available since

   March 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. TRE3G-PE2-P2A-BFP

   Plasmid

   #231580

   Purpose

   Expresses the PE2 prime-editing machinery fused to BFP (PE2-P2A-BFP), under the control of a TRE3G promoter. This promoter is responsive to doxycycline bound to the rtTA protein

   Depositor

   Jesse Engreitz

   Article

   Martyn et al bioRxiv [Preprint]. 2023 Dec 21:2023.12.

   Insert

   PE2-P2A-BFP
   

   Use

   Lentiviral

   Tags

   P2A-BFP

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-T7-SpCas9-NG-P2A-EGFP (RTW3677)

   Plasmid

   #139994

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…

   Promoter

   CMV and T7

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-T7-ABEmax(7.10)-SpCas9-NG-P2A-EGFP (RTW4564)

   Plasmid

   #140005

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized ABEmax(7.10) SpCas9-NG with P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…

   Promoter

   CMV and T7

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA

   Plasmid

   #154867

   Purpose

   Flp-dependent hM4D(Gi) DREADD-mCherry

   Depositor

   Ulrik Gether

   Article

   Runegaard et al eNeuro. 2018 Jun 18;5(3). pii: eN-NWR-03

   Has Service

   AAV Retrograde and AAV8

   Insert

   hM4D(Gi)-mCherry
   

   Use

   AAV; Flp-frt

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Human Synapsin-1

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-T7-SpG-P2A-EGFP (RTW4177)

   Plasmid

   #139988

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9 variant named SpG with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

   Promoter

   CMV and T7

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-T7-xCas9(3.7)-P2A-EGFP (RTW3576)

   Plasmid

   #139993

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized xCas9(3.7)(A262T/R324L/S409I/E480K/E543D/M694I/E1219V) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized xCas9(3.7) with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   xCas9(3.7)=A262T/R324L/S409I/E480K/E543D/M694I/E1…

   Promoter

   CMV and T7

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3-myc-his-BACH1 P47A

   Plasmid

   #17644

   Depositor

   Ronny Drapkin

   Article

   Cantor et al Proc Natl Acad Sci U S A. 2004 Feb 24. 1

   Insert

   BACH1 (BRIP1 Human)
   

   Use

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   To generate the tumor-associated mutant, the prol…

   Promoter

   Available since

   April 4, 2008

   Availability

   Academic Institutions and Nonprofits only

9. pCAG-CBE4max-xCas9(3.7)-P2A-EGFP (RTW4637)

   Plasmid

   #140000

   Purpose

   CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized CBE4max xCas9(3.7) with P2A-EGFP
   

   Use

   CRISPR

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; xCas9(3.7)=A262T/R324L/S409I/E480K/…

   Promoter

   CAG

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-N2-6MYC-hMID2

    Plasmid

    #51035

    Purpose

    mammalian expression of myc-tagged human MID2

    Depositor

    Timothy Cox

    Article

    Short et al BMC Cell Biol. 2002;3:1. Epub 2002 Jan 4

    Insert

    MID2 (MID2 Human)
    

    Use

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIE-C-hα2M-H6

    Plasmid

    #128494

    Purpose

    Expresses human C-α2M (from R787 to N1473) in insect cells. With C-t H6x tag.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    A2M (R787-N1473) (A2M Human)
    

    Use

    Tags

    His tag (6x)

    Expression

    Insect

    Mutation

    contains R787-N1473

    Promoter

    IE1

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CMVenh synapsin-intron-mCherry-1x miR5 Mm ATP10B

    Plasmid

    #216391

    Purpose

    transfer plasmid for AAV vector production of mCherry and miR for Mm ATP10B

    Depositor

    Veerle Baekelandt

    Article

    Sanchiz-Calvo et al Acta Neuropathol. 2025 Jul 17;150(1):5.

    Insert

    mcherry and miR Mm ATP10B
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMVenh synapsin

    Available since

    July 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CMVenh synapsin-intron-mCherry-1x miR7 Rn ATP10B

    Plasmid

    #216392

    Purpose

    transfer plasmid for AAV vector production of mCherry and miR for rat Rn ATP10B

    Depositor

    Veerle Baekelandt

    Article

    Sanchiz-Calvo et al Acta Neuropathol. 2025 Jul 17;150(1):5.

    Insert

    mcherry and miR Rn ATP10B
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMVenh synapsin

    Available since

    July 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CMVenh synapsin-intron-mCherry-1x miR30 SCR

    Plasmid

    #216393

    Purpose

    transfer plasmid for AAV vector production of mCherry and miR scramble (no target)

    Depositor

    Veerle Baekelandt

    Article

    Sanchiz-Calvo et al Acta Neuropathol. 2025 Jul 17;150(1):5.

    Insert

    mcherry and miR control
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMVenh synapsin

    Available since

    July 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV.tetO.Nurr1

    Plasmid

    #234848

    Purpose

    3rd generation lentiviral vector, expresses Nurr1 under control of TetON promoter

    Depositor

    Malin Parmar

    Article

    Nolbrant et al Stem Cell Reports. 2020 Oct 13;15(4):869

    Insert

    Nurr1 (Nr4a2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-Syn1-DIO-Snca (human A53T)

    Plasmid

    #214201

    Purpose

    For Cre-dependent expression of alpha synuclein (human gene containing A53T mutation)

    Depositor

    Thomas Hnasko

    Article

    Steinkellner et al Brain. 2022 Apr 29;145(3):879-886. doi:

    Insert

    Snca (A53T) (SNCA Human)
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    A53T

    Promoter

    Available since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQCXIH FRB Ubiqutin-BFP

    Plasmid

    #202426

    Purpose

    TagBFP FKBP-rapamycin binding (FRB) dimerization domain fused to ubiquitin

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

    Insert

    FKBP-rapamycin binding (FRB) dimerization domain
    

    Use

    Lentiviral

    Tags

    Ubiquitin, TagBFP

    Expression

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-KrasWT/sgKras/Cre

    Plasmid

    #99848

    Purpose

    Expresses Cre-recombinase, a barcoded HDR template that serves as a control for HDR into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

    Depositor

    Monte Winslow

    Article

    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

    Insert

    Kras (Kras Mouse)
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

    Promoter

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-mEtfdh-AAA6

    Plasmid

    #232317

    Purpose

    Expression of mouse mutant Etfdh (AAA6)

    Depositor

    Ivan Topisirovic

    Article

    Papadopoli et al bioRxiv 2024.10.25.620155

    Insert

    Etfdh (Etfdh Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    PAM Sequence Mutations: (G36C; T39C, C42T; C72T, …

    Promoter

    Available since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-mEtfdh-AAG6

    Plasmid

    #232315

    Purpose

    Expression of mouse mutant Etfdh (AAG6)

    Depositor

    Ivan Topisirovic

    Article

    Papadopoli et al bioRxiv 2024.10.25.620155

    Insert

    Etfdh (Etfdh Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    PAM Sequence Mutations: (G36C, T39C, C42T, C72T, …

    Promoter

    Available since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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