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Showing: 301 - 350 of 2918 results

  4. pGEM-floxedEGFP-TRI2A

    Plasmid
    #74669
    Purpose
    floxedEGFP-TRI2A in between the homology regions left (HL) and right (HR) to Rosa26 murine locus
    Depositor
    Roberto Giovannoni
    Insert
    floxedEGFP-TRI2A in between the homology regions left (HL) and right (HR) to Rosa26 murine locus
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    pCAGGS
    Available since
    Feb. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    74669-plasmid-map-sequence-id-147558

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  5. pMOTag4HM-crelox-HYG

    Plasmid
    #24029
    Depositor
    George Cross
    Type
    Empty backbone
    Use
    Cre/Lox
    Tags
    3XHA and 3XMyc
    Expression
    Mutation
    Promoter
    Available since
    Feb. 26, 2010
    Availability
    Academic Institutions and Nonprofits only
    24029-plasmid-map-sequence-id-11300

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  6. pAV10.HO3.loxP

    Plasmid
    #63205
    Purpose
    pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    63205-plasmid-map-sequence-id-104905

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  7. pAV10.HO5.loxP

    Plasmid
    #63206
    Purpose
    pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    63206-plasmid-map-sequence-id-104906

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  9. LIR-wtloxP-TRE-CAG-Frt-red-puro-3xPA-Frt-GFP(mVenus)-KrasG12D-cMyc-SV40LT-IRES-KRABoff-PA-TRE-loxP257-RIR

    Plasmid
    #67277
    Purpose
    inducbile color change and cancer regulation: FlpO recombinase dependent expression of KrasG12D cMyc and SV40 large T antigen with doxycycline inducible downregulation through tetKRAB system.
    Depositor
    Jannik Elverløv-Jakobsen
    Article
    Callesen et al Mol Oncol. 2017 Nov;11(11):1616-1629. do
    Inserts
    first casette contain a red fluorescent protein (katushka) linked to puromycin resitance gene through an E2A site
    second cassette contains mVENUS-kras-cmyc-SV40LT-KRABoff
    Use
    Tags
    mTQ2 blue fluorescent and red flourescent gene ka…
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    67277-plasmid-map-sequence-id-121189

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  10. 3` CC: Hygro – GFP-N – loxP – mScarlet

    Plasmid
    #219564
    Purpose
    3` circularization cassette.To induce Cre-mediated circularization or inversion of a genomic region with concomitant GFP reconstitution and mScarlet expression from the chromosome
    Depositor
    Andrea Ventura
    Article
    Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586
    Insert
    3` CC: Hygro – GFP-N – loxP – mScarlet
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    EF1-a
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    219564-plasmid-map-sequence-id-438335

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  17. pLinker-2xTy-HXGPRT-LoxP

    Plasmid
    #86664
    Purpose
    CRISPR tagging with 2xTy in Toxoplamsa gondii
    Depositor
    David Sibley
    Insert
    Linker-2xTy-HXGPRT 3'UTR
    Use
    Expression in toxoplasma gondii
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    86664-plasmid-map-sequence-id-193761

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  18. pET30a-His6-ABP-LOXCAT

    Plasmid
    #139971
    Purpose
    Fusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2. Contains a albumin binding peptide (ABP).
    Depositor
    Vamsi Mootha
    Article
    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103
    Insert
    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    139971-plasmid-map-sequence-id-311161

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  29. pLenti-ePEG-LINE1-loxPsym

    Plasmid
    #216866
    Purpose
    Lentiviral guide RNA vector to insert the loxPsym sequence into LINE1 elements
    Depositor
    Leopold Parts
    Insert
    spacerLINE1_cr772scaffold_PBS-loxPsm-HA-tevopreQ
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    U6
    Available since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216866-plasmid-map-sequence-id-441211

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  32. pColdII-SIRPT1-Loxodonta Africana

    Plasmid
    #75351
    Purpose
    Nt His-tag Loxodonta Africana 89-1368
    Depositor
    Kalle Gehring
    Insert
    Sacsin Loxodonta Africana 89-1368
    Use
    Tags
    His-Tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    75351-plasmid-map-sequence-id-142900

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  33. YW249_Pzipt17-loxP-H2BGFP-miniMOS

    Plasmid
    #196063
    Purpose
    a plasmid modified from miniMOS vector pCFJ910. the modification including insertions of SL2::H2B::GFP downstream to NeoR CDS and 2 loxP sites flanking the NeoR and GFP cassettes.
    Depositor
    Wenxing Yang
    Article
    Li et al PeerJ. 2023 May 17;11:e15433. doi: 10.77
    Inserts
    H2B::GFP
    NeoR
    Minimal Mos1
    zipt17p::zipt17::zipt17 3UTR
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Jan. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    196063-plasmid-map-sequence-id-383077

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  35. pET30a-His6-ABP-LOXCATmut

    Plasmid
    #139972
    Purpose
    A control for His6-ABP-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)
    Depositor
    Vamsi Mootha
    Article
    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103
    Insert
    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Mutation in H265A and R268A in LOX (used original…
    Promoter
    T7
    Available since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    139972-plasmid-map-sequence-id-311162

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  37. pLinker-6xHA-HXGPRT-LoxP

    Plasmid
    #86552
    Purpose
    The plasmid is used for endogenous tagging using CRISPR technology in Toxoplasma
    Depositor
    David Sibley
    Insert
    Linker-6xHA-HGPRT-LoxP
    Use
    Expression in toxoplasma gondii
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    86552-plasmid-map-sequence-id-190138

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  44. pMuLE ENTR CMV loxP L5-L2

    Plasmid
    #62094
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and multiple cloning site flanked by loxP sites. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Article
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Type
    Empty backbone
    Use
    Cre/Lox; Mule gateway entry vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62094-plasmid-map-sequence-id-101383

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Showing: 301 - 350 of 2918 results