We narrowed to 2,609 results for: ubiquitin

1. pOPINK-OTUD6A (full-length, aa 1-288)

   Plasmid

   #61416

   Purpose

   Expresses human OTUD6A (full-length) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUD6A (OTUD6A Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV6-AN-DDK_WT POLI

   Plasmid

   #131228

   Purpose

   Expression of WT DNA polymerase iota with an N-terminal FLAG tag in mammalian cells

   Depositor

   Roger Woodgate

   Article

   McIntyre et al Nucleic Acids Res. 2013 Feb 1;41(3):1649

   Insert

   DNA polymerase iota (POLI Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Coding sequence has been codon optimised for expr…

   Promoter

   CMV enhancer + CMV promoter

   Available since

   Dec. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-N-Myc_775-1102 USP7

   Plasmid

   #131250

   Purpose

   Mammalian expression of the USP7 UBL3-5 domains with an N-terminal Myc tag

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Encodes USP7 UBL domains 3-5 (USP7 amino acids 77…

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. PCS4 3XFLAG-PPARgamma2

   Plasmid

   #78770

   Purpose

   To overexpress PPARgamma2 in Mammalian Cells

   Depositor

   Jaewhan Song

   Article

   Kim et al Cell Death Differ. 2014 Apr;21(4):594-60

   Insert

   PPAR_2 (Ppargc1a Mouse)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available since

   June 10, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pETDUET-GST AO7REwt

   Plasmid

   #213935

   Purpose

   GST tagged AO7 encoding aa 126-258 for expression in bacteria

   Depositor

   Allan Weissman

   Article

   Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

   Insert

   AO7 aa 126-258 (RNF25 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   AO7 cloned into the first multiple cloning site o…

   Promoter

   T7 promoter-1

   Available since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p7137 pHAGE-P-CMVt-N-HA-GAW-EIF3M

   Plasmid

   #100149

   Purpose

   Expresses EIF3M

   Depositor

   Peter Howley

   Article

   Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

   Insert

   EIF3M (EIF3M Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p7129 pHAGE-P-CMVt-N-HA-GAW-HM13

   Plasmid

   #100155

   Purpose

   Expresses HM13

   Depositor

   Peter Howley

   Article

   Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

   Insert

   NM_030789.3 (HM13 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. GFP-RNF51

   Plasmid

   #126546

   Purpose

   Expresses GFP-tagged RNF51 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

   Insert

   RNF51 (BMI1 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   July 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX4T1-ISG15 C78SN13Y

   Plasmid

   #165106

   Purpose

   bacterial expressio of a GST fusion of Human ISG15 C78SN13Y

   Depositor

   Arthur Haas

   Article

   Narasimhan et al J Biol Chem. 2005 Jul 22. 280(29):27356-

   Insert

   ISG15 (ISG15 Human)
   

   Use

   Tags

   GST from plasmid.

   Expression

   Bacterial

   Mutation

   C78SN13Y; The sequence of ISG15-C78S/N13Y contai…

   Promoter

   Available since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFastbac1-GST-hUSP7

    Plasmid

    #63573

    Purpose

    Expression of synthetic human USP7 tagged with GST

    Depositor

    Titia Sixma

    Article

    Faesen et al Mol Cell. 2011 Oct 7;44(1):147-59. doi:

    Insert

    USP7 (USP7 Synthetic, Human)
    

    Use

    Tags

    GST

    Expression

    Insect

    Mutation

    Synthetic based on human protein sequence (please…

    Promoter

    Polyhedrin

    Available since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5/FRT-Flag-Cul5

    Plasmid

    #31984

    Purpose

    Mammalian expression of Flag-tagged Cul5

    Depositor

    Joan Conaway

    Article

    Mahrour et al J Biol Chem. 2008 Mar 21;283(12):8005-13

    Insert

    Cul5 (CUL5 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pCMV10-Flag-Fbxl21

    Plasmid

    #48752

    Purpose

    Expression of FLAG-tagged Fbxl21

    Depositor

    Joseph Takahashi

    Article

    Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

    Insert

    Fbxl21 (Fbxl21 Mouse)
    

    Use

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pOPINK-OTUD1 (OTU+UIM, aa 287-481)

    Plasmid

    #61406

    Purpose

    Expresses human OTUD1 (OTU+UIM domains) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD1 (OTUD1 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-286.

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5FRT/TO-UBE2QL1-CS-Strep/HA

    Plasmid

    #129513

    Purpose

    Expresses UBE2QL1 C88S (catalytically inactive mutant) with C-terminal Strep-HA tag in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

    Insert

    UBE2QL1 C88S (UBE2QL1 Human)
    

    Use

    Tags

    2xStrep/HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX-4T-1 UBXN2A

    Plasmid

    #113505

    Purpose

    Bacterial expression of GST-tagged UBXN2A

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    UBXN2A (UBXN2A Human)
    

    Use

    Tags

    GST-Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. His-SUMO- flag-hDDI2-D252N_pET11a

    Plasmid

    #169123

    Purpose

    expression of protein

    Depositor

    Barbara Dirac-Svejstrup

    Article

    Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

    Insert

    hDDI (DDI2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D252N

    Promoter

    Available since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. His-SUMO-flag-hDDI2_pET11a

    Plasmid

    #169121

    Purpose

    expression of protein

    Depositor

    Barbara Dirac-Svejstrup

    Article

    Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

    Insert

    hDDI (DDI2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p7136 pHAGE-P-CMVt-N-HA-GAW-EIF3H

    Plasmid

    #100148

    Purpose

    Expresses EIF3EH

    Depositor

    Peter Howley

    Article

    Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

    Insert

    EIF3H (EIF3H Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p3F-Strep-mMALT1

    Plasmid

    #33315

    Depositor

    Vanessa Redecke

    Article

    Stempin et al J Biol Chem. 2011 Oct 28;286(43):37147-5

    Insert

    Malt1 (Malt1 Mouse)
    

    Use

    Tags

    3xFLAG and StrepOne

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA5/FRT-Flag-NRBP1

    Plasmid

    #48197

    Purpose

    Expressed flag-tagged NRBP1 (nuclear receptor binding protein 1)

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Mahrour et al J Biol Chem. 2008 Mar 21;283(12):8005-13

    Insert

    NRBP1 (NRBP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only