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Showing: 241 - 280 of 672 results

  1. pPMVAd3

    Plasmid
    #98298
    Purpose
    Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.
    Depositor
    Claudia Vickers
    Article
    Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.
    Insert
    PRPL8B-ERG12-TNAT5-PSSB1-ERG8-TIDP1-PRPL3-MVD1-TIDP1-PYEF3-IDI1-TRPL15A
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    98298-plasmid-map-sequence-id-190181

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  2. pXWJ109As(RS-te-AAV)

    Plasmid
    #123156
    Purpose
    For TEV-based leakiness regulation. pSB3K3 carrying J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-te-AAV-t
    Depositor
    Baojun Wang
    Article
    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d
    Insert
    J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-te-AAV-t
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J109, ParsR, PhrpLE
    Available since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    123156-plasmid-map-sequence-id-240600

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  3. pXWJ109AsAmp30E

    Plasmid
    #123144
    Purpose
    Arsenic sensor with an optimized HrpRS-based amplifier. pSB3K3 carrying J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    Depositor
    Baojun Wang
    Article
    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d
    Insert
    J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J109, ParsR, PhrpLE
    Available since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    123144-plasmid-map-sequence-id-240606

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  4. As2

    Plasmid
    #123160
    Purpose
    Arsenic sensor used in the sensor cell array. pSB4A3 carrying J109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    Depositor
    Baojun Wang
    Article
    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d
    Insert
    J109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J109, ParsR-ABS84, PhrpLE
    Available since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    123160-plasmid-map-sequence-id-243993

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  5. pXWJ109HgAmp30E

    Plasmid
    #123149
    Purpose
    Mercury sensor with an optimized HrpRS-based amplifier. pSB3K3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    Depositor
    Baojun Wang
    Article
    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d
    Insert
    J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J109, PmerT, PhrpLE
    Available since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    123149-plasmid-map-sequence-id-244017

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  6. pXW109Hg(RS-RinA)

    Plasmid
    #123150
    Purpose
    Mercury sensor with two-layered amplifier cascade. pSB4A3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-t
    Depositor
    Baojun Wang
    Article
    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d
    Insert
    J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-t
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J109, PmerT, PhrpLE, PrinA
    Available since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    123150-plasmid-map-sequence-id-243991

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  8. pXW109ABS84(RS)

    Plasmid
    #123155
    Purpose
    Amplified arsenic sensor with double ArsR binding sites. pSB3K3 carrying J109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    Depositor
    Baojun Wang
    Article
    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d
    Insert
    J109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J109, ParsR-ABS84, PhrpLE
    Available since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    123155-plasmid-map-sequence-id-240627

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  9. As1

    Plasmid
    #123159
    Purpose
    Arsenic sensor used in the sensor cell array. pSB4A3 carrying J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    Depositor
    Baojun Wang
    Article
    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d
    Insert
    J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J117, ParsR, PhrpLE
    Available since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    123159-plasmid-map-sequence-id-241970

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  10. pMM0654

    Plasmid
    #129015
    Purpose
    Control for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pMEDIUM-mRUBY2 reporter
    Depositor
    Megan McClean
    Article
    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1
    Insert
    5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pRPL18B-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    129015-plasmid-map-sequence-id-259463

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  11. pXW109ABS62(RS)

    Plasmid
    #123153
    Purpose
    Amplified arsenic sensor with double ArsR binding sites. pSB3K3 carrying J109-30arsR-t-ParsR-ABS62-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    Depositor
    Baojun Wang
    Article
    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d
    Insert
    J109-30arsR-t-ParsR-ABS62-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J109, ParsR-ABS62, PhrpLE
    Available since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    123153-plasmid-map-sequence-id-241974

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  12. pXW109ABS67(RS)

    Plasmid
    #123154
    Purpose
    Amplified arsenic sensor with double ArsR binding sites. pSB3K3 carrying J109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    Depositor
    Baojun Wang
    Article
    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d
    Insert
    J109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J109. ParsR-ABS67, PhrpLE
    Available since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    123154-plasmid-map-sequence-id-240629

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  16. As0

    Plasmid
    #123158
    Purpose
    Arsenic sensor used in the sensor cell array. pSB4A3 carrying J109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-t
    Depositor
    Baojun Wang
    Article
    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d
    Insert
    J109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-t
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J109, ParsR-ABS67, PhrpLE, PrinA
    Available since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    123158-plasmid-map-sequence-id-243982

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  17. pXW117As(RS-E11)

    Plasmid
    #123145
    Purpose
    Arsenic sensor with two-layered amplifier cascade. pSB4A3 carrying J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-30gfp-t
    Depositor
    Baojun Wang
    Article
    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d
    Insert
    J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-30gfp-t
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J117, ParsR, PhrpLE, Pecf11
    Available since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    123145-plasmid-map-sequence-id-243995

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  18. pXW117As(RS-E11-RinA)2

    Plasmid
    #123146
    Purpose
    Arsenic sensor with three-layered amplifier cascade. Need to work with plasmid pXWPrinA-gfp2. pSB4A3 carrying J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-33rinA-t
    Depositor
    Baojun Wang
    Article
    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d
    Insert
    J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-33rinA-t
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J117, ParsR, PhrpLE, Pecf11
    Available since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    123146-plasmid-map-sequence-id-243978

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  19. pXW109Hg(RS-RinA-E11)2

    Plasmid
    #123151
    Purpose
    Mercury sensor with three-layered amplifier cascade. Need to work with plasmid pXWP11-gfp2. pSB4A3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-t
    Depositor
    Baojun Wang
    Article
    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d
    Insert
    J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-t
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J109, PmerT, PhrpLE, PrinA
    Available since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    123151-plasmid-map-sequence-id-244015

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  20. pStABK1

    Plasmid
    #193729
    Purpose
    Start-Stop assembly level 1 vector, BBR1 origin, kanR
    Depositor
    Martin Buck
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    193729-plasmid-map-sequence-id-381921

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  21. pPAeBlueHPV16LR

    Plasmid
    #185893
    Purpose
    2-micron plasmid of AeBlue + HPV16 L1 expression module at RPL25 locus
    Depositor
    Claudia Vickers
    Insert
    PALD6>AeBlue>TPGK1- PSe.GAL2> HPV16-L1_C-6*H > TRPL41B
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Sept. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    185893-plasmid-map-sequence-id-370866

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  22. pRPCN

    Plasmid
    #193726
    Purpose
    Expresses Cas9 from RK2 origin with RFP-marked sgRNA insertion site and kanR/sacB selection/counterselection
    Depositor
    Martin Buck
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    193726-plasmid-map-sequence-id-381907

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  23. pRPCE

    Plasmid
    #193712
    Purpose
    Expresses cytosine deaminase linked deactivated-Cas9 from RK2 origin with RFP-marked sgRNA insertion site and kanR/sacB selection/counterselection
    Depositor
    Martin Buck
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    193712-plasmid-map-sequence-id-381728

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  24. L4455

    Plasmid
    #1656
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    rpl28-14k, rpl285', SV40NLS, SV40NLS, SV40NL…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1656-plasmid-map-sequence-id-266

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  25. pRSFDuet-galK

    Plasmid
    #193727
    Purpose
    Contains expression cassette for E.coli galK for use as a counterselection marker in strains lacking galK
    Depositor
    Martin Buck
    Insert
    galK (galK E. coli)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23113
    Available since
    Feb. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    193727-plasmid-map-sequence-id-381922

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  34. StarPort01

    Plasmid
    #164503
    Purpose
    To generate a stable acceptor cell line with a BxbI attP site at the AASV1 locus, between an exogenous human pRPL30 promoter and a tripartite V5/SNAPtag/Blast reporter.
    Depositor
    Bo Huang
    Insert
    V5-SNAPtag-PDGFR_TM-T2A-Blast
    Use
    To generate attp acceptor cell lines for bxbi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human RPL30
    Available since
    Availability
    Academic Institutions and Nonprofits only
    164503-plasmid-map-sequence-id-324720

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  36. pYTK017

    Plasmid
    #65124
    Purpose
    Encodes pRPL18B as a Type 2 part to be used in the Dueber YTK system
    Depositor
    John Dueber
    Article
    Lee et al ACS Synth Biol. 2015 Apr 14.
    Insert
    pRPL18B
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    65124-plasmid-map-sequence-id-110177

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  40. StarPort02

    Plasmid
    #164504
    Purpose
    To generate a stable acceptor cell line with an improved BxbI attP-GA site at the AASV1 locus, between an exogenous human pRPL30 promoter and a tripartite V5/SNAPtag/Blast reporter.
    Depositor
    Bo Huang
    Insert
    V5-SNAPtag-PDGFR_TM-T2A-Blast
    Use
    To generate attp-ga acceptor cell lines for bxbi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human RPL30
    Available since
    Availability
    Academic Institutions and Nonprofits only
    164504-plasmid-map-sequence-id-324721

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Showing: 241 - 280 of 672 results