Addgene

We narrowed to 2,521 results for: YAO

Showing: 301 - 350 of 2521 results

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pSF11-wmiRFP680-T2A-HO1

Plasmid

#197244

Purpose

Expresses the protein of wmiRFP680-T2A-HO1 in neurons of C elegan

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

wmiRFP680-T2A-HO1

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
p23-DDX21 DEV

Plasmid

#128805

Purpose

overexpression of DDX21 DEV mutation in human cells

Depositor

Ling-Ling Chen

Article

Xing et al Cell. 2017 May 4;169(4):664-678.e16. doi

Insert

DDX21 DEV mutation

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CAG-miRFP713

Plasmid

#197208

Purpose

Expresses the protein of miRFP713 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP713

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(cyto).cpSFGFP.HaloTag

Plasmid

#209662

Purpose

Non-responsive control

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

cpSFGFP.HaloTag

Use

AAV

Tags

Expression

Mutation

Promoter

GFAP

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-emiRFP703

Plasmid

#197205

Purpose

Expresses the protein of emiRFP703 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

emiRFP703

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-Tight2-AkaLuc

Plasmid

#186186

Purpose

AAV vector packaging AkaLuciferase under the TRE promoter

Depositor

Vladislav Verkhusha

Article

Kasatkina et al Nat Commun. 2022 May 19;13(1):2813. doi:

Insert

AkaLuc luciferase

Use

AAV

Tags

Expression

Mutation

Promoter

TRE-Tight2

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pH2B-miRFP670-2-N1

Plasmid

#197228

Purpose

Expresses the fusion protein of H2B-miRFP670-2 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

H2B-miRFP670-2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pmiRFP680-Actin-C-18

Plasmid

#197219

Purpose

Expresses the fusion protein of miRFP680-Actin-C-18 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP680-Actin-C-18

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET151-mUCH37 (ISF2)

Plasmid

#61928

Purpose

Expression of mUCH37 (ISF2) in E.coli

Depositor

Chris Hill

Article

VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

Insert

UCH37 (M.musculus) (Uchl5 Mouse)

Use

Tags

6x HIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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LovTAP N538E

Plasmid

#61431

Purpose

Expresses calmodulin binding protein-LovTAP N538E fusion protein in E. coli cells. LovTAP N358E is a optogenetic protein that can repress the Trp promoter in the presence of blue light.

Depositor

Tobin Sosnick

Article

Strickland et al Nat Methods. 2010 Aug;7(8):623-6. doi: 1

Insert

LovTAP N538E

Use

Tags

CBP

Expression

Bacterial

Mutation

Promoter

T7 lac operator

Available since

June 9, 2016

Availability

Academic Institutions and Nonprofits only
pk335-BFP

Plasmid

#227505

Purpose

Backbone for CARGO cloning, with U6 promoter and gRNA scaffold, TagBFP, and Kanamycin resistence

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-SNIFP

Plasmid

#197210

Purpose

Expresses the protein of SNIFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

SNIFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pSF11-wmCardinal-T2A-HO1

Plasmid

#197249

Purpose

Expresses the protein of wmCardinal-T2A-HO1 in neurons of C. elegans

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

wmCardinal-T2A-HO1

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSF11-wmiRFP720-T2A-HO1

Plasmid

#197248

Purpose

Expresses the protein of wmiRFP720-T2A-HO1 in neurons of C. elegans

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

wmiRFP720-T2A-HO1

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CAG-mIFP

Plasmid

#197214

Purpose

Expresses the protein of mIFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

mIFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

April 4, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pKeratin-miRFP670-2-N1

Plasmid

#197233

Purpose

Expresses the fusion protein of Keratin-miRFP670-2 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

Keratin-miRFP670-2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCx43-7-miRFP670-2-N1

Plasmid

#197223

Purpose

Expresses the fusion protein of Cx43-7-miRFP670-2 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

Cx43-7-miRFP670-2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pmiRFP720-Actin-C-18

Plasmid

#197221

Purpose

Expresses the fusion protein of miRFP720-Actin-C-18 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP720-Actin-C-18

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCx43-7-miRFP680-N1

Plasmid

#197224

Purpose

Expresses the fusion protein of Cx43-7-miRFP680 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

Cx43-7-miRFP680

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pmiRFP713-Actin-C-18

Plasmid

#197220

Purpose

Expresses the fusion protein of miRFP713-Actin-C-18 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP713-Actin-C-18

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCx43-7-miRFP720-N1

Plasmid

#197226

Purpose

Expresses the fusion protein of Cx43-7-miRFP720 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

Cx43-7-miRFP720

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCx43-7-iRFP670-N1

Plasmid

#197222

Purpose

Expresses the fusion protein of Cx43-7-iRFP670 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

Cx43-7-iRFP670

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCx43-7-miRFP713-N1

Plasmid

#197225

Purpose

Expresses the fusion protein of Cx43-7-miRFP713 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

Cx43-7-miRFP713

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
piRFP670-Actin-C-18

Plasmid

#197217

Purpose

Expresses the fusion protein of iRFP670-Actin-C-18 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

iRFP670-Actin-C-18

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pmiRFP670-2-Actin-C-18

Plasmid

#197218

Purpose

Expresses the fusion protein of miRFP670-2-Actin-C-18 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP670-2-Actin-C-18

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSF11-wiRFP713-T2A-HO1

Plasmid

#197247

Purpose

Expresses the protein of wiRFP713-T2A-HO1 in neurons of C. elegans

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

wiRFP713-T2A-HO1

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CAG-iRFP713

Plasmid

#197209

Purpose

Expresses the protein of iRFP713 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

iRFP713

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CAG-BDFP1.8

Plasmid

#197206

Purpose

Expresses the protein of BDFP1.8 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

BDFP1.8

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CAG-iRFP682

Plasmid

#197204

Purpose

Expresses the protein of iRFP682 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

iRFP682

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CAG-mRhubarb719

Plasmid

#197211

Purpose

Expresses the protein of mRhubarb719 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

mRhubarb719

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CAG-miRFP2

Plasmid

#197216

Purpose

Expresses the protein of miRFP2 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP2

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CAG-IFP2

Plasmid

#197215

Purpose

Expresses the protein of IFP2 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

IFP2

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSF11-wmRhubarb713-T2A-HO1

Plasmid

#197245

Purpose

Expresses the protein of wmRhubarb713-T2A-HO1 in neurons of C elegan

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

wmRhubarb713-T2A-HO1

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCytERM-miRFP670-2-N1

Plasmid

#197181

Purpose

Expresses the fusion protein of CytERM-miRFP670-2 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

CytERM-miRFP670-2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCytERM-E2-Crimson-N1

Plasmid

#197177

Purpose

Expresses the fusion protein of CytERM-E2-Crimson in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

CytERM-E2-Crimson

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pB2AR-miRFP718nano

Plasmid

#195751

Purpose

Beta-2-adrenergic receptor internally tagged with miRFP718nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

Insert

B2AR-miRFP718nano

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD/His-miRFP718nano

Plasmid

#195743

Purpose

Monomeric near-infrared fluorescent protein miRFP718nano in bacterial plasmid

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

Insert

miRFP718nano

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

araC

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGas-miRFP718nano

Plasmid

#195752

Purpose

G protein alpha-subunit internally tagged with miRFP718nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

Insert

G protein alpha-s-miRFP718nano

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS-ATG1(mut)(406)

Plasmid

#122069

Purpose

Expressing a mutant ATG1

Depositor

Daniel Klionsky

Article

Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

Insert

ATG1 (ATG1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Codon changes without changing the amino acid seq…

Promoter

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS-ATG7p-ATG1(406)

Plasmid

#122071

Purpose

Expressing ATG1

Depositor

Daniel Klionsky

Article

Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

Insert

ATG1 (ATG1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

The native promoter was replaced by ATG7 promoter…

Promoter

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS-Dhh1-PA(D195A,E196A)(405)

Plasmid

#122065

Purpose

Expressing Dhh1-PA

Depositor

Daniel Klionsky

Article

Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

Insert

Dhh1-PA (DHH1 Budding Yeast)

Use

Tags

PA

Expression

Yeast

Mutation

Aspartic acid 195 to alanine; Glutamic acid 196 t…

Promoter

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS-Dhh1-PA(S226A,T228A)(405)

Plasmid

#122066

Purpose

Expressing Dhh1-PA

Depositor

Daniel Klionsky

Article

Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

Insert

Dhh1-PA (DHH1 Budding Yeast)

Use

Tags

PA

Expression

Yeast

Mutation

Serine 226 to alanine; Threonine 228 to alanine

Promoter

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET151-hUCH37 (ISF2)

Plasmid

#61930

Purpose

Expression of hUCH37 (ISF2) in E.coli

Depositor

Chris Hill

Article

VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

Insert

hUCH37 (ISF1) (UCHL5 Human)

Use

Tags

6x HIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
pLVX-TetOne-Puro-mitoLbNOX-FLAG

Plasmid

#218667

Purpose

pLVX-TetOne-Puro-mitoLbNOX-FLAG

Depositor

Valentin Cracan

Article

Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

Insert

mitoLbNOX

Use

Lentiviral and Synthetic Biology

Tags

FLAG and MTS

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-TetOne-Puro-LbNOX-FLAG

Plasmid

#218631

Purpose

This plasmid contains LbNOX under doxycycline inducible control.

Depositor

Valentin Cracan

Article

Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

Insert

LbNOX

Use

Lentiviral and Synthetic Biology

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.CAG.(cyto).iATPSnFR2.A95K.HaloTag

Plasmid

#209652

Purpose

Expression of iATPSnFR2, medium affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95K.HaloTag

Use

AAV

Tags

Expression

Mutation

A95K

Promoter

CAG

Available since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-NLS-dPspCas13b-3xmRuby3-NLS

Plasmid

#132405

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

H133A; H1059A

Promoter

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.CAG.(cyto).iATPSnFR2.S29W.A95K.HaloTag

Plasmid

#209651

Purpose

Expression of iATPSnFR2, high affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.S29W.HaloTag

Use

AAV

Tags

Expression

Mutation

S29W.A95K

Promoter

CAG

Available since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pspCas9-HF-tetra-com-vector

Plasmid

#132555

Purpose

Vector plasmid expressing high fidelity spCas9 (R691A) and sgRNA scaffold with com replacing the Tetraloop.

Depositor

Baisong Lu

Article

Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

Inserts

High fidelity Cas9
sgRNA scaffold with com replacing the Tetraloop

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.CAG.(cyto).iATPSnFR2.A95A.A119L.HaloTag

Plasmid

#209653

Purpose

Expression of iATPSnFR2, low affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95A.A119L.HaloTag

Use

AAV

Tags

Expression

Mutation

A95A.A119L

Promoter

CAG

Available since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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