Skip to main content
Addgene
Showing: 301 - 350 of 57713 results

  1. pDD346

    Plasmid
    #133311
    Purpose
    SapTrap mNG-C1 donor
    Depositor
    Daniel Dickinson
    Insert
    C. elegans codon-optimized mNeonGreen
    Use
    CRISPR; Saptrap donor for cloning repair templat…
    Tags
    C. elegans codon-optimized mNeonGreen
    Expression
    Worm
    Mutation
    Promoter
    None
    Available since
    Nov. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    133311-plasmid-map-sequence-id-263341

    Enlarge

  2. pDD373

    Plasmid
    #137021
    Purpose
    SapTrap GFP-GLO donor
    Depositor
    Daniel Dickinson, Bob Goldstein
    Insert
    C. elegans GLO-optimized GFP
    Use
    CRISPR; Saptrap donor for cloning repair templat…
    Tags
    C. elegans GLO-optimized GFP
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    137021-plasmid-map-sequence-id-274046

    Enlarge

  3. pDD376

    Plasmid
    #137022
    Purpose
    SapTrap mKate2-GLO donor
    Depositor
    Daniel Dickinson, Bob Goldstein
    Insert
    C. elegans GLO-optimized mKate2
    Use
    CRISPR; Saptrap donor for cloning repair templat…
    Tags
    C. elegans GLO-optimized mKate2
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    137022-plasmid-map-sequence-id-274081

    Enlarge

  4. pDD362

    Plasmid
    #137023
    Purpose
    SapTrap HaloTag-GLO donor
    Depositor
    Daniel Dickinson, Bob Goldstein
    Insert
    C. elegans GLO-optimized Halo Tag
    Use
    CRISPR; Saptrap donor for cloning repair templat…
    Tags
    C. elegans GLO-optimized Halo Tag
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    137023-plasmid-map-sequence-id-274041

    Enlarge

  5. PAP39A

    Plasmid
    #42342
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PAP39A (PRPSAP1 Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  6. pLIB_BSSHI_6xHisTEV-WIPI2d

    Plasmid
    #190863
    Purpose
    Plasmid for the expression of purification of WIPI2d: SMC Internal reference: SMC1216
    Depositor
    Sascha Martens
    Insert
    WIPI2 (WIPI2 Human)
    Use
    Tags
    Expression
    Bacterial and Insect
    Mutation
    SNP C to G at position 4562 (Val)
    Promoter
    Available since
    Sept. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    190863-plasmid-map-sequence-id-373591

    Enlarge

  7. His 3C MKK3 1-347

    Plasmid
    #87878
    Purpose
    E. coli expression of MKK3 aa 1-347
    Depositor
    Peter Mace
    Insert
    MKK3 (MAP2K3 Human)
    Use
    Tags
    3C protease site and 6xHis
    Expression
    Bacterial
    Mutation
    aa 1-347
    Promoter
    T7
    Available since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87878-plasmid-map-sequence-id-174923

    Enlarge

  8. SF3A1

    Plasmid
    #25608
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SF3A1 (SF3A1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Ubiquitin-like domain
    Promoter
    Available since
    Aug. 30, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  9. MAPK8_BirA_Cterm

    Plasmid
    #221519
    Purpose
    Flag-tagged biotinylation enzyme for co-expression of Mapk8
    Depositor
    Alessandro Ori
    Insert
    MAPK8 (MAPK8 Human)
    Use
    Tags
    BirA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    221519-plasmid-map-sequence-id-439379

    Enlarge

  10. His 3C MKK3 42-347

    Plasmid
    #87879
    Purpose
    E. coli expression of MKK3 aa 42-347
    Depositor
    Peter Mace
    Insert
    MKK3 (MAP2K3 Human)
    Use
    Tags
    3C protease site and 6xHis
    Expression
    Bacterial
    Mutation
    aa 42-347
    Promoter
    T7
    Available since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87879-plasmid-map-sequence-id-174922

    Enlarge

  11. His 3C MKK6 1-334

    Plasmid
    #87881
    Purpose
    E. coli expression of MKK6 aa 1-334
    Depositor
    Peter Mace
    Insert
    MKK6 (MAP2K6 Human)
    Use
    Tags
    3C protease site and 6xHis
    Expression
    Bacterial
    Mutation
    aa 1-334
    Promoter
    T7
    Available since
    March 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    87881-plasmid-map-sequence-id-174845

    Enlarge

  13. pBABE 3xHA-MKK6-EE puro

    Plasmid
    #216791
    Purpose
    Mammalian expression of constitutively active human MKK6
    Depositor
    Kevin Janes
    Insert
    MKK6 (MAP2K6 Human)
    Use
    Retroviral
    Tags
    3x-HA
    Expression
    Mammalian
    Mutation
    S207E and T211E, constitutively active
    Promoter
    LTR
    Available since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216791-plasmid-map-sequence-id-434910

    Enlarge

  14. pGB-06-07-Vps34

    Plasmid
    #187989
    Purpose
    Plasmid for the protein expression of Vps34 in a bacterial and insect expression system. Internal_Reference: SMC1323
    Depositor
    Sascha Martens
    Insert
    Vps34 (PIK3C3 Human)
    Use
    Tags
    Expression
    Bacterial and Insect
    Mutation
    Promoter
    Available since
    Feb. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    187989-plasmid-map-sequence-id-374438

    Enlarge

  15. pCSmp38ERT(#143)

    Plasmid
    #184077
    Purpose
    cyclofen-inducible P38 activation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Insert
    5myc-ZfP38a-ERT2
    Use
    Synthetic Biology
    Tags
    5xMyc
    Expression
    Mammalian
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    sCMV+SP6
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184077-plasmid-map-sequence-id-365706

    Enlarge

  18. His 3C MKK4 20-399

    Plasmid
    #87880
    Purpose
    E. coli expression of MKK4 aa 20-399
    Depositor
    Peter Mace
    Insert
    MKK4 (MAP2K4 Human)
    Use
    Tags
    3C protease site and 6xHis
    Expression
    Bacterial
    Mutation
    aa 20-399
    Promoter
    T7
    Available since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87880-plasmid-map-sequence-id-174924

    Enlarge

  19. pGB-05-08-Vps15

    Plasmid
    #187990
    Purpose
    Plasmid for the protein expression of Vps15 in a bacterial and insect expression system. Internal_Reference: SMC1324
    Depositor
    Sascha Martens
    Insert
    Vps15 (PIK3R4 Human)
    Use
    Tags
    Expression
    Bacterial and Insect
    Mutation
    Promoter
    Available since
    Feb. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    187990-plasmid-map-sequence-id-374169

    Enlarge

  20. pCSm5En2DER4(#1062)

    Plasmid
    #184093
    Purpose
    cyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Insert
    5myc-chkEn2(C>D)-ERT2
    Use
    Synthetic Biology
    Tags
    5xMyc
    Expression
    Mammalian
    Mutation
    chkEn2: C175D; ERT2: G400V, M543A, L544A, deleted…
    Promoter
    sCMV+SP6
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184093-plasmid-map-sequence-id-365715

    Enlarge

  25. pGB-08-09-BECN1

    Plasmid
    #187991
    Purpose
    Plasmid for the protein expression of BECN1/Beclin1 in a bacterial and insect expression system. Internal_Reference: SMC1325
    Depositor
    Sascha Martens
    Insert
    BECN1 (BECN1 Human)
    Use
    Tags
    Expression
    Bacterial and Insect
    Mutation
    Promoter
    Available since
    Feb. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    187991-plasmid-map-sequence-id-374168

    Enlarge

  26. pGB-05-06-GST-ATG14

    Plasmid
    #187987
    Purpose
    Plasmid for the protein expression of GST-ATG14 in a bacterial and insect expression system. Internal_Reference: SMC1321
    Depositor
    Sascha Martens
    Insert
    ATG14 (ATG14 Human)
    Use
    Tags
    GST
    Expression
    Bacterial and Insect
    Mutation
    Promoter
    Available since
    Feb. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    187987-plasmid-map-sequence-id-374440

    Enlarge

  29. pMLS256

    Plasmid
    #73715
    Purpose
    SapTrap destination vector for building combined sgRNA expression + repair template vectors
    Depositor
    Erik Jorgensen
    Article
    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.
    Insert
    pU6::sgRNA
    Use
    CRISPR
    Tags
    Expression
    Worm
    Mutation
    SapI insertion site
    Promoter
    C. elegans U6 snRNA pol III promoter
    Available since
    March 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    73715-plasmid-map-sequence-id-200872

    Enlarge

  31. MAPK11

    Plasmid
    #39005
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MAPK11 (MAPK11 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  32. UPLC1

    Plasmid
    #25550
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    UPLC1 (ASAP3 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 25, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  33. pGB-05-06-GST-mCherry-ATG14

    Plasmid
    #187988
    Purpose
    Plasmid for the protein expression of GST-mCherry-ATG14 in a bacterial and insect expression system. Internal_Reference: SMC1322
    Depositor
    Sascha Martens
    Insert
    ATG14 (ATG14 Human)
    Use
    Tags
    GST-mCherry
    Expression
    Insect and Mammalian
    Mutation
    Promoter
    Available since
    Feb. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    187988-plasmid-map-sequence-id-374439

    Enlarge

  38. MAPK8

    Plasmid
    #73236
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MAPK8 (MAPK8 Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    pfam00069:Pkinase 26-321
    Promoter
    T7
    Available since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73236-plasmid-map-sequence-id-137188

    Enlarge

  42. pT2iU6m5Hoxa13bE4PCh(#765)

    Plasmid
    #184102
    Purpose
    cyclofen-inducible fish Hoxa13b activation via zebrafish transient transgenesis or mRNA synthesis
    Depositor
    Sophie Vriz
    Insert
    5myc-ZfHoxa13b-ERT2-P2A-mCherry
    Use
    Zebrafish transgenesis (blue eyes) + in vitro tra…
    Tags
    5xMyc
    Expression
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    Zf-Ubi+SP6
    Available since
    July 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    184102-plasmid-map-sequence-id-365950

    Enlarge

  44. pT22i5uasm5En25EE4P3Che(#1213)

    Plasmid
    #184099
    Purpose
    cyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenic
    Depositor
    Sophie Vriz
    Insert
    5myc-chkEn2(5E)-ERT2-5'fP2A-mCherry
    Use
    Zebrafish transgenesis (blue eyes)
    Tags
    5xMyc
    Expression
    Mutation
    chkEn2: S150E, S151E, S153E, S155E, S156E; ERT2: …
    Promoter
    5xUAS
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184099-plasmid-map-sequence-id-364013

    Enlarge

  45. pT22i5uasm5En2SE4P3Che(#1209)

    Plasmid
    #184097
    Purpose
    cyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenic
    Depositor
    Sophie Vriz
    Insert
    5myc-chkEn2(C>S)-ERT2-5'fP2A-mCherry
    Use
    Zebrafish transgenesis (blue eyes)
    Tags
    5xMyc
    Expression
    Mutation
    chkEn2: C175S; 5'fP2A: GSG-P2A; ERT2: G400V,…
    Promoter
    5xUAS
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184097-plasmid-map-sequence-id-364012

    Enlarge

  46. pT22cUm5En2SE4P3Che(#1189)

    Plasmid
    #184095
    Purpose
    cyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenic
    Depositor
    Sophie Vriz
    Insert
    5myc-chkEn2(C>S)-ERT2-5'fP2A-mCherry
    Use
    Zebrafish transgenesis (blue eyes)
    Tags
    5xMyc
    Expression
    Mutation
    chkEn2: C175S; 5'fP2A: GSG-P2A; ERT2: G400V,…
    Promoter
    Zf-Ubi
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184095-plasmid-map-sequence-id-363968

    Enlarge

  47. pT22cUm5En2E4P3Che(#1188)

    Plasmid
    #184094
    Purpose
    cyclofen-inducible chicken EN2 (wt) activation in zebrafish permanent transgenic
    Depositor
    Sophie Vriz
    Insert
    5myc-chkEn2-ERT2-5'fP2A-mCherry
    Use
    Zebrafish transgenesis (blue eyes)
    Tags
    5xMyc
    Expression
    Mutation
    5'fP2A: GSG-P2A; ERT2: G400V, M543A, L544A, …
    Promoter
    Zf-Ubi
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184094-plasmid-map-sequence-id-363967

    Enlarge

  48. pT22i5uasm5En2E4P3Che(#1208)

    Plasmid
    #184096
    Purpose
    cyclofen-inducible chicken EN2 (wt) activation in zebrafish permanent transgenic
    Depositor
    Sophie Vriz
    Insert
    5myc-chkEn2-ERT2-5'fP2A-mCherry
    Use
    Zebrafish transgenesis (blue eyes)
    Tags
    5xMyc
    Expression
    Mutation
    5'fP2A: GSG-P2A; ERT2: G400V, M543A, L544A, …
    Promoter
    5xUAS
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184096-plasmid-map-sequence-id-363966

    Enlarge

  50. MAP2K6

    Plasmid
    #38997
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MAP2K6 (MAP2K6 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Activation loop mutant S207D/T211D
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 301 - 350 of 57713 results